Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,487,862 C→A 9.6% V186V (GTC→GTA)  CJ019_04257 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,487,8620CA 9.6% 114.2 / 3.8 42V186V (GTC→GTA) CJ019_04257hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (18/20);  new base A (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

GATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCAATCCTTATCTAAT  >  ACB122/4487727‑4487993
                                                                                                                                       |                                                                                                                                   
gaTCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTc                                                                                                                                     <  6:67839/136‑1 (MQ=255)
        aCGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAggg                                                                                                                             <  4:9165/136‑1 (MQ=255)
           ccGTTTACGATCGTGCTGCCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCaa                                                                                                                          <  1:149342/136‑1 (MQ=255)
            cGTTTACGATCGTGCTGCCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGc                                                                                                                            >  8:156608/1‑133 (MQ=255)
                           tgcCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTg                                                                                                          >  7:139852/1‑136 (MQ=255)
                           tgcCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTg                                                                                                          >  1:97321/1‑136 (MQ=255)
                           tgcCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTg                                                                                                          >  5:87738/1‑136 (MQ=255)
                           tgcCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTg                                                                                                          >  4:95259/1‑136 (MQ=255)
                                               cTATGGATTTGGCCAACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCgg                                                                                      <  1:136516/136‑1 (MQ=255)
                                                tATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTAGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCggg                                                                                     >  3:150538/1‑136 (MQ=255)
                                                tATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTAGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCggg                                                                                     >  3:153272/1‑136 (MQ=255)
                                                  tGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCAGCTCCggg                                                                                     >  3:140776/1‑134 (MQ=255)
                                                    gATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGc                                                                                 <  4:8982/136‑1 (MQ=255)
                                                     aTTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGc                                                                                 >  4:133488/1‑135 (MQ=255)
                                                         ggCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGt                                                                             <  8:139852/135‑1 (MQ=255)
                                                         ggCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGt                                                                             <  4:197490/135‑1 (MQ=255)
                                                             tACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAac                                                                         >  2:167924/1‑135 (MQ=255)
                                                                  gACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAg                                                                                                                         <  3:70780/82‑1 (MQ=255)
                                                                  gACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAg                                                                                                                         >  4:70780/1‑82 (MQ=255)
                                                                  gACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTc                                                                   >  6:154531/1‑136 (MQ=255)
                                                                  gACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTc                                                                   >  7:150337/1‑136 (MQ=255)
                                                                   aCCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTc                                                                   <  3:196547/135‑1 (MQ=255)
                                                                      ggCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTc                                                               <  8:150337/136‑1 (MQ=255)
                                                                              aCCGGCTGATGTTGTCACGTTGAGAGCCGCAGTCTGCGACGATAAATTCCTTCAGCGCGCACAGGGCAAGTCATCTTAGAAGCTGGGGATTGAGCTCCGCTCCGGGGTGCATGTTAACCACCTCGGTCTGCTCATa                                                       <  7:181901/136‑1 (MQ=255)
                                                                                cGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGa                                                     <  3:126533/136‑1 (MQ=255)
                                                                                     gATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGcc                                                >  5:25683/1‑136 (MQ=255)
                                                                                         ttCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGtt                                            <  6:87738/136‑1 (MQ=255)
                                                                                                cATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAg                                     >  6:45189/1‑136 (MQ=255)
                                                                                                           cAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAgcg                          >  3:124923/1‑136 (MQ=255)
                                                                                                           cAGACTGCGACGATAAATACCTTCAGGTAGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAgcg                          <  4:153272/136‑1 (MQ=255)
                                                                                                           cAGACTGCGACGATAAATACCTTCAGGTAGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAgcg                          <  4:150538/136‑1 (MQ=255)
                                                                                                                  cgacgaTAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGtt                   <  2:134525/136‑1 (MQ=255)
                                                                                                                  cgacgaTAAATACCTTCAGGTCGCACAGGGCAAGACAGCTAAGAAGCGTCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAgg                    <  4:109861/135‑2 (MQ=255)
                                                                                                                     cgaTAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCgg                                                                                      <  5:165205/66‑1 (MQ=255)
                                                                                                                     cgaTAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCgg                                                                                      >  6:165205/1‑66 (MQ=255)
                                                                                                                     cgaTAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTg                 >  4:119836/1‑135 (MQ=255)
                                                                                                                      gaTAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCa               <  2:97321/136‑1 (MQ=255)
                                                                                                                      gaTAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCAGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCa               <  4:140776/136‑1 (MQ=255)
                                                                                                                        tAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCAAt             <  5:45189/136‑1 (MQ=255)
                                                                                                                                  cAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCAATCCTTATCTaa   >  7:135735/1‑136 (MQ=255)
                                                                                                                                   acgTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCAATCCTTATCTaa   <  5:14/133‑1 (MQ=255)
                                                                                                                                   aGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCAATCCTTATCTaa   <  3:87074/135‑1 (MQ=255)
                                                                                                                                    ggTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCAATCCTTATCTAAt  >  3:172264/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                                   
GATCTGGAACGCCGTTTACGATCGTGCTGCCAGCGAGCTACGCGACGCTATGGATTTGGCCTACCTGACCGGCCAACGACCGGCTGATGTTCTCACCATGAGAGCCGCAGACTGCGACGATAAATACCTTCAGGTCGCACAGGGCAAGACATCTAAGAAGCTGCGGATTGAGCTCCGCTCCGGGGAGCAAGTTAACCACCTCGGTCTGCTCATAGAACGCCTGTTGGCTCAGCGGAAGGAGCGCGGAGTTTGCAATCCTTATCTAAT  >  ACB122/4487727‑4487993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑