| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 6,095,820 | A→T | 15.9% | intergenic (+129/‑4) | uvrD_2 → / → CJ019_05710 | DNA helicase II/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 6,095,820 | 0 | A | T | 15.9% | 51.2 / 2.7 | 24 | intergenic (+129/‑4) | uvrD_2/CJ019_05710 | DNA helicase II/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (9/11); new base T (2/2); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGGCCTGCCAACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACG > ACB122/6095705‑6095955 | cGGGCCTGCCAACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAg < 2:140112/136‑1 (MQ=255) cTTTGTAACATTCAAGCAACAGCTCTCAACCCTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCTATCCACAACAGGTTATATCCCACTTCTGCAACGTTTTCTTAGCATCGCACTGgc < 5:114007/136‑1 (MQ=255) aCATTCAAGCAAACGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTg < 2:62241/135‑1 (MQ=255) aTTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGACCCTGTGCACCATGCCGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGt > 7:51541/1‑136 (MQ=255) cAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCg < 6:199359/135‑1 (MQ=255) aGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGcc > 2:146401/1‑136 (MQ=255) aaaaGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGa < 4:46017/136‑1 (MQ=255) cTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGAGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCt > 2:5557/1‑135 (MQ=255) gtgCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGtt > 5:34621/1‑136 (MQ=255) gtgCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAag > 7:121072/1‑136 (MQ=255) cACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAg > 6:23695/1‑60 (MQ=255) cACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAg < 5:23695/60‑1 (MQ=255) tGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCGGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCggg < 5:155924/136‑1 (MQ=255) gACGCGCGTGCAATCCACAACAGGGGATATCCCTCTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGc > 5:123708/1‑136 (MQ=255) gACGCGCGTGCAATCCACAACAGGGGATATCCCTCTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGc > 5:121232/1‑136 (MQ=255) gagagaTCCACAACAGGGGATATCCCACTTATGCAACGCCTTCTTATCATCGCACTGGCGCTCTGCGTCGGGCTGACGCTGAGCCTGGACGCCAGCGACAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCgcgc < 6:170526/131‑1 (MQ=255) cacaACAGGGGATATCCCTCTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccac < 6:121232/136‑1 (MQ=255) cacaACAGGGGATATCCCTCTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATccac < 6:123708/136‑1 (MQ=255) aGGGGATATCCCACTTATGCAACGTTTTCTTATCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGAcc < 4:15147/136‑1 (MQ=255) aGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGAcc < 6:34621/136‑1 (MQ=255) gATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGcc > 1:23595/1‑136 (MQ=255) atatCCCACTTATGCAACGTTTTCTTAGCATCTCACTGGCGCTCTTCGTCGGCCTGACGCTGAGCCTGCACGCCAGCCCCAAGAGTTTCGGCGGCTGCAAGAGCTCTTTCTCCGCGCCTATCCACCAGAcactctc > 5:170526/1‑130 (MQ=255) atatCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGc > 5:130393/1‑134 (MQ=255) ccACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTTTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCt < 3:25786/135‑1 (MQ=255) aCTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACg > 7:198841/1‑136 (MQ=255) | CGGGCCTGCCAACTTTGTAACATTCAAGCAAAAGCTCGAAACACTATGTCGCTGGTCATGTGCCCGGGAACCTGTGCACCATGACGCGCGTGCAATCCACAACAGGGGATATCCCACTTATGCAACGTTTTCTTAGCATCGCACTGGCGCTCTGCGTCGGCCTGACGCTGAGCCTGGACGCCAGCGCCAAGCGTTTCGGCGGCGGCAAGAGCTCGGGCTCCGCGCCTATCCACCAGACCCGCCAGGCTACG > ACB122/6095705‑6095955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |