Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	6,073,990	G→T	5.4%	S72* (TCG→TAG)	CJ019_05690 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	6,073,990	0	G	T	5.4%	243.5 / 4.1	74	S72* (TCG→TAG)	CJ019_05690	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/36);  new base T (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CATGGATGCACGCACCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAGCGCCTTCTGGTCCGACCTGGG  >  ACB122/6073859‑6074117                                                                                                                                    |                                                                                                                                caTGGATGCACGCACCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGt                                                                                                                              <  8:38400/135‑1 (MQ=255)      aTGCACGCACCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGAGGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATc                                                                                                                         <  4:98752/135‑1 (MQ=255)           cgcacCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCgc                                                                                                                    <  7:348269/135‑1 (MQ=255)           cgcacCCGGGTACGGCACCAGGAAGAACAACGTGCTGATGCCCTTGGCGTCACTCTCCAGCGGCACAGGGGAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCgc                                                                                                                    <  2:140677/135‑1 (MQ=255)             cacCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCgcag                                                                                                                  <  3:88574/135‑1 (MQ=255)             cacCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCgcag                                                                                                                  <  5:77378/135‑1 (MQ=255)             cacCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCgcag                                                                                                                  <  7:41870/135‑1 (MQ=255)             cacCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCgcag                                                                                                                  <  2:377746/135‑1 (MQ=255)             cacCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCgcag                                                                                                                  <  1:261877/135‑1 (MQ=255)                        ggCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        <  3:363927/134‑1 (MQ=255)                        ggCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        <  6:371913/134‑1 (MQ=255)                        ggCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        <  5:135037/134‑1 (MQ=255)                        ggCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        <  5:67700/134‑1 (MQ=255)                           aCCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        <  2:228304/131‑1 (MQ=255)                           aCCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        >  1:228304/1‑131 (MQ=255)                           aCCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGctc                                                                                                    >  4:99909/1‑135 (MQ=255)                           aCCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGctc                                                                                                    >  7:303540/1‑135 (MQ=255)                             cAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTg                                                                                                  >  6:8990/1‑135 (MQ=255)                             cAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTg                                                                                                  <  8:189448/135‑1 (MQ=255)                               ggAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        >  8:18838/1‑127 (MQ=255)                               ggAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        <  7:18838/127‑1 (MQ=255)                                 aagaaCAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCt                                                                                                >  5:292099/1‑133 (MQ=255)                                       aacGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        <  5:269459/119‑1 (MQ=255)                                       aacGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCtt                                                                                                        >  6:269459/1‑119 (MQ=255)                                       aacGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAg                                                                                            <  1:110132/131‑1 (MQ=255)                                       aacGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAg                                                                                            >  2:110132/1‑131 (MQ=255)                                       aacGTGCTGTTGCCCATGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCAGTCATGGCCGCAACGGTATTGGTCGAGTACATCACCGCAGGCAGGCCCTTGATCTGCTTGAGGCCa                                                                                        >  8:162873/1‑135 (MQ=255)                                         cGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCAcc                                                                                      <  3:374398/135‑1 (MQ=255)                                         cGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCAcc                                                                                      <  4:129578/135‑1 (MQ=255)                                         cGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCAcc                                                                                      <  2:225983/135‑1 (MQ=255)                                         cGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCAcc                                                                                      <  8:199902/135‑1 (MQ=255)                                            gCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCtt                                                                                   >  6:102168/1‑135 (MQ=255)                                            gCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCtt                                                                                   >  3:267500/1‑135 (MQ=255)                                            gCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCtt                                                                                   >  4:276042/1‑135 (MQ=255)                                                 tGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCg                                                                               >  8:11411/1‑134 (MQ=255)                                                 tGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATCTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCg                                                                               >  1:186350/1‑134 (MQ=255)                                                   cccTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGt                                                                             <  3:190686/134‑1 (MQ=255)                                                   cccTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGt                                                                             >  3:256605/1‑134 (MQ=255)                                                      ttGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAg                                                                         >  7:158511/1‑135 (MQ=255)                                                          cGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGTAACTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGc                                                                     <  4:348191/135‑1 (MQ=255)                                                          cGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTg                                                                      <  4:20611/134‑1 (MQ=255)                                                           gTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGc                                                                     >  3:294480/1‑134 (MQ=255)                                                                 ctcCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGc                                                              >  6:154881/1‑135 (MQ=255)                                                                             aGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGtt                                                  >  4:178269/1‑135 (MQ=255)                                                                               ggTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTtgt                                                >  2:253568/1‑135 (MQ=255)                                                                                       cccAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGAt                                         >  8:106304/1‑134 (MQ=255)                                                                                       cccAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGAt                                         >  4:89257/1‑134 (MQ=255)                                                                                            gCGGATGTCCTTGCCATTCATGGCCTCAACGGTCTTGGTCGAGTACATCAACGCAGGCAGGCCCTTGCTCTGATTGAGGCCACCCTTGCCCGTAAAGTTGCCCATCGCTTCCGCCGAGATCAGGTCGAATTAAg                                    >  4:175354/1‑134 (MQ=255)                                                                                            gCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAg                                    >  2:153405/1‑134 (MQ=255)                                                                                            gCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAg                                    >  5:274066/1‑134 (MQ=255)                                                                                            gCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAg                                    >  8:38103/1‑134 (MQ=255)                                                                                            gCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAg                                    >  4:209166/1‑134 (MQ=255)                                                                                            gCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAg                                    >  2:610/1‑134 (MQ=255)                                                                                            gCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGAGTTGAgg                                   >  5:23319/1‑135 (MQ=255)                                                                                            gCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGGTGTGGTCGATTTCAg                                    >  2:98572/1‑134 (MQ=255)                                                                                            gCGGAAGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGAGATCAgg                                   >  2:374616/1‑135 (MQ=255)                                                                                              ggATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCa                                 >  5:227092/1‑135 (MQ=255)                                                                                                    ccTTGACATTCATTGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGTCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGtt                           <  8:52802/135‑1 (MQ=255)                                                                                                      ttGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCg                         <  5:8990/135‑1 (MQ=255)                                                                                                      ttGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCg                         <  2:186350/135‑1 (MQ=255)                                                                                                      ttGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCg                         <  7:96452/135‑1 (MQ=255)                                                                                                           aTTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAgcgc                    <  8:303540/135‑1 (MQ=255)                                                                                                           aTTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAgcgc                    <  8:79963/135‑1 (MQ=255)                                                                                                           aTTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAgcgc                    <  7:297793/135‑1 (MQ=255)                                                                                                                tGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTTCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAGCGCCTTCt               <  3:221369/135‑1 (MQ=255)                                                                                                                tGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTGGATGTGAGGGATTTTTTGGGGCGccggcg               >  5:221573/1‑131 (MQ=255)                                                                                                                     gCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTAAGGCATTTGTTGGAGCGCCTTGTgggac          >  5:382317/1‑132 (MQ=255)                                                                                                                      cAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAGCGCCTTCTGGTCCg         <  3:99909/135‑1 (MQ=255)                                                                                                                             cTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAGCGCCTTCTGGTCCGACCTggg  <  8:28265/135‑1 (MQ=255)                                                                                                                             cTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAGCGCCTTCTGGTCCGACCTggg  <  3:95480/135‑1 (MQ=255)                                                                                                                                 gTCTAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCAcc                                                                                      <  4:300689/47‑1 (MQ=255)                                                                                                                                 gTCTAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCAcc                                                                                      <  4:295713/47‑1 (MQ=255)                                                                                                                                 gTCTAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCAcc                                                                                      >  3:295713/1‑47 (MQ=255)                                                                                                                                 gTCTAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCAcc                                                                                      >  3:300689/1‑47 (MQ=255)                                                                                                                                    |                                                                                                                                CATGGATGCACGCACCCGGGTACGGCACCAGGAAGAACAACGTGCTGTTGCCCTTGGCGTCACTCTCCAGCGGCACAGGGTAGCCACCCAGGCGGATGTCCTTGCCATTCATGGCCGCAACGGTCTTGGTCGAGTACATCACCGCAGGCAGGCCCTTGCTCTGCTTGAGGCCACCCTTGGCGGTAAAGGTGCCCATGGCTTCCGGCGAGTTGTGGTCGATTTCAGGCATTTGTTCGAGCGCCTTCTGGTCCGACCTGGG  >  ACB122/6073859‑6074117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑