Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,020,344 T→G 29.6% S57R (AGC→CGC)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,020,3440TG29.6% 27.6 / 2.3 43S57R (AGC→CGC) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base T (11/16);  new base G (5/9);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGC  >  ACB122/6020216‑6020474
                                                                                                                                |                                                                                                                                   
ccGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGGCCGGCTGGCGcc                                                                                                                               >  6:150812/1‑135 (MQ=255)
ccGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGGTGGCCCCGCTGGCGcc                                                                                                                               >  1:33043/1‑135 (MQ=255)
ccGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGcc                                                                                                                               <  6:335829/135‑1 (MQ=255)
          agcCATACGGGGTAACTCGGCAGCCGCGCGAGCAGCCCAATCGACCGCGCCCCGCCCCACCGCCACCGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCTGGCTGGCGCCGGCCA‑GTACg                                                                                                                    <  2:338770/135‑1 (MQ=255)
                         cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCACGCAGGCGCCGGCCA‑CTAACCCCAACCCCGGCAtt                                                                                                     >  4:124213/1‑135 (MQ=255)
                           aGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCACGCTGGCGCCCGCCC‑GTACGCCCACCGCCGGccatgc                                                                                                   >  2:235289/1‑130 (MQ=255)
                                 cagcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCA‑GTAAGCCCCACGCCGGCATTgccgccc                                                                                              >  8:265831/1‑133 (MQ=255)
                                  agcGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCACGCGGGCGCCCGCCC‑CGACGCCCAACACCCGCATTGGCGCCCTc                                                                                            >  3:52407/1‑135 (MQ=255)
                                       gAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCGGGCGGCGGCCA‑GTACGCCCCCCCCCGGCCTTGCCGCCCTCCCGcc                                                                                       >  7:103718/1‑135 (MQ=255)
                                            ggCAAATCGACCGCGCCCCGCCCCACCGCCCCCGGCGCAGCGCCGACGGTACGCCAACCCACCCTCCCCGGCGGCGGGCCAGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATgg                                                                                  <  5:154278/135‑1 (MQ=255)
                                                    gACCGCCCCCCGCCCCACGGGCACCGGCGCATCGCCGAAGTTACCCCAACCCACCCTCCCGGGCGGGGGGCAGGGGGGGGGCCGGCTT‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTg                                                                          <  2:82832/135‑1 (MQ=255)
                                                     aCAGCGCCCCGCCCCACAGCCACAGGCGCAGCGCCGAAGGTACCCCAACCCACCCTGCCAGCGGGCTGGCCCGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGc                                                                         <  7:4613/135‑1 (MQ=255)
                                                          gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGTGCCGGCCC‑GTACGCCCCACGCCGGCCTTGCCGCCCTCACGCCGATGGCGAAGCTGCCGTAg                                                                    >  6:326185/1‑135 (MQ=255)
                                                          gccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCGGGCGCCGGCCC‑GTACGCCCCACGCCCGCCGTGCCGCCCTCCCCCCGCAGGCCAAGCTTCCGTAg                                                                    >  6:360349/1‑135 (MQ=255)
                                                              cgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCCACCCAACCTGCCAGGCGGCTGGCCCGGCAGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGcccc                                                                >  4:264952/1‑135 (MQ=255)
                                                                   cccaggcactggcGCAGCGCCCAAGGAACGCCAACCCAACCTGCCCGGCGGGTGGCCTGGCTGGCGCGGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGcc                                                           <  1:74419/133‑1 (MQ=255)
                                                                        gcacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGGCCGGCGGGCGCCCGCCC‑GGTCGCCCCACGCCCGCAAGGCCCCCAGCCCGCCGCTGGCGAAGCAGACGGCGCCCCCCGCCGCCac                                                      >  8:170687/1‑134 (MQ=255)
                                                                           caggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCGCCGGCCAGGCAGGCCCCCCCCCGCCAGT‑CCCCCAACCCCCCCCTGGCGCCGCTGGCGTAGCCCCCGGCCTccccgcc                                                   >  2:338079/1‑130 (MQ=255)
                                                                            aggcGCATCGCCTAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgcgc                                                  <  2:73769/135‑1 (MQ=255)
                                                                              gcgcATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGCGCCGGCCA‑GGACGCCCCACCCCCGCATTGCCGCCCACACCCCGAAGGCGAAGCTGCCGTAGCCCCCCGCCTCCACGCGCAg                                                >  6:379985/1‑135 (MQ=255)
                                                                                 aaTCGCCGAAGGTACGCCAACCCAACCTGCCCGGCGGGTGGCCAGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGa                                             <  7:26685/134‑1 (MQ=255)
                                                                                     gCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCGCCGGCCC‑GTACGCCCCCCGCCGGCATTGCCGCCCTCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCGCCCGGCGCAgggccccc                                         >  3:344259/1‑130 (MQ=255)
                                                                                     gCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCAGGCCAGGCTGGCGCCGGGCA‑GTACGCCCAACGCCGGCATTTCCGCCATCACCCCGCTGGCGCAGCTGCCGTAGCCCCAGGCCTCCAGGCGCACGGCCAcc                                         >  2:78032/1‑135 (MQ=255)
                                                                                       cGATGGTACGCCACCCCACCCTGCCCGGCGGCTGGCCTGGCTGGCGCCGGCCA‑GAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAg                                       <  8:178358/135‑1 (MQ=255)
                                                                                       cGACGGTCCCCCACCCCACCCGGCCCGGCGGCGGGCCCGGGGGGCGCCGCCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAg                                       <  3:287724/135‑1 (MQ=255)
                                                                                       cGAAGGTACGCCAACCCACCCGGCCAGGCGGCGGGCCAGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAg                                       <  3:314990/135‑1 (MQ=255)
                                                                                        gAAGGTACGCCAACCCACCGTGCCTGGCGGCTGGCCAGGCTGGCGCCGCCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGc                                      <  7:234436/135‑1 (MQ=255)
                                                                                        gAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGc                                      <  4:52407/135‑1 (MQ=255)
                                                                                             aaCGCCACCCCAACCTGCCAGGCGGCTGGCCAGGGGGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                                 <  2:344490/134‑1 (MQ=255)
                                                                                                        aaccTGCCAGGCGGCTGGCCCCGCTGGCGCCGGCCC‑GTACGCCCCACCCCCGCATTGCCGCCCTCACGCCGATGGCGAAGCTGCCGTAGCCCCCGGCCGCCAGGCGCCGGGCCCCCCGCGGCCTGcgcccgccca                      >  7:215969/1‑133 (MQ=255)
                                                                                                         cccTGCCCGGGGGGGGGCCAGGCTGGCGCCGGCAA‑GTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  5:360349/134‑1 (MQ=255)
                                                                                                         accTGCCCGGCGGCTGGCCAGGCGGGCGCCGGCCA‑GTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  1:50617/135‑1 (MQ=255)
                                                                                                         accTGCCAGGGGGCGGGCCAGGCTGGGGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  2:33043/135‑1 (MQ=255)
                                                                                                         accTGCCAGGCGGCTGGCCAGGCTGGCGCGGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  7:265831/135‑1 (MQ=255)
                                                                                                              cccggcggcTGGCCAGGCGGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCa                <  3:189825/132‑1 (MQ=255)
                                                                                                                aGGCGGGTGGCCTGGCGTGGGCCGGCAA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCTAAGCTTGCGTAGCCCCAGGCCTCAAGGCGCAGGGTCACCAGCGTCATGCTCACGCCATGCTCCAGc              <  8:103078/135‑1 (MQ=255)
                                                                                                                   gggcTGGCCTGGGTGGCGCCGGCCA‑GAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg           <  7:345857/134‑1 (MQ=255)
                                                                                                                   cggcggGCCAGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCCGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg           <  7:24795/135‑1 (MQ=255)
                                                                                                                   cggcTGGCCCGGCTGGCGCCGGCCA‑GGTCGCCCCACGCCCGCATTGCCGCCCTCACGCCGATGGCGAAGCTGCCGTAGCCCCCGGCCGCCCGGCGCAGGGACCCCCGCCGCATGCTCACGCCAAGCCCCCCCCCg           >  6:65868/1‑135 (MQ=255)
                                                                                                                   cggcTGGCCCGGCTGGCGCCCGCCC‑GTACCCCCCACCCCCGCATTTCCCCCAGCACGCCCAAGGCGAAGCTGCCGTAGCCCCCCGCCTCCAGGCGCACGGCCACCAGCCGCCAGCTCACGCCAACCGCCAGcccc           >  4:336976/1‑134 (MQ=255)
                                                                                                                   cggcTGGCCCGGCGGGCGCCGCCCA‑GTACGCCCAACGCCTGCATTGCCGCCAGCAGGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTACAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg           <  3:295659/135‑1 (MQ=255)
                                                                                                                            cggCGGGGGCCGGCCT‑GTCCCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGc  <  5:319290/134‑1 (MQ=255)
                                                                                                                            aGGCGGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGc  <  5:142506/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                                   
CCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCGCCGGCCA‑GTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGC  >  ACB122/6020216‑6020474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑