Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,950,614 G→A 10.8% R30C (CGC→TGC)  fliI ← Flagellum‑specific ATP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,950,6140GA10.8% 104.4 / 3.7 37R30C (CGC→TGC) fliIFlagellum‑specific ATP synthase
Reads supporting (aligned to +/- strand):  ref base G (19/14);  new base A (2/2);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACGCTCGGCCGGCGCGTCCAGGTCTATCGA  >  ACB122/4950503‑4950747
                                                                                                               |                                                                                                                                     
ccTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCgg                                                                                                               >  4:201986/1‑136 (MQ=255)
ccTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCgg                                                                                                               >  3:69041/1‑136 (MQ=255)
        tGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGa                                                                                                       >  7:24261/1‑136 (MQ=255)
           aCCGGGTGATAACTGGCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGGGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATgg                                                                                                    <  7:166624/136‑1 (MQ=255)
                  gATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTcg                                                                                                                                <  4:131010/101‑1 (MQ=255)
                  gATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTcg                                                                                                                                >  3:131010/1‑101 (MQ=255)
                  gATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGc                                                                                             >  8:83136/1‑136 (MQ=255)
                  gATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGc                                                                                             >  8:186673/1‑136 (MQ=255)
                  gATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGc                                                                                             >  1:205609/1‑136 (MQ=255)
                    tAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGt                                                                                           <  5:196576/136‑1 (MQ=255)
                              cggggATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGGGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAg                                                                                 <  5:36475/132‑1 (MQ=255)
                               gTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGa                                                                                <  8:24261/136‑1 (MQ=255)
                                            cAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGc                                                                   <  6:31174/136‑1 (MQ=255)
                                              gCGGCTGCCCACTGCGGCGCGCAGGCCTGCGGCGTCCAGGGTGAGGCCGAGCATACGCAGCAAGCGGCCGTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTg                                                                 <  3:114498/136‑1 (MQ=255)
                                                       cACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCa                                                        <  3:192958/136‑1 (MQ=255)
                                                       cACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCa                                                        <  4:88596/136‑1 (MQ=255)
                                                         ctgctgCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAg                                                      <  7:83136/136‑1 (MQ=255)
                                                             tgcGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGgcg                                                   >  4:35873/1‑135 (MQ=255)
                                                                 cgcAGGCCTTCGGCGTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCt                                              <  2:178901/136‑1 (MQ=255)
                                                                   cAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTgg                                            >  5:75956/1‑136 (MQ=255)
                                                                    gggCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCAGCCTTCGGCGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGGCGCAGCTGGTGCGTTGAAGGCGCATCTggg                                           >  1:111338/2‑136 (MQ=255)
                                                                    aGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCAGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGCAGCTGGTGCGTTCAAGGCGCATCTggg                                           >  1:108078/1‑136 (MQ=255)
                                                                       ccTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTg                                        >  3:15485/1‑136 (MQ=255)
                                                                       ccTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTAGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTg                                        >  7:86972/1‑136 (MQ=255)
                                                                              cTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACgg                                 >  1:148899/1‑136 (MQ=255)
                                                                                   aGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCt                                                                            <  4:170860/88‑1 (MQ=255)
                                                                                   aGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCt                                                                            >  3:170860/1‑88 (MQ=255)
                                                                                   aGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACg                             >  5:138411/1‑135 (MQ=255)
                                                                                   aGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACGc                            >  4:131440/1‑136 (MQ=255)
                                                                                   aGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACGc                            >  7:24801/1‑136 (MQ=255)
                                                                                              gACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACGCTCGGCCGgcgc                 <  7:24568/136‑1 (MQ=255)
                                                                                                ccATAGGCAGCAAGCAGCCTTCGACGATGGGCTGGGCCGGCAGCTGGATGGCCTCTGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCACGGCGCTTCTGGGCTGTCCACGGCACGCTCGGCCGGCGCGt               <  2:111338/136‑1 (MQ=255)
                                                                                                ccATACGCAGCAAGCAGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACGCTCGGCCGGCTCGt               <  2:108078/136‑1 (MQ=255)
                                                                                                  aTACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACGCTcggc                       <  1:129260/126‑1 (MQ=255)
                                                                                                  aTACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACGCTcggc                       >  2:129260/1‑126 (MQ=255)
                                                                                                            aGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTAAAGGCGCATCTGGGCTGTCCACGGCACGCTCGGCCGGCGCGTCCCGGTCTATCg   >  5:89725/1‑136 (MQ=255)
                                                                                                             gCGGCCTTCGACGATGGGCTGGGCCGACAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCCTCTGGGCTGTCAACGGCACGCTCGGCCGGCGCGAGCAGGTGGATCGa  >  5:125496/1‑136 (MQ=255)
                                                                                                               |                                                                                                                                     
CCTCGACCTGCACCGGGTGATAACTGTCATCGTTGATCACCAGGCAGCGGCTGCCCACTGCTGCGCGCAGGCCTTCGGCTTCCAGGGTGAGGCCGACCATACGCAGCAAGCGGCCTTCGACGATGGGCTGGGCCGGCAGCTCGATGGCCTCAGCGTAAGTGCCCAGACGCTTGCCGAAGCTGGTGCGTTCAAGGCGCATCTGGGCTGTCCACGGCACGCTCGGCCGGCGCGTCCAGGTCTATCGA  >  ACB122/4950503‑4950747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑