| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,115,355 | T→C | 6.1% | C344R (TGC→CGC) | trkI_2 → | Trk system potassium uptake protein TrkI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,115,355 | 0 | T | C | 6.1% | 203.4 / 2.5 | 65 | C344R (TGC→CGC) | trkI_2 | Trk system potassium uptake protein TrkI |
| Reads supporting (aligned to +/- strand): ref base T (38/23); new base C (2/2); total (40/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATGCCCTGCGCCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACGGCCATCGCCTCGA > ACB122/5115225‑5115488 | cGATGCCCTGCGCCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCCGCTc > 8:64723/1‑135 (MQ=255)cGATGCCCTGCGCCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGGTCTTCTACCTGGGCTTCGTTGGCGGCCGCTc > 8:58766/1‑135 (MQ=255)cGATGCCCTGCGCCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCTTCGCCTTGGGTGACTACAGCCTGTGGGGTTACTTCTCGCTGATCCTGTTCTTCTACCTGGGCGTCGTTGGCGGCTGCTc > 2:149491/1‑135 (MQ=255) aTGCCCTGCGCCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCg < 5:101064/135‑1 (MQ=255) aTGCCCTGCGCCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGATTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGTGCTTCGTTGGCGGCTGCTCCg < 2:369736/135‑1 (MQ=255) cccTGCGCCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCGTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCt > 5:131706/1‑135 (MQ=255) gcCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCCCCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGCCCg > 3:188065/1‑135 (MQ=255) gcCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCg > 3:50259/1‑135 (MQ=255) gcCATGTGGCGCTGAACGTGACCTCGGAGGTCACCACCACCGGCTTCTCCTTGGGGGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTTCCTGGGCTTCGGTGGCGGCTGCTCCGGCTCGAcc > 2:279965/1‑134 (MQ=255) ggCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAggcg > 3:303532/1‑134 (MQ=255) gcgcTGAACGGGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTCCCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGCCCGCAggcggc > 4:73056/1‑135 (MQ=255) tGAACGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGGAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCAt > 3:259980/1‑133 (MQ=255) aCGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTTACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGa > 8:15970/1‑135 (MQ=255) gTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGAtt > 2:117544/1‑135 (MQ=255) gTGACCTCGGTGGTCACCACCACCGGCATCTCCTAGGGTGACTACAGCCTGTGGGCTAAGTTCTCGCTGATGCTGATCATCTAACTGGGCTTCGATGGCGGCTGCTCACCCTCGACCGCCGCCGGCATCAAGAtt > 4:127994/1‑135 (MQ=255) gACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGAtttt < 6:39387/135‑1 (MQ=255) tggtCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCtt > 5:39295/1‑133 (MQ=255) tggtCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTcc < 1:133333/135‑1 (MQ=255) ccaccaccGGCTTCTCCTTGGGTGACTTCAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTgg < 4:303532/135‑1 (MQ=255) ccaccaccGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCTACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTgg < 6:269358/135‑1 (MQ=255) accGGCTTCTCCTTGGGTGACTACAGCCTGCGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATAc < 7:121193/135‑1 (MQ=255) tcCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGtt > 3:167709/1‑134 (MQ=255) tcCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGtt > 7:52424/1‑134 (MQ=255) cTTGGGGGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGaa < 2:352554/135‑1 (MQ=255) ttGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGaa < 7:241024/134‑1 (MQ=255) ttGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAg > 3:153211/1‑135 (MQ=255) aCTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGcc < 1:294760/135‑1 (MQ=255) aCAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGaa > 7:119830/1‑124 (MQ=255) aCAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGaa < 8:119830/124‑1 (MQ=255) aCAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCa > 8:213938/1‑135 (MQ=255) aCAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCa > 2:54728/1‑135 (MQ=255) aCAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCa > 2:271936/1‑135 (MQ=255) aCAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGGTGGCGGCTGCTCCGGCTCGCCCCCACGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCa > 2:266973/1‑135 (MQ=255) aGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCaa > 4:191367/1‑134 (MQ=255) aGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAAc > 1:296608/1‑135 (MQ=255) gggTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATAc > 7:27475/1‑103 (MQ=255) gggTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATAc < 8:27475/103‑1 (MQ=255) gttAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTAGTTGGCTTCTGCTACTGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGAt > 5:71114/3‑134 (MQ=255) ggTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGAt > 4:185338/1‑134 (MQ=255) ctcGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGc > 8:114783/1‑57 (MQ=255) ctcGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGc < 7:114783/57‑1 (MQ=255) cGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCcgcgcg > 5:209978/1‑135 (MQ=255) tGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGt < 4:50259/133‑1 (MQ=255) tGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGa < 2:171957/135‑1 (MQ=255) cTGTTCTTCTACCGGGGCTGCGTTGGCGGCTGCTCCGGAGCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGGGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATc < 2:193263/135‑1 (MQ=255) tGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGaa < 7:17835/94‑1 (MQ=255) tGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGaa > 8:17835/1‑94 (MQ=255) tGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGAt > 7:109063/1‑133 (MQ=255) tGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATc < 6:209978/134‑1 (MQ=255) tGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATc < 3:62008/134‑1 (MQ=255) tcttctACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCaa > 5:274635/1‑133 (MQ=255) tcttctACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGc < 1:117544/135‑1 (MQ=255) ttctACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAggcggc < 6:358506/48‑1 (MQ=255) ttctACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAggcggc > 5:358506/1‑48 (MQ=255) ctACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGaa > 7:99674/1‑135 (MQ=255) ccTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAAt > 3:236805/1‑134 (MQ=255) gggCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCaa < 8:20423/93‑1 (MQ=255) gggCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCaa > 7:20423/1‑93 (MQ=255) gCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACg > 2:25614/1‑135 (MQ=255) cTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGAGTTTCCGCTTCCAGGTGGCATTCATCCTGTTGAAGGCCAGCCTCACCCAGCTGATTCCACCGCGCGCGGTGATCAAGCAGCACTACAACgg > 4:70112/1‑135 (MQ=255) tCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACGGcc > 7:326150/1‑135 (MQ=255) tCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACGGcc > 1:331798/1‑135 (MQ=255) ttGGCGGCCGCGCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACGGCCATc < 7:58766/135‑1 (MQ=255) tgggcggcCGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACGGCCATc < 7:64723/133‑1 (MQ=255) cggcTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACGGCCATCGCCt < 4:167709/135‑1 (MQ=255) ctgctCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACGGCCATCGCCTcga > 8:370644/1‑135 (MQ=255) | CGATGCCCTGCGCCATGTGGCGCTGAACGTGACCTCGGTGGTCACCACCACCGGCTTCTCCTTGGGTGACTACAGCCTGTGGGGTAACTTCTCGCTGATGCTGTTCTTCTACCTGGGCTTCGTTGGCGGCTGCTCCGGCTCGACCGCAGGCGGCATCAAGATTTTCCGCTTCCAGGTGGCATACATCCTGTTGAAGGCCAGCCTCAACCAGCTGATTCACCCGCGCGCGGTGATCAAGCAGAAATACAACGGCCATCGCCTCGA > ACB122/5115225‑5115488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |