| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,801,948 | G→T | 38.9% | intergenic (‑268/‑463) | CJ019_04509 ← / → dsbD_2 | hypothetical protein/Thiol:disulfide interchange protein DsbD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,801,948 | 0 | G | T | 38.9% | 5.8 / 2.6 | 10 | intergenic (‑268/‑463) | CJ019_04509/dsbD_2 | hypothetical protein/Thiol:disulfide interchange protein DsbD |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base T (2/2); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATG > ACB122/4801829‑4802053 | gggCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCgccgcc > 7:19508/1‑136 (MQ=255) gCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGccgcccg > 1:41100/1‑136 (MQ=255) gCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGAtt > 4:186300/1‑136 (MQ=255) ggATTTTCAGCGCCGGGGAGGTGTTCGCGGGGAGAGTTGCCGCGCCTGGGAGAGCGCGCGCCGCCCGCGGGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTgcgcg < 4:196488/136‑1 (MQ=21) gtGGGGGGGGTGCGCGGGGTGCGTTTCCGCGCGTGGGAGAGCGCGCGCCCCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTggg < 8:19508/135‑1 (MQ=18) aGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATg < 3:186300/136‑1 (MQ=255) ccTGTGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAattat < 7:130384/74‑1 (MQ=14) ccTGTGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAattat > 8:130384/1‑74 (MQ=14) ccTGTGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGATAAACCCGCTCCTACGTTTGTTTCGGGCCAattat < 7:120330/74‑1 (MQ=9) ccTGTGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGATAAACCCGCTCCTACGTTTGTTTCGGGCCAattat > 8:120330/1‑74 (MQ=9) | GGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATG > ACB122/4801829‑4802053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |