| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 150,804 | A→C | 48.2% | W7G (TGG→GGG) | CJ019_00156 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 150,804 | 0 | A | C | 48.2% | 1.6 / 7.8 | 14 | W7G (TGG→GGG) | CJ019_00156 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base C (4/3); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01 | |||||||||||
TCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTC > ACB122/150683‑150929 | tcgtcgAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCGCAAGCTGCAGGGCGGCCCAGGCGGGCGTGCCGCGCAGCGGCCGGGCGCCGCCCAGCAGCCCGGCACCCACgcgcg > 3:167494/1‑135 (MQ=255) ttAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCACCTCAAGCTGGACGTGGGCCAACGCGGGCGTGCCCACCAGCGGCCCGGCGCCGCCCACCAGCCCCGCCCCCACCCCCGTGACAATGCGAGTATTGCttaacc > 4:66271/1‑132 (MQ=255) tAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAACCGGCAGGTCGGCCAACGCGGGCGTGCCGCGCCGCGTCACGGCGCCGCCCAGCAGCCCCGCCCCCACCCGCTTCACAGTGCGTGTATGGCGTCAGcc > 6:97150/1‑136 (MQ=255) cGAGGCCGGACGCCTGCACCGCAAGCAGCAGCTGGGCCAAGGCGGGCGGGCCGCGCAGCGGCAGGGCGCAGCCAAGCACCCCCGCACGCCCGCGGGTAACCCTGGGCGTATGGGTTAACCCAAAACCAGGTCgggg > 4:168461/1‑133 (MQ=255) ggccggTGGGCCAAGGGGGGGGGGCCGAGCAGGGTCAGGGCGCCGCCAAGCAGCCCGGCACGAACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctc < 2:32456/132‑1 (MQ=255) gcacgctCGCCCAAGGCGGGCGTGCCGAGCCGCGTCAGGGCGCAGCCAACCCCCCCGCAACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctc < 7:126180/130‑1 (MQ=255) ctgggcGGCCATGGGGGGCGGCCCGTCCTGCGTCAGGGCCCAGCCAAGCAGCCAGCCACGCAGCCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTActcgctc < 2:49806/129‑1 (MQ=255) cggggcGGACAAGGCGGGCGTGCCGAGCCGGGTCAGGGCGCTGCCAAGCAGCCAGGACCGAACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACtcgctcgc < 4:9417/131‑1 (MQ=255) cgggTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCTGGGCGCAGCAAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACtcgctcgc < 3:66271/134‑1 (MQ=255) gtgcGGCAATGGCGGGCGGCCGGAGCCGCGGCAGGGCGCAGCAAAGCAGCCTGGCACGAACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCgcgc < 2:1168/133‑1 (MQ=255) gggcGGCCAAGGCGGGCGTGCCGCGCAGCGGCAGGGCGCAGCCAAGCAGCCAGGCACGAACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCgcgc < 6:144920/133‑1 (MQ=255) gCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCt > 2:185622/1‑136 (MQ=255) tctgggCGCAGCCAAGCAGCCCGGCACGCACGCGCTTCACAGTGCGAGTAGGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTAcg < 3:158330/133‑1 (MQ=255) ccAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCGCGCGTTGTACGCCGCGGCTGTc > 1:69349/1‑136 (MQ=255) | TCGTCGAGCAGGGCTTGGGAGGCTTTAGTTTGTTGTGGGTCGAGGCCGGACGCCTGCAGCTCAAGCTGCAGGTCGGCCAAGGCGGGCGTGCCGAGCAGCGTCAGGGCGCAGCCAAGCAGCCAGGCACGCACGCGCTTCACAGTGCGAGTATGGCTTCAGCCAATACCTGGTCGCTGGCGCCGCGCGCTTCCGGTACTCGCTCGCGCAGGGTATTGAAGGCCGCCTCGAGTTGTACGCCGCGGATGTC > ACB122/150683‑150929 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |