| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,759,487 | A→T | 15.4% | intergenic (+85/‑54) | fpvA_3 → / → vanA | Ferripyoverdine receptor/vanillate demethylase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,759,487 | 0 | A | T | 15.4% | 62.4 / 5.5 | 26 | intergenic (+85/‑54) | fpvA_3/vanA | Ferripyoverdine receptor/vanillate demethylase A |
| Reads supporting (aligned to +/- strand): ref base A (9/13); new base T (2/2); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGGAGGACAGCTATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGG > ACB122/4759355‑4759609 | tATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGGTTCTGATCGCCTTAGTTTGAACGCAAAAAACCGCACCGAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAAtgt < 1:130531/136‑1 (MQ=255) cACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTTGAGCTGTTGACAATTAATCATCGGCTCGTTTAAAGTTTGGaaattt > 2:94579/1‑132 (MQ=255) cACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATtgt > 3:129589/1‑136 (MQ=255) cACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATtgt > 8:32129/1‑136 (MQ=255) cACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATtgt > 4:102608/1‑136 (MQ=255) tCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAAtt < 2:160157/136‑1 (MQ=255) tCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGGGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAAtt < 2:35456/136‑1 (MQ=255) aCCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTc < 5:34771/136‑1 (MQ=255) aCCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTc < 1:11419/136‑1 (MQ=255) aCCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTc < 4:111314/136‑1 (MQ=255) cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAgagga > 6:21669/1‑134 (MQ=255) cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAgagga < 3:103212/134‑1 (MQ=255) cTGATCGCCTGACGTTGAACGAAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAgagga < 5:21669/134‑1 (MQ=255) tGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGGGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAggagga < 4:129589/136‑1 (MQ=255) aTCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAggagga < 2:10083/134‑1 (MQ=255) ccTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGGAGGACAGCTa < 3:187319/136‑1 (MQ=255) gaaccaaaaaacCCGCACCCTGGTGCGTTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGGAGGACAGCTATGTAcccc < 1:94579/129‑1 (MQ=255) ggTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGGAGGACAGCTATGTACCCCAAAAACACCTGGTACGTCGCCt < 6:171515/136‑1 (MQ=255) cGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGGAGGACAGCTATGTACCCCAAAAACACCTGGTACGTCGCCTGCAc > 1:165782/1‑136 (MQ=255) ttttttGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGGAGGACAGCTATGTACCCCAAAAACACCTGGTACGTCGCCTGCAcccc > 8:23878/1‑136 (MQ=255) atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGGAGGACAGCTATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAACCCCCTGGGACgg > 6:107749/1‑136 (MQ=255) cTCGTATGTTGTGTGGAATTGTGAGCGGATAACAAttt > 1:133399/1‑38 (MQ=255) cTCGTATGTTGTGTGGAATTGTGAGCGGATAACAAttt < 2:133399/38‑1 (MQ=255) cTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGTAGGACAGCTATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCACACCCCTGGGCCgg > 7:89248/1‑131 (MQ=255) cGTATGTTGTGTGGAATTGTGAGCGGATAACAAttt < 2:123148/36‑1 (MQ=255) cGTATGTTGTGTGGAATTGTGAGCGGATAACAAttt > 1:123148/1‑36 (MQ=255) | TATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACTCTAGAGAGGAGGACAGCTATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGG > ACB122/4759355‑4759609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |