| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,094,168 | A→C | 28.7% | F476C (TTT→TGT) | tyrR_2 ← | Transcriptional regulatory protein TyrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,094,168 | 0 | A | C | 28.7% | 12.2 / 7.5 | 14 | F476C (TTT→TGT) | tyrR_2 | Transcriptional regulatory protein TyrR |
| Reads supporting (aligned to +/- strand): ref base A (7/3); new base C (2/2); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 | |||||||||||
TCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATCCGGCAAACGAATATGCTCGCTTTTAACCACGCCGCCG > ACB122/5094063‑5094275 | tCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCtt > 5:70282/1‑136 (MQ=255) ggCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAACACTCg > 8:93581/1‑136 (MQ=255) gCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGCCTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCg > 3:186477/1‑136 (MQ=255) gTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATc > 3:116750/1‑136 (MQ=255) gCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATCCGGCAAACGAatat < 3:45832/136‑1 (MQ=255) ccGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCACAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGcc < 1:86304/107‑1 (MQ=255) ccGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCACAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGcc < 1:88970/107‑1 (MQ=255) ccGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCACAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGcc > 2:86304/1‑107 (MQ=255) ccGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCACAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGcc > 2:88970/1‑107 (MQ=255) gAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATCCGGCAAACGAATATGCTCGCGTTTAAc > 6:174795/1‑136 (MQ=255) aaaCTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATCCGGCAAACGAATATGCTCGCTTTTAAcc < 7:93581/136‑1 (MQ=255) aaCTCCCCCATCAAACTTTCCAGAACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATCCGGCAAACGAATATGCTCGCTTTTAACCa < 1:145579/136‑1 (MQ=255) cATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATCCGGCAAACGAATATGCTCGCTTTTAACCACGccgccg > 3:170847/1‑136 (MQ=255) cATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATCCGGCAAACGAATATGCTCGCTTTTAACCACGccgccg > 7:172618/1‑136 (MQ=255) | TCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAGCACCGCCTTTTCAAAGCGCCCGACTATCTGCGAAAGATCCCCTTCCAGCGAAAACTCGCCCAACGGTTGCCGCACGCCATAATCCGGCAAACGAATATGCTCGCTTTTAACCACGCCGCCG > ACB122/5094063‑5094275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |