| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,688,637 | A→C | 64.1% | S236R (AGC→CGC) | pyrC' → | Dihydroorotase‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,688,637 | 0 | A | C | 64.1% | ‑3.1 / 9.6 | 34 | S236R (AGC→CGC) | pyrC' | Dihydroorotase‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (3/8); new base C (7/15); total (10/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01 | |||||||||||
TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCA > ACB122/5688503‑5688767 | tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCCACCTGC‑TGCTGGTGGAACAGAGCGGCGGGCGGGGGCCCGTCACCCACATCACCa > 8:181401/1‑135 (MQ=255) cACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCCGATCACCaccccc > 4:323835/1‑130 (MQ=255) aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCAGCCAGCTCACCACCGCCCgggg > 7:221132/1‑135 (MQ=255) ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGGGCCCTTCAGCCCGAACACCCGCCCCCCTGGcgcgcg > 3:179809/1‑130 (MQ=255) ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGGGCGCAGCATCCCGCTCACCCCCGCCCGGGGCGCGCGGcggc > 2:182527/1‑132 (MQ=255) aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGGGGCCTTCAGCCAGATACCCCCCGCCCCGGGCGCGCGGCGGATc > 6:199004/1‑135 (MQ=255) aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc < 8:74464/135‑1 (MQ=255) ttCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCCGCCCGATAACCCGCGCCCGTGGCGCGCGGCGGATCgcc > 3:94042/1‑135 (MQ=255) tCGGCCTGCCGGGCATCCGGGAGAGCGCCTAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGGGTAACAGAGCGGCGTGCGGGCGCACTTCACCCATATCACCCGCGCCCGGGGGGCGCGGCTGATCgcccagg < 7:314286/135‑1 (MQ=255) ccTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCCGGCGCACATCAGCCACCTAACCCGCCCCCCGGGCCCGCGGCTGCTCgcccacgcccc > 6:267640/1‑134 (MQ=255) cgccgcgcgcGGAAACCGTGGCCCTGGCGCGCAACCGGC‑GGCGGGTGGAACAGAGCGGGGTGCGGGCGCACTTCACCCCGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGc < 5:247445/129‑1 (MQ=255) ccgagCGCCTAACCGGGGGCCGTGGCGCCCACCGTGC‑GGCTGGGGTAACAGCGGGGCGTGGGTGCGAACTTCCGCCACATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCg < 2:66435/133‑1 (MQ=255) gcccgcCGAAACCGTGCCCGGGGCGCGCACCCTGC‑GGCTGGTGCAACTGCGGGGGGGGGGGGCGCAGTTCACCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt < 2:285614/132‑1 (MQ=255) cgcCGAAACCGGGGCCCTGGCGCGCAACCTGC‑TGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 4:157324/135‑1 (MQ=255) cgcCCAACCCGGGCCCCGGGCGCGCACCCTGG‑TGGTGGGGTAACTGCGGGGCGGGGGGGCGCACTTCAGCCAGATCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc < 3:313229/135‑1 (MQ=255) gcCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTGCGCCCGATCACCCCCGCCCGAGGCGCGCGGCGGCTAGCCCCCGCCCCGGCCGTGGGGCTGCCCGGAAcc > 6:267455/1‑135 (MQ=255) aaccggTGCCCCGGGCGCCCAACCGGC‑TGCGGGGGCAAGAGCGGGGGGTGCTGGCGCATTTCCCCCACTTCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 5:271621/130‑1 (MQ=255) cccggggccggggCGCGCACCCGGC‑GGGTGGGGGACCAGCGCGGGGGGCGGGCGCACTTCACCCAGACCACCAGCGCCCGTGGCCCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg < 5:48662/132‑1 (MQ=255) ggggCCGTGGCGCGAAAGCTGC‑TGCTGGGGTAACTTAGCGGGGGGCGGGCGCAGTTCAGCCAGATCCCCCGCGCCTGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 4:273668/133‑1 (MQ=255) ggggCCCTGGCGCGCAACGTGG‑TGCGGGGGCAACAGCGCGGCGGGCGGGCCCAGTTCCCCCAGAACCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 3:136654/133‑1 (MQ=255) ggggCCCTGGCGCGC‑ACCGGCGGGCGGGGGCAACCGGGCGGGGTGGGGGCGCACTCCCGCCCCACCCCCCGCGCGCGTGGCGCGCGGCGGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 1:157683/133‑1 (MQ=255) gTGGCCCTGCCGCGCAACCTGC‑TGCTGGGGGAACACAGCGGCGTGCGTGCGCACTTCCGCCAGATCCCCAGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 7:103754/135‑1 (MQ=255) gTGGCAGTGGGGGGCATCCTGG‑TGCTGTGTGAAGTGTGCGGCGTGCGTGCGCACTGAAGCCTTTTCACCTGCGCGCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa < 8:188382/135‑1 (MQ=255) gcAACCTGC‑TGGTGGTCGAACAGTGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGc < 2:264135/135‑1 (MQ=255) gAACAGAGCGGCGTGCGTGCGCCCTTCAGCCCGATCACCCCCGCCCCGGGCGCGCGGCTGCTCGCCCCCGCCCCGGCCTTGGGCCTGCCCGGAACCGCCGCTGTAGCGCGGTACCAGCTGCTCCTCACGGCCGAg > 6:109227/1‑135 (MQ=255) agCGGCGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg < 4:193643/135‑1 (MQ=255) cggcggGCGTGCGCACTCCAGCCAGACCCCCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg < 7:68970/135‑1 (MQ=255) ggcgtgcgCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 4:94042/134‑1 (MQ=255) ggcgtgcgCACTCCAGCCAGAGCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 4:130142/134‑1 (MQ=255) ggcgggcgCCCTCCCGCCACACCCCCCGCGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 2:154422/134‑1 (MQ=255) ggcgggccCACTTCCCCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt < 5:375260/134‑1 (MQ=255) ggtgcgATCTTCAGCCACTGCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCATGGACGAGTCGCTGCGCGAGTtc < 7:342314/134‑1 (MQ=255) gcgcACTTCCCCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc < 3:323835/135‑1 (MQ=255) gcAGTTCACCCAGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCTAGTTCTCCAg < 3:287550/135‑1 (MQ=255) cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGGAACACCTGCa > 7:325504/1‑135 (MQ=255) cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCACGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTGTCCAGCCAGTACCACGTGCa > 1:371894/1‑135 (MQ=255) | TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCA > ACB122/5688503‑5688767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |