| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,065,799 | T→G | 73.7% | F150V (TTT→GTT) | rcsC_6 → | Sensor histidine kinase RcsC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,065,799 | 0 | T | G | 73.7% | 33.1 / 10.6 | 26 | F150V (TTT→GTT) | rcsC_6 | Sensor histidine kinase RcsC |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (12/7); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAGCCG > ACB122/3065671‑3065925 | ccGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGt < 8:43572/133‑1 (MQ=255)ccGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGt > 7:43572/1‑133 (MQ=255) cTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGCCATTa > 3:77088/1‑136 (MQ=255) cGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGGGCTGATGAACCTGCTGCTCAAACCCGGCCATGACGGGCTGGTGGAGGGGGTTGTCGCCGACATTAcc < 5:6684/136‑1 (MQ=255) cGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGCCGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAAc > 3:200349/1‑136 (MQ=255) cGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAAc > 5:59407/1‑136 (MQ=255) gAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGACATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCa < 4:50832/136‑1 (MQ=255) gCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAg > 4:4771/1‑136 (MQ=255) cACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTGGCCca < 4:189804/134‑1 (MQ=255) ggCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCagcagc > 1:148537/1‑136 (MQ=255) aCGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGcc > 2:162359/1‑135 (MQ=255) aCGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGCGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCt > 5:88583/1‑136 (MQ=255) gAAACCCGTCTGCGGTGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGACCGCAAGCTGGCCCAGCAGCGCCTGc > 5:16938/1‑136 (MQ=255) gAAACCCGGCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGc < 5:162594/136‑1 (MQ=255) cgcAAGGAGGGCAGCCTTCTCTTTGTTCTGATGATCGTGCTGCTCAAACCCGGCCATGAAGGTCTGGTGGAGGGGGTTGTCGCCGTCATTACCGAAGGCAAGCTGGCCCAGCAGAGCCTGCAACAACTCAATGACg < 6:88583/136‑1 (MQ=255) cAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGaa > 3:138932/1‑136 (MQ=255) gCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACg > 7:63471/1‑136 (MQ=255) gCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACg > 7:155006/1‑136 (MQ=255) atgaACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACG‑AAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGa < 7:138724/136‑1 (MQ=255) gctCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTg > 5:93838/1‑136 (MQ=255) ctCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTg < 3:4771/135‑1 (MQ=255) ctCAAACCCGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTACAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTg < 1:162359/135‑1 (MQ=255) ctCAAACCCCGCCAGTACTGGCTGGGGGAGGGGGTTGTCGCCGACATTACCGACCGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGGACCGATGAATTGCt < 2:165399/134‑1 (MQ=255) ccGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTgcgc > 1:180559/1‑111 (MQ=255) ccGGCCATGAAGGGCTGGTGGAGGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTgcgc < 2:180559/111‑1 (MQ=255) gtggtgGGGGTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAgccg > 1:179106/1‑136 (MQ=255) | CCGCGGTGCTCGCCCGTGAGCACAGCCTGCACGGCTACGAAACCCGTCTGCGGCGCAAGGACGGCAGCCATCTCGATGTGCTGATGAACCTGCTGCTCAAACCCGGCCATGAAGGGCTGGTGGAGGGGTTTGTCGCCGACATTACCGAACGCAAGCTGGCCCAGCAGCGCCTGCAACAACTCAATGACGAACTGGAGCAACGGGTTGCTGCGCGTACCGATGAATTGCTGGAGGCGCGTGATGCCGCCGAAGCCG > ACB122/3065671‑3065925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |