| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 3,143,328 | G→C | 18.2% | A125A (GCG→GCC) | CJ019_02960 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,143,328 | 0 | G | C | 18.2% | 54.2 / 6.7 | 22 | A125A (GCG→GCC) | CJ019_02960 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (9/9); new base C (2/2); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
TGGCTGGGCGGCGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGC > ACB122/3143200‑3143458 | tGGCTGGGCGGCGTCGCGGCCCTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGGAGGTCAGGATGTGGTGGAGCGGGTGCTTGCGAGGGGtg > 2:91470/1‑135 (MQ=255)tGGCTGGGCGGCGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGt > 4:103450/1‑135 (MQ=255) cTGGGCGGCGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATg > 1:79068/1‑136 (MQ=255) gggCGGCGTCGCGGCACTGCTCGATCCGCTGATGCCCGCCGCGGAGCAACTGACGCTGTTGCGTGACCTGCAGGCCGGTTTCGTGGTTGCGGCAGGTCCGGAGGAGGGCGCGCGGGTGCTGGCCCGCGGTGATGtg > 2:93405/1‑135 (MQ=255) gggCGGCGTCGCGGCACTGCTCGATCCGCTGATGCCCGCCGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTGGTTTCCGAAGGGGAGGCGGAGGTCGCGCGGGTGCTGGCCCGCGGGCATGTa > 2:93407/1‑136 (MQ=255) ggcggcGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTAc < 8:65877/136‑1 (MQ=255) ggcggcGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTAc < 6:84583/136‑1 (MQ=255) ggcggcGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTAc < 6:83531/136‑1 (MQ=255) ggcggcGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTAc < 6:130104/136‑1 (MQ=255) ggcggcGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTAc < 1:91470/136‑1 (MQ=255) gATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCCAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGt < 1:93407/136‑1 (MQ=255) gATGCCCGACGCGGAGCAACTGACGCTGGTGCGTGAACTGCAGGCCGGTGTCGTCTTTGCCGAAGGTCAGGAGGAGGGCGAGCGGGTGCTGGCCAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGt < 1:93405/136‑1 (MQ=255) aCTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCggg < 2:79068/136‑1 (MQ=255) aCTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGc > 8:65129/1‑136 (MQ=255) cAGGCCGGTTTCGTCTTTGCCGTAGGGCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTgg < 3:29241/136‑1 (MQ=255) cGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTg > 3:80382/1‑136 (MQ=255) tCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTg > 8:139675/1‑136 (MQ=255) cAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTgg > 8:161168/1‑136 (MQ=255) aggaggTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGt < 7:65129/136‑1 (MQ=255) gAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGTTGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGgcgc > 8:57810/1‑136 (MQ=255) gCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAgagcgcg > 3:36000/1‑134 (MQ=255) cTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAgagcgagc < 6:67232/136‑1 (MQ=255) | TGGCTGGGCGGCGTCGCGGCACTGCTCGATCCGCTGATGCCCGACGCGGAGCAACTGACGCTGTTGCGTGAACTGCAGGCCGGTTTCGTCTTTGCCGAAGGTCAGGAGGAGGTCGAGCGGGTGCTGGCGAGCGGTCATGTACCGGCATTGCTGTTCTACGCCGATGGCCGTGGCCTGGCGGATTCCGGGTTGCCGAATTACGCTCGCCTGGCCGCGCAGCTGCCCGATCCTGCCTTGGCGGTGAAGGCGCAGAGCGAGC > ACB122/3143200‑3143458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |