| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,135,607 | A→C | 51.5% | Q32P (CAG→CCG) | tgnC → | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,135,607 | 0 | A | C | 51.5% | ‑2.0 / 5.5 | 17 | Q32P (CAG→CCG) | tgnC | (Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (5/3); new base C (7/2); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01 | |||||||||||
CCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAG > ACB122/4135483‑4135725 | ccTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCCGCCCGGgcgc > 7:131713/1‑136 (MQ=255) cTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGCCCTTCGACAGCCTCAACCCGGCCCCCGCCCCGGCCGGGgcgcc > 1:65178/1‑135 (MQ=255) aaTCCGCCAAGGGGATTGTTGCATGACCCTCGTGCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGAGGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGCCCACCGCCCAGGCCTGGGCGCAACTGccc < 4:65935/136‑1 (MQ=255) aTCCGCCATGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCg < 7:46432/136‑1 (MQ=255) cAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCg > 2:167404/1‑136 (MQ=255) gACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCTGGGCGCAACAGCCCGACGCCGATGAAGCCGATGTGGAg > 8:42208/1‑136 (MQ=255) gCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGGCCAGGCCGGGGCGCACCTGCCCGACGCCGAAGACGGCGATGTGGCGCAGGACGGTCTGGCCGCCCACCGGGCCGTCGa > 7:9627/1‑136 (MQ=255) agagCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCCACGGCCCGCCGCCGAGGAAGGCGATGTGGAGCTGGCCGTTCAGGCCCCCCCGCGGGCCGTCGCCCGCAAGGCGGGgggcgg > 3:20242/1‑134 (MQ=255) gCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCACGCCGGGGCGCCCCGGCCCGCCCCCGCGGTCCCCGATGGTGGGCTGGCCGGTGAGGCCGCCCCCCCTGCCGTCGCCCGCAAGGCGGGGCgccgcag > 2:92025/1‑135 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACGGCCCCCCGCCGAGGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGACAGCAAGGCGTGGCGCAGCATCACCGc > 7:32545/1‑135 (MQ=255) gACCTTCGACAGCCTCAACCCGGCCACCACCCACGCCAAGGACCAACACCCCCACCCCGATGAAGCCCATGTGGAGCAGGCCCTTCAGGCCGCCCAGCCTGCCATCGACCCCCACGCCTTGCGCAGCATcccccc > 2:135960/1‑133 (MQ=255) cTTCGACAGCCTCAACCCGGCCCCCGCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAcc < 7:9384/136‑1 (MQ=255) cAGCCTCAACCCGGCCACCGCCCCGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTAccgcccgc > 8:69063/1‑136 (MQ=255) cAGCCTCAACCCGGCCACCGCCCCGGCCGGGGCGCCACTGCCCGCCGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCGTCGACAGCAAGGCGTGGCGCCGCATCACCGCTAcccccccc > 2:64821/1‑134 (MQ=255) cgCCACAACCCGCCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 4:115193/135‑1 (MQ=255) ccccTCACCCCGCCCCCCCCCCCGGCCGGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCcgcg < 1:64821/134‑1 (MQ=255) cAACCCGGCCACCGCCCCGGCCTGGGCGCAACAGCCCGACGCCGATGAAGCCGATGTGGCGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGCCAGCAAGGCGTGGCGCAGCATCCCCGCTACCGCCCGCGGCAAg > 2:145899/1‑136 (MQ=255) | CCTCACCGAATCCGCCAAGGGGATTGTTGCATGACCCTCGTTCGTTTCCAGATGTGCATCGACGGCCAATGGCGCGATGCCCAGAGCGGCAAGACCTTCGACAGCCTCAACCCGGCCACCGCCCAGGCCTGGGCGCAACTGCCCGACGCCGATGAAGCCGATGTGGAGCTGGCCGTTCAGGCCGCCCAGCGTGCCTTCGACAGCAAGGCGTGGCGCAGCATCACCGCTACCGCCCGCGGCAAG > ACB122/4135483‑4135725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |