| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 523,499 | G→T | 13.9% | W121C (TGG→TGT) | birA → | Bifunctional ligase/repressor BirA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 523,499 | 0 | G | T | 13.9% | 62.9 / 5.6 | 29 | W121C (TGG→TGT) | birA | Bifunctional ligase/repressor BirA |
| Reads supporting (aligned to +/- strand): ref base G (13/12); new base T (2/2); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01 | |||||||||||
GCCGGTATTCATCCACGAAACCATCGATTCGACCAATGCCGAAGGCTTGCGCCTTGCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGCTCGAGGGGTTGAGCTTGGTAGTGGGGTTGGCGGTAATGCGCACCTTGCAGGCGTTCGGTGTAAAGGA > ACB122/523364‑523633 | gCCGGTATTCATCCACGAAACCATCGATTCGACCAATGCCGAAGGCTTGCGCCTTGCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTgg < 6:75172/136‑1 (MQ=255) ccccGAAACCATCGATGCGACCAATGCCGACGGCTTGCGCCTTGCCAGTCACGGGCAGGCGGCTCCATTCCTGGTGCTGGCTGCGCGCCAGAGCGCCGGACGCGGGCGGCGCGGCCGGCAGTGGGTCAGCCCAttt < 4:42593/133‑1 (MQ=255) aCGAAACCATCGATTCGACCAATGCCGAAGGCTTGCGCCTTGCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGc > 4:122141/1‑136 (MQ=255) caaaCCATGGATTCGACCAATGCCGAAGGCTTGCGCCTGCCCAGGCAAGGTCAGGCGGCTCCATTCCTGGTGCTGGCTGAGCGCCAGAGCGCCGGAGGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTg < 4:163311/135‑1 (MQ=255) aaCCATCGATTCGACCAATGCCGAAGGCTTGCGCCTTGCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGa > 2:38135/1‑135 (MQ=255) aTCGATTCGACCAATGCCGAAGGCTTGCGCCTTGCCAGTGAAGGGCAGGCGGCTCCAATCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAAt > 2:47726/1‑135 (MQ=255) cgcCTTGCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTc > 4:9695/1‑135 (MQ=255) cgcCTTGCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGGCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCg < 3:181343/136‑1 (MQ=255) gcCTTGCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGa > 1:140400/1‑136 (MQ=255) cTTGCCAGTGACGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATg < 6:77817/136‑1 (MQ=255) gCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGGGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGa < 1:2228/135‑1 (MQ=255) cAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGCCGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGc > 8:192485/1‑136 (MQ=255) cAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAgg > 1:78370/1‑135 (MQ=255) aaGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCg > 2:172084/1‑136 (MQ=255) cAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTTGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGc < 5:137203/136‑1 (MQ=255) aGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGcc < 8:18481/75‑1 (MQ=255) aGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGcc > 7:18481/1‑75 (MQ=255) ggcTCCTTTCCTGGTTCTGGCGGAGCGCCAGAGCGCCGGACGCGGGCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGCTCGAg < 8:86171/136‑1 (MQ=255) ggcGCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGGCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGCTCGAg < 2:70966/136‑1 (MQ=255) aTTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGCTCGAGGGGtt > 8:72116/1‑135 (MQ=255) tCTGGCTGAGCTCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGGCGATGGAGGCATGCGTCAGCTCGAGGGGTTGAGCTTgg > 3:30632/1‑134 (MQ=255) gCTGAGCGCCAGAGCGCCGGCCGCGGGCGGCGCGGCCGGCAGTGGGGCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGCTCGAGGGGTTGAGCTTGGTAGt < 4:3954/134‑1 (MQ=255) gcCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGTGTCAGCCCATTTGCTg < 6:198501/54‑1 (MQ=255) gcCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGTGTCAGCCCATTTGCTg < 6:201236/54‑1 (MQ=255) gcCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGTGTCAGCCCATTTGCTg > 5:201236/1‑54 (MQ=255) gcCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGTGTCAGCCCATTTGCTg > 5:198501/1‑54 (MQ=255) ccAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATg < 5:24599/97‑1 (MQ=255) ccAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATg > 6:24599/1‑97 (MQ=255) ggggcgGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGCTCGAGGGGTTGAGCTTGGTAGTGGGGTTGGCGGTAATGCGCACCTTGCAGGc < 1:47726/133‑1 (MQ=255) ggccggcAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACCGCCTGGTCCCGCGCGTCGATGGAGGCATGCGTCAGCTCGAGGGGTTGAGCTTGGTAGTGGGGTTGGCGGTAATGCTCACCTTGCAGGCGTTCg > 2:123936/1‑136 (MQ=255) gggTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGCTCGAGGGGTTGAGCTTGGTAGTGGGGTTGGCGGTAATGCGCACCTTGCAGGCGTTCGGTGTAAAGGa < 7:192485/136‑1 (MQ=255) | GCCGGTATTCATCCACGAAACCATCGATTCGACCAATGCCGAAGGCTTGCGCCTTGCCAGTGAAGGGCAGGCGGCTCCATTCCTGGTTCTGGCTGAGCGCCAGAGCGCCGGACGCGGTCGGCGCGGCCGGCAGTGGGTCAGCCCATTTGCTGAAAATCTCTATTACAGCCTGGTCCTGCGCGTCGATGGAGGCATGCGTCAGCTCGAGGGGTTGAGCTTGGTAGTGGGGTTGGCGGTAATGCGCACCTTGCAGGCGTTCGGTGTAAAGGA > ACB122/523364‑523633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |