Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,533,095 G→A 100% R132C (CGC→TGC)  CJ019_05210 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,533,0950GA100.0% 334.9 / NA 91R132C (CGC→TGC) CJ019_05210hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/55);  total (36/55)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCATCGT  >  ACB122/5532966‑5533226
                                                                                                                                  |                                                                                                                                   
cGTGGGACCGGCGCGGGCCCCCCACTACAACCGCACGCGCGCCTTTGCCGAACA‑CAGGCAGGCCGGCGATGGCACGTATCTGCACGGAGTCACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCa                                                                                                                                <  8:319488/135‑1 (MQ=255)
 gTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAg                                                                                                                               <  4:303037/135‑1 (MQ=255)
    ggACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATgcagcgcagc                                                                                                                              >  2:359023/1‑133 (MQ=255)
    ggACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATgcagcgcagc                                                                                                                              >  1:41973/1‑133 (MQ=255)
    ggACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGa                                                                                                                            <  5:368483/135‑1 (MQ=255)
        aGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGcc                                                                                                                        >  2:71359/1‑135 (MQ=255)
           cgcTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCg                                                                                                                     <  7:150000/135‑1 (MQ=255)
             cTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGAt                                                                                                                   >  3:239874/1‑135 (MQ=255)
                  ccACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATgccgc                                                                                                              >  1:57929/1‑135 (MQ=255)
                       tCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCTTCGATCAGGATCGAGTGACGAACCTTGTCCTGACAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGACGCCGAGc                                                                                                         >  8:260081/1‑135 (MQ=255)
                        cGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTAACGAACCTTGTCCTTCCACTAGACGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCCAGCa                                                                                                        >  6:241333/1‑135 (MQ=255)
                          accaccGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGAGCAGGATCGAGTGACGAACCTTGTTCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  2:199665/134‑1 (MQ=255)
                          accaccGCACGCTCGGAGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGCTCGAGTGACGAACCTTGGCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  2:209478/134‑1 (MQ=255)
                              ccGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCg                                                                                                  <  1:363187/135‑1 (MQ=255)
                                gCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGc                                                                                                <  4:239874/135‑1 (MQ=255)
                                gCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGc                                                                                                <  2:310424/135‑1 (MQ=255)
                                gCACGCTCGGCGTTGCCGAACAGCAGG‑ATGGCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGc                                                                                                <  2:253379/135‑1 (MQ=255)
                                 cACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGca                                                                                               <  1:30008/135‑1 (MQ=255)
                                  aCGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGCCGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGCGCACGATCGGcac                                                                                              >  2:161116/1‑135 (MQ=255)
                                  aCGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGcac                                                                                              >  6:172626/1‑135 (MQ=255)
                                   cGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGcaca                                                                                             <  8:182809/135‑1 (MQ=255)
                                      tCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGc                                                                                          <  2:269560/135‑1 (MQ=255)
                                      tCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGAGCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGc                                                                                          <  2:45416/135‑1 (MQ=255)
                                          cGTTGCCGAACAGCAGG‑ATGTCGGCTCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      <  8:103038/135‑1 (MQ=255)
                                          cGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      <  6:245523/135‑1 (MQ=255)
                                          cGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      <  1:212395/135‑1 (MQ=255)
                                          cGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGAGCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      <  2:226573/135‑1 (MQ=255)
                                          cGTTGCCGAACAGCAGG‑ATGGCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      <  7:381509/135‑1 (MQ=255)
                                            ttGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCg                                                                                                  >  3:238900/1‑121 (MQ=255)
                                            ttGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCg                                                                                                  <  4:238900/121‑1 (MQ=255)
                                                  aaCAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGcctcc                                                                              <  7:295399/135‑1 (MQ=255)
                                                       cagG‑ATGTCGGCGCTGTCATCGATCATGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGAAGCGAAGCTAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCa                                                                         >  8:250707/1‑135 (MQ=255)
                                                        agG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                        <  8:248105/135‑1 (MQ=255)
                                                        agG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                        <  8:352957/135‑1 (MQ=255)
                                                        agG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                        <  4:143769/135‑1 (MQ=255)
                                                        agG‑ATGTCGGCGATGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGGAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCCTGTAGGCCTCCTTGCAg                                                                        <  1:341219/135‑1 (MQ=255)
                                                         gg‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  8:156776/135‑1 (MQ=255)
                                                         gg‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  1:54648/135‑1 (MQ=255)
                                                         gg‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  1:52568/135‑1 (MQ=255)
                                                         gg‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  3:4869/135‑1 (MQ=255)
                                                          g‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgcg                                                                      >  6:339640/1‑135 (MQ=255)
                                                          g‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgcg                                                                      >  8:355232/1‑135 (MQ=255)
                                                                   cgcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCtat                                                              >  6:360411/1‑135 (MQ=255)
                                                                    gcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCtata                                                             <  1:3278/135‑1 (MQ=255)
                                                                    gcTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCtata                                                             <  8:135679/135‑1 (MQ=255)
                                                                     cTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGc                                                                                                <  8:319573/99‑1 (MQ=255)
                                                                     cTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGc                                                                                                >  7:319573/1‑99 (MQ=255)
                                                                              aTCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTg                                                   <  1:63496/135‑1 (MQ=255)
                                                                              aTCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTg                                                   <  5:172626/135‑1 (MQ=255)
                                                                                   gATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACg                                              <  8:19502/135‑1 (MQ=255)
                                                                                     tCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGt                                            >  8:175537/1‑135 (MQ=255)
                                                                                     tCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGt                                            >  8:228477/1‑135 (MQ=255)
                                                                                       gAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTcc                                          <  7:374915/135‑1 (MQ=255)
                                                                                        aGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  4:305254/73‑1 (MQ=255)
                                                                                        aGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       >  3:305254/1‑73 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       >  8:131971/1‑70 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCAc                                                                                                       <  7:131971/70‑1 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       >  4:214925/1‑102 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAgc                                                                       <  3:214925/102‑1 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                        <  1:220112/101‑1 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAg                                                                        >  2:220112/1‑101 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGAc                                      >  3:370941/1‑135 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGAc                                      >  5:27218/1‑135 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGAc                                      >  7:111125/1‑135 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGATTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGAc                                      >  3:54592/1‑135 (MQ=255)
                                                                                           gACGAACCTTGTCCTGCCAGAAGTCGTAGAGGGCGATGCAGCGCAGCGAAGCCTCGATGACGCCGAGCACGATCGCCACATGCTTGTAGGCCTCCATGCAGCGCTGGCTATACACCAGGCTGCCACGGACCggcc                                      >  3:18358/1‑133 (MQ=255)
                                                                                                aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTgg                                                                  <  4:281858/102‑1 (MQ=255)
                                                                                                aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTgg                                                                  >  3:281858/1‑102 (MQ=255)
                                                                                                aCCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTg                                 >  3:344464/1‑135 (MQ=255)
                                                                                                   ttGTCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCg                              <  3:118663/135‑1 (MQ=255)
                                                                                                      tCCTGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggc                            <  1:246417/134‑1 (MQ=255)
                                                                                                      tCCTGCCAGTAGTCGTAGGGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggc                            <  1:87360/134‑1 (MQ=255)
                                                                                                         tGCCAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccagg                        >  6:3901/1‑135 (MQ=255)
                                                                                                           ccAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcc                      <  5:339640/135‑1 (MQ=255)
                                                                                                            cAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                     <  2:222189/135‑1 (MQ=255)
                                                                                                            cAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                     <  3:36044/135‑1 (MQ=255)
                                                                                                            cAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                     <  3:31278/135‑1 (MQ=255)
                                                                                                            cAGTAGTCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcc                      <  8:287273/134‑1 (MQ=255)
                                                                                                            cAGTAGTCGTAGTGGCCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                     <  6:218728/135‑1 (MQ=255)
                                                                                                            cAGTAGTCGTAGGGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                     <  2:133314/135‑1 (MQ=255)
                                                                                                             agtagtCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                     >  6:149262/1‑134 (MQ=255)
                                                                                                              gtagtCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                     >  4:112336/1‑133 (MQ=255)
                                                                                                              gtagtCGTAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggccaggcca                     >  8:213202/1‑133 (MQ=255)
                                                                                                                     tAGTGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGgt             >  7:8398/1‑134 (MQ=255)
                                                                                                                        tGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggc                            <  3:68869/116‑1 (MQ=255)
                                                                                                                        tGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCggc                            >  4:68869/1‑116 (MQ=255)
                                                                                                                        tGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTa          <  2:57929/134‑1 (MQ=255)
                                                                                                                        tGGGCGATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCGGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTa          <  7:175537/134‑1 (MQ=255)
                                                                                                                             gATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCAtc    >  8:173696/1‑135 (MQ=255)
                                                                                                                             gATGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCAtc    >  7:109366/1‑135 (MQ=255)
                                                                                                                               tGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      <  4:52240/51‑1 (MQ=255)
                                                                                                                               tGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGt                                                                                      >  3:52240/1‑51 (MQ=255)
                                                                                                                               tGCAGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCAtcgt  >  3:160547/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                   
CGTTGGACAGGCGCTGGGCCACCTCGACCACCGCACGCTCGGCGTTGCCGAACAGCAGG‑ATGTCGGCGCTGGCATCGATCAGGATCGAGTGACGAACCTTGTCCTGCCAGTAGTCGTAGTGGGCGATGCGGCGCAGCGAAGCCTCGATGCCGCCGAGCACGATCGGCACATGCTTGTAGGCCTCCTTGCAGCGCTGGCTATACACCAGGCTGGCACGGTCCGGACGGCTGCCGGCCAGGCCACCAGGGGTGTAGGCATCGT  >  ACB122/5532966‑5533226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑