| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 172,649 | C→T | 8.2% | intergenic (+3613/+74) | PP_RS08750 → / ← fhuA_1 | tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 172,649 | 0 | C | T | 8.2% | 146.0 / 3.8 | 49 | intergenic (+3613/+74) | PP_RS08750/fhuA_1 | tRNA‑Val/Ferrichrome outer membrane transporter/phagereceptor |
| Reads supporting (aligned to +/- strand): ref base C (25/20); new base T (2/2); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.02e-01 | |||||||||||
GCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTT > ACB122/172524‑172782 | gCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGAtt > 5:203398/1‑135 (MQ=39) ccAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTc > 4:66451/1‑135 (MQ=38) cAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATTGTCAAGATTCa > 2:38443/1‑135 (MQ=37) cAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATTGTCAAGATTCa > 2:38439/1‑135 (MQ=37) cAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCa > 8:91272/1‑135 (MQ=37) cacaGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCa < 8:12439/136‑1 (MQ=38) aTTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCa > 1:156420/1‑130 (MQ=38) aTTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCa < 2:156420/130‑1 (MQ=38) cTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTg < 1:163599/135‑1 (MQ=255) gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGt < 4:133228/133‑1 (MQ=255) ccATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATg > 2:120011/1‑136 (MQ=255) tGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCACCCGT‑CGGCGGTGCAGACAGGGGc > 5:11261/1‑136 (MQ=255) aaCCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTAATCGTCAAGATTCAAACCCAAAGCCCCAGT‑CTGCGATGCAGACAGGGGCttt > 7:6666/1‑136 (MQ=255) aaCCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCACCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCttt > 5:107864/1‑136 (MQ=255) tGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCtttt > 1:141945/1‑136 (MQ=255) tGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCtttt > 4:17306/1‑136 (MQ=255) tGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCtttt > 1:40663/1‑136 (MQ=255) tGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCAGTGATGC‑AAGCACACAGGGGCATTGttgttt > 1:187397/1‑131 (MQ=14) ggagcccatcccGAACTCAGCAGTGAAACGAGGCATCGCCGATGGTCGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCtttt < 4:200543/132‑1 (MQ=255) atcccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATTGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCtttt < 1:38439/134‑1 (MQ=255) atcccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATTGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCtttt < 1:38443/134‑1 (MQ=255) ccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTc > 4:47663/1‑135 (MQ=255) cGAACTCAGCAGTGGAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCaa > 6:174103/1‑136 (MQ=255) agTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGt > 7:70713/1‑133 (MQ=255) agTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTACGGGTTTCAATATCGGt > 5:179958/1‑133 (MQ=255) gATGCATCCCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATa < 6:107864/136‑1 (MQ=255) gccttgggTTGTGTGGGGTTGCCCCTTGTGCGATTAGGGCATCGCCAAGATTCAACCCCAACCCCCCTGT‑CTGCGAGCCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGAc < 7:190491/128‑1 (MQ=255) gATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAACGCCCCTGT‑CTGCTATGCAGACTGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTgg < 1:43272/136‑1 (MQ=255) tgtgtgGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTcaca < 4:48457/136‑1 (MQ=255) tgtgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTcaca < 8:189731/136‑1 (MQ=255) tgtgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTcaca < 3:17306/136‑1 (MQ=255) ggggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTg < 2:184100/55‑1 (MQ=255) ggggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTg > 1:184100/1‑55 (MQ=255) ggggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTg > 5:163986/1‑136 (MQ=255) ccccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGctct > 6:158403/1‑135 (MQ=255) ccccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCGGT‑CTGCGATGCAGACAGGGGGGTTGTGGTTTGCGGGGTTGAATATCGGTTAGAAAGGATAGCTGCCCGTGGCGGTCACATTgggcgatt > 8:76376/1‑132 (MQ=25) ccATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCa > 6:185832/1‑136 (MQ=255) cATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCAc < 8:42281/136‑1 (MQ=255) gagaGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATg > 2:33549/1‑136 (MQ=255) gagTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTa < 2:40663/136‑1 (MQ=255) agTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAg < 3:47663/136‑1 (MQ=255) agTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAg < 2:141945/136‑1 (MQ=255) aGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAg > 7:53032/1‑133 (MQ=255) aGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAg < 8:53032/133‑1 (MQ=255) ggaaTCGTCAAGATTCAAACCCAAAGCCCCTGG‑CTGCGATCCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGGGGCGCTCACATTGCGCTCTTCACCCATGTAGCagta < 3:82750/133‑1 (MQ=255) gTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCagta < 8:70713/136‑1 (MQ=255) cATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTa > 3:70336/1‑128 (MQ=255) cATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTa < 4:70336/128‑1 (MQ=255) aTCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGtt > 4:183001/1‑136 (MQ=255) | GCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGT‑CTGCGATGCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTT > ACB122/172524‑172782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |