Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,644,969 C→A 8.4% A51S (GCC→TCC)  cbbA ← Fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,644,9690CA 8.4% 116.0 / 4.1 48A51S (GCC→TCC) cbbAFructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (29/15);  new base A (2/2);  total (31/17)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

GGATCGAGCGCTGGCAGACGTCAGGGCTGGTACCGTGGTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTCCAGCATCTGGCGC  >  ACB122/5644834‑5645102
                                                                                                                                       |                                                                                                                                     
ggATCGAGCGCTGGCAGACGTCAGGGCTGGTACCGTGGTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGc                                                                                                                                       >  3:49503/1‑136 (MQ=255)
         gcTGGCAGACGTCAGGGCTGGTACCGTGGTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCt                                                                                                                               >  7:127325/1‑135 (MQ=255)
            ggCAGACGTCAGGGCTGGTACCGTGGTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAAc                                                                                                                           <  3:81373/136‑1 (MQ=255)
               aGACGTCAGGGCTGGTACCGTGGTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGAt                                                                                                                        >  6:117057/1‑136 (MQ=255)
                    tCAGGGCTGGTACCGTGGTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCg                                                                                                                   <  3:122959/136‑1 (MQ=255)
                                ccGTGGTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCt                                                                                                       >  7:123798/1‑136 (MQ=255)
                                     gTCCTGGTGCATGCACACCGGGATGTGCGGGACCTCTTCGATGGCAGCCAGGATCAGGTGGAGCAGGAATGGCTCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGTTCTTGTCg                                                                                                  >  4:190449/1‑136 (MQ=255)
                                     gTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTc                                                                                                   <  8:127325/135‑1 (MQ=255)
                                     gTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCg                                                                                                  >  1:18339/1‑136 (MQ=255)
                                      tCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCgg                                                                                                 >  3:42851/1‑136 (MQ=255)
                                      tCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGAAGCCAGGATCAGGTGGCGCTGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGACCGATCACCGGGGAGTCGGTCTTGTCgg                                                                                                 >  6:183052/1‑136 (MQ=255)
                                                 gcaCACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCTCAACCGCGTATTTGCGGGCACCGGCAGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCAat                                                                                       >  3:134257/1‑135 (MQ=255)
                                                  cacaCCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCatga                                                                                     >  3:160186/1‑136 (MQ=255)
                                                   acacCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCatgat                                                                                    >  2:60317/1‑136 (MQ=255)
                                                   acacCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCatgat                                                                                    >  2:65084/1‑136 (MQ=255)
                                                   acacCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCatgat                                                                                    >  1:181175/1‑136 (MQ=255)
                                                   acacCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCGTCCatgat                                                                                    >  1:142439/1‑136 (MQ=255)
                                                   acacCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGACGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCatgat                                                                                    >  8:190840/1‑136 (MQ=255)
                                                   acacCGGGATGTGCGGGAACTCTTCGAAGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGACGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCGTCCatgat                                                                                    >  2:128429/1‑136 (MQ=255)
                                                     acCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGACCGATCACCGGGGCGTCGGTCTTGTCGGCCGCTTCCatgattg                                                                                  >  7:193208/1‑134 (MQ=255)
                                                                 gggAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCAgctgct                                                                      >  6:191784/1‑136 (MQ=255)
                                                                  ggAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTc                                                                                                   >  1:67710/1‑106 (MQ=255)
                                                                  ggAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTc                                                                                                   <  2:67710/106‑1 (MQ=255)
                                                                  ggAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTc                                                                     >  8:159042/1‑136 (MQ=255)
                                                                  ggAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTc                                                                     <  8:123798/136‑1 (MQ=255)
                                                                    aaCTCTTCGATGGCAGCCAGGATCAGGCGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTc                                                                                                   <  4:176415/104‑1 (MQ=255)
                                                                    aaCTCTTCGATGGCAGCCAGGATCAGGAGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTc                                                                                                   >  3:176415/1‑104 (MQ=255)
                                                                                 cAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGtt                                                      <  3:86014/136‑1 (MQ=255)
                                                                                                ggCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGcc                                       >  1:34129/1‑136 (MQ=255)
                                                                                                 gcgcAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCg                                      >  3:29825/1‑136 (MQ=255)
                                                                                                     aGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCGCGAGGTTGTTGGCGTTGAAAGCTGGTACGCCGTAGc                                  >  1:79908/1‑136 (MQ=255)
                                                                                                               cACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTcggc                        >  6:90729/1‑136 (MQ=255)
                                                                                                                aCCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTcggcg                       <  4:121964/136‑1 (MQ=255)
                                                                                                                  ccGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGGCTTGTCGGCCGCTTCCATGATGGCGCGGAGGTGCTCGAGGTTGGTGACGGTGAAAGCTGGTACGCCGTAGCCGCACGCggcggc                     >  6:125739/1‑136 (MQ=255)
                                                                                                                  ccGCGTATTTGCGGGCACCGGACGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCggcggc                     <  7:190840/136‑1 (MQ=255)
                                                                                                                  ccGCGTATTTGCGGGCACCGGACGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGGTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCggcggc                     <  1:128429/136‑1 (MQ=255)
                                                                                                                    gcgTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGt                   >  7:66742/1‑136 (MQ=255)
                                                                                                                    gcgTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCGTCCATGATGGCGCGCCTCTGCGCGAGGTTGTTTACGTTGTAAGCTGGTACGCCGTAGCCGCAATAGgcggcgg                   >  5:108073/1‑135 (MQ=255)
                                                                                                                     cgTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGGTGTTGACGTTGAAAGCTGGTAAGCCGTAGCCGAACTCGGCGGCGTg                  >  8:18628/1‑136 (MQ=255)
                                                                                                                               ggCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGCACTCGGCGGCGGGGTCCCGCATc        >  6:161174/1‑136 (MQ=255)
                                                                                                                                 cACCGGCCGTAGGCTGAACGATCTCCGGGGAGTCGGTCTTGTCGGCCGCTTCGATGATGGCGCGCATCTGCTGGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGGGGCGTGGTCCAGCATCTg      <  3:161128/136‑1 (MQ=255)
                                                                                                                                 cACCGGCCGAAGCCTGAACGCTCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTCCAGCATCTg      <  2:172805/136‑1 (MQ=255)
                                                                                                                                 cACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTCCAGCATCTg      <  1:120854/136‑1 (MQ=255)
                                                                                                                                 cACCGGCCGAAGCCGGAACGATCCCCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCAGCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTCCAGCATCTg      <  6:129649/136‑1 (MQ=255)
                                                                                                                                  aCCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCGGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTGCAGCATCTgg     >  5:40582/1‑136 (MQ=255)
                                                                                                                                   ccGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTCCAGCATCTGgc    <  5:117057/136‑1 (MQ=255)
                                                                                                                                     ggCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGGGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGAGGCGTGGTCCAGCATCTGgcgg  >  6:159090/1‑135 (MQ=255)
                                                                                                                                     ggCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTCCAGCATCTGgcgc  <  5:90729/136‑1 (MQ=255)
                                                                                                                                     ggCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTCCAGCATCTGgcgc  <  7:19852/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GGATCGAGCGCTGGCAGACGTCAGGGCTGGTACCGTGGTCCTGGTGCATGCACACCGGGATGTGCGGGAACTCTTCGATGGCAGCCAGGATCAGGTGGCGCAGGAATGGCGCACCCGCGTATTTGCGGGCACCGGCCGAAGCCTGAACGATCACCGGGGAGTCGGTCTTGTCGGCCGCTTCCATGATGGCGCGCATCTGCTCGAGGTTGTTGACGTTGAAAGCTGGTACGCCGTAGCCGAACTCGGCGGCGTGGTCCAGCATCTGGCGC  >  ACB122/5644834‑5645102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑