| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 6,001,307 | A→C | 34.7% | S313R (AGC→CGC) | mdtK → | Multidrug resistance protein MdtK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 6,001,307 | 0 | A | C | 34.7% | 15.7 / 5.0 | 38 | S313R (AGC→CGC) | mdtK | Multidrug resistance protein MdtK |
| Reads supporting (aligned to +/- strand): ref base A (13/10); new base C (11/3); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
CACCGAACTGGCGGCGCACCAGATTGCCCTGCAGATCGTGTCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGTTCATCGACCGTAATGACCCGGCCTTTGCCGCGATCTTCCAG > ACB122/6001174‑6001441 | caCCGAACTGGCGGCGCACCAGATTGCCCTGCAGATCGTGTCCACGGCATTCATGGTGCCGACTCGCTTGTCCTATGCGGTGACCATGCCCGTCGGCCGGTACTACGGCGCTGGCAACCAGCTGGAGGCCCGCAg > 7:279330/1‑135 (MQ=255) cGAACTGGCGGCGCACCAGATTGCCCTGCAGATCGTGTCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAgcgc > 7:189522/1‑135 (MQ=255) tGGGGGGGCACCAGTTGGCCGTCCATTACGTGTCCAGGGCATGCATGGTGCCGAAGGGGTTGACCTAGGCGGTCACAAGCCGCGCGGGCCTGACCTCCGGCGGGGGCAACAGGATGGGGGCGCGCAGCGCcgggc < 8:100619/135‑1 (MQ=255) tGGCGGCGCACCAGATTGCCCTGCAGATCGTGTCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCACCCTGCTGGCGGCGCGCCCCGCcgggc > 8:183821/1‑135 (MQ=255) tGGCGGCGCACCAGATTGCCCTGCAGATCGTGTCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGGCGGCAGCGCcgggc > 5:373215/1‑135 (MQ=255) ccTGCAGATCGTGTCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGGGGGCCT‑CGGGTTTgg > 8:93259/1‑135 (MQ=255) aTCGTGTCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGGGCGCCGCGCCCGGG‑GGGGGGGGCG‑CGGGGTTggggggcgg > 1:140445/1‑130 (MQ=255) cggttgccAGGGCATTATTGGGGCCGACTGGTTGGGCCTTTGGGGTGCCCATGCGCTGCGGCGTGTACTACGGCGCGGCCACCGGGGGGGGGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCAt < 2:234612/127‑1 (MQ=255) gtCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGGGCCCCGCCCCCGGCGGGGGGGGCGA‑CGGGTTTGGGGCCagctgaa > 2:175285/1‑129 (MQ=255) gtCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCCGCGCGCCGCGCCCGGC‑GGGTGGGCAT‑CGGGTTTTGGGCCATGATCAt > 6:74135/1‑135 (MQ=255) cACGGCATTCATGGGGCCGACTGGCTTGTCCTAGGCGGGGCCCATGCGCGTCGGCCGGTACTACGGCGCTGGCAACCGGCGGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGtt < 7:46210/135‑1 (MQ=255) aTTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCCGCGCCCGGC‑GGGTGGGCCT‑CGGGGTTTGGGGCAAGATAATtttttgctt > 1:90876/1‑130 (MQ=255) ttGGGGCCGACTGGCTTGTCCTATCCGTTGACAATGCGCGTCGGCCTGAACAACGGCGCTGGCAACCTGCGGGCGGCGGGCTGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGc < 2:220846/133‑1 (MQ=255) agggggCCGACGGGGTTGTCCTATGCGGTGACCATGCGCGGCGGCCGGTACTCCGGCGCTGGCAACCTGCGGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGcc < 2:106104/130‑1 (MQ=255) tGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGGCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGcc < 8:189522/134‑1 (MQ=255) ggCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGGCGCGCCCGGC‑GGGGGGGGATCCGGGTTGGGGGCCA‑GATACTGTTTTCCTTCGCCCCGCTGTTGctg > 2:167221/1‑135 (MQ=255) gCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGGACTACGGCGCTGGCAACCTGCTGGCGGCGCGCCCCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATTCTCATGTTTGCCGTCGCCACGCTGGtgctgc > 7:281036/1‑135 (MQ=255) gTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCctgctgct > 2:19631/1‑135 (MQ=255) gCGGGGACCATGCGCGGCGGCCTGTCCTCCGGCGCGGGCAACCTGCTGGCGGCGCGCCGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCg < 2:209272/133‑1 (MQ=255) ggTGACCATGCGCGTCGGCCTGTACTACTGCGCTGGCAACCTGCTGGCCGCCCCCAACGCAGGGC‑CCGTTTGCAA‑GGGGGTTATGGCCAAGAAAAATTTATCCATAGCCACGCAGGTCATGCTTCTGCCCGAAGc > 8:63643/1‑135 (MQ=255) ggTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCCGCGCCGGGC‑GGGTTGGGCT‑CGGGTGTGGGGCCCTGATCATGTTTGCCTTCGGCACGCTGTTGCTGCTGCTGCCCGaacc > 1:80983/1‑133 (MQ=255) ggTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCCACCCCGGGG‑GGGTGGGCAT‑CGGGGTTTGGGGCAAGCTCAATGTTGCCGTCGGCACCCGTTTCCTTCTGCTGcccccagc > 8:313639/1‑130 (MQ=255) ggTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGGCT‑CGGGTTTGGGGGCCTGATCATGTTTTCCGTAGCCCCGCGGGTCCTGCTGCTGCCCGGAGc > 5:272465/1‑135 (MQ=255) ggTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGGGGGCAT‑CGGGTTTGGGGCCCTGTTGATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGCAGc > 2:38137/1‑135 (MQ=255) tGACCATGCGCGTGGGCGGGTACAACGGCGGGGGAAACCTGCGGGCGGCGCGCAGCGCCGGGC‑GGGGGGGCAT‑CGGGTGTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCAc < 8:279330/135‑1 (MQ=255) ccATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAGCGCCCGGC‑GGGTGGGCAT‑CGGGGTTGGGGCCATGATCATGTTTGCCATAGCCCCGCAGTTGCTGGTGCTGGCCGAAGCACTgg > 4:29526/1‑135 (MQ=255) tGCGCGTCGGCCTGAACAACGGCGCTGCCAACCTGCTGGCGGGGCGCCGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTTGGGCCATGATCTTGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAg < 7:271004/135‑1 (MQ=255) cGGCCTGTACTACGGCGCGGGCAACGTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGtt < 7:9674/135‑1 (MQ=255) gCCTGTACTACGGCGATGGCAACCTGCTGGCGGCGCGCCGCGCCCGGC‑GGGTGGGCCT‑CGGGGGTGGGGCCATGATAATGTTTGACATCGCCACGCTGTTCCTGCTGCTGCCCCAAGCCCTGGTAGGCCTggtga > 6:272637/1‑131 (MQ=255) ccTGTCCTCCGGCGCTGGCACCCGGCGGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGGTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGTTCAt < 8:46303/135‑1 (MQ=255) cTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCCGCGCCCGGC‑GGGTGGGCAT‑CGGGTGTGGGGGCATGATCATGTTTGCCGTAGCCCCGCTGTTCCGGCTGCTGCCCGTAGCCCTGGTAGGCCGGTTCAta > 4:354965/1‑134 (MQ=255) cctccGGCGCGGGCACCCTGCTGGCGGGGGGCCGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGTTCATCGAcc < 3:341562/131‑1 (MQ=255) gCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGGAT‑CGGGTTTGGGGCCATGATCATGTTTGCCGTCGCCACGCTGTTCCTGCGGCTGCCCGCAGCACTGGGAGGCCGGTTCATCGCCCGGAATGAccctg > 8:264449/1‑133 (MQ=255) gCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTTGGGCCATGATCATGTTTTCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGTTGATCGACCGTAATTAACCgg > 8:193227/1‑135 (MQ=255) gCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAA‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTATGCCTGTTCATCGCCCGTAATGACCCgg > 8:376482/1‑135 (MQ=255) gCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGGGGGCCT‑CCGGTTTGGGGGCCTGATCATGTTTGGCTTCGCCACCCGGATGCTGCTGCTGCCCGCAGGACGGGTAGGCCGGGTCATCGCCCGGAATGAccccg > 6:369070/1‑133 (MQ=255) ctgGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGTTCATCGACCGTAATGACCCGGCCTTTGcc < 8:372161/135‑1 (MQ=255) ctgGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGTTCATCGACCGTAATGACCCGGCCTTTGcc < 7:247193/135‑1 (MQ=255) aGCGCCGGGC‑GGGTGGGCAT‑CGGGTTGGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGTTCATCGACCGTAATGACCCGGCCTTTGCCGCGATCTTCCAg > 2:57826/1‑135 (MQ=255) | CACCGAACTGGCGGCGCACCAGATTGCCCTGCAGATCGTGTCCACGGCATTCATGGTGCCGACTGGCTTGTCCTATGCGGTGACCATGCGCGTCGGCCTGTACTACGGCGCTGGCAACCTGCTGGCGGCGCGCAGCGCCGGGC‑GGGTGGGCAT‑CGGGTTTGGGGCCATGATCATGTTTGCCTTCGCCACGCTGTTCCTGCTGCTGCCCGAAGCACTGGTAGGCCTGTTCATCGACCGTAATGACCCGGCCTTTGCCGCGATCTTCCAG > ACB122/6001174‑6001441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |