| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,301,325 | A→C | 49.1% | intergenic (‑106/+3467) | recC ← / ← PP_RS08750 | RecBCD enzyme subunit RecC/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,301,325 | 0 | A | C | 49.1% | 1.9 / 3.4 | 20 | intergenic (‑106/+3467) | recC/PP_RS08750 | RecBCD enzyme subunit RecC/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base A (8/2); new base C (8/2); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGC‑GGCCGTGCCC‑GCTCCCG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGG > ACB122/5301198‑5301455 | ggAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGC‑GGCCCGGGCC‑CCGCCCG‑Ccgcggata > 7:212152/1‑131 (MQ=255)ggAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCCGGGGA‑CGCCGGGCCC‑CCCCCCG‑CCGCGaata > 1:208820/1‑132 (MQ=255) tGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGC‑GGCCGTGCCC‑GCTCCCG‑CAGCGATTCGC‑G‑Ca > 8:304522/1‑135 (MQ=255) cGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGCGGGCCGGGCCC‑‑CTCCCC‑CCGCGCTTGGC‑GCCGGGGGGGGTTTTTTGcc > 8:260091/1‑135 (MQ=255) aaGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGC‑GGCCCGGCCC‑GCTCCCC‑CCGCCCGTCGC‑G‑CAGGTCGAGGTTTTTGGCcac > 7:240771/1‑134 (MQ=255) aTGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGC‑CGCCGGGGCC‑CCGCCCC‑CCGCGCTTGGCGG‑CGGGGCCGTGTTTTTGCCC‑CAAAAACAAAACCCCCAccag > 4:311300/1‑133 (MQ=255) gagGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGC‑CGGCGGGGCC‑CCGCCCC‑CCGCGGATAGCGG‑CGGGCGGGGTTTTTTGCCC‑CCAACACAAAACACCCACCAGGGGgcgcg > 7:20955/1‑134 (MQ=255) gagGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGCGC‑GGGCGGGGCC‑GCGCCCG‑CCGCGCTTGCC‑G‑CCCGGGGCGGTTTTTGGCCACAAAAACAAAAACCCAACCagcgtgc > 2:258673/1‑132 (MQ=255) gTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGGC‑GGCCGGGGCC‑CCGCCCC‑CCCCGCTTGGC‑G‑CAGGGGGAGGTTTTTTGCCACAAAGACAAAAACCCCACCAGCGGGCGCCCATAGGGGGTTTGGGCAAt > 2:237849/1‑135 (MQ=255) aCATGAAACGGCCGAATGCCATCAGGCTGCGGGCGC‑GGCCGTGCCC‑GCTCCCG‑CAGCGCTTCGC‑G‑CAGGTCGACTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCCCAGAAAGGGGTTTTGGGAAAtt > 8:317486/1‑133 (MQ=25) aTGAAACGGCCGAATGCCATCAGGCTGCGGGGGC‑GGGCCTTCCC‑CCGCCCC‑CCGCGCATACC‑G‑CAGGGCGAGGTTTTTTGCCACAAAAACAAAAACCCAAACAACGGGCGCCCACAGGGGTTTTTGGaaaataa > 6:199886/1‑130 (MQ=255) gggtggc‑gggcggGCCC‑GCGCCCGACCGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATgg < 8:330044/130‑1 (MQ=14) gccgggCCGGGCCCGGCCCCCG‑CCGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGa < 1:207496/131‑1 (MQ=14) ggCCGTGCCC‑GCTCCCG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAAccc < 8:129861/135‑1 (MQ=255) gTGCCC‑GCTCCCG‑CAGCGATTAGC‑G‑CAGGTGGAGTTTTTTTCCCACAAAGACAAAAACCCAACCAGCGTGCGGAGGTTGGGGGTTTGCGAAATGAATGTTGTAGATGACCTACTCTCACAAGGGGAAAccccccc > 2:295903/1‑132 (MQ=255) gccc‑gcccCCG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATgggg > 6:375220/1‑123 (MQ=255) gCCC‑GCTCCCG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAAAGAATCTTGACGATGCCCTACACGCACATGGGGCAACCCCCCACt > 7:248739/1‑135 (MQ=17) gCCC‑CCCCCCG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATgggg < 5:375220/123‑1 (MQ=255) gCTCCCG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCa > 4:150794/1‑135 (MQ=255) cG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTCCTCTCACATGGGGAACCCCCACACTACCATCgg > 7:254539/1‑134 (MQ=255) cG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCgg > 8:305414/1‑134 (MQ=255) | GGAGATGGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGAGC‑GGCCGTGCCC‑GCTCCCG‑CAGCGATTCGC‑G‑CAGGTCGAGTTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGG > ACB122/5301198‑5301455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |