Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	6,101,657	C→T	5.7%	G213R (GGA→AGA)	yeiR ←	Zinc‑binding GTPase YeiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	6,101,657	0	C	T	5.7%	223.6 / 2.5	70	G213R (GGA→AGA)	yeiR	Zinc‑binding GTPase YeiR
Reads supporting (aligned to +/- strand):  ref base C (39/27);  new base T (2/2);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCAGCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATC  >  ACB122/6101529‑6101790                                                                                                                                 |                                                                                                                                      tttCAGCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCTCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGAtt                                                                                                                                 <  4:255535/135‑1 (MQ=255)      gCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCGCCTGCAATTACTCCAACAACCCTCTCCCTGCTGCACATGGCAATTCGGTTTAGTCGGGTCAGCCGAGATTGATGCTGGAGCGTTTCTATGTTTGtgg                                                                                                                             >  6:266695/1‑132 (MQ=255)      gCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCTAAGATTGTCg                                                                                                                             >  6:266700/1‑134 (MQ=255)                     ccTGTTGTCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCa                                                                                                             <  2:371290/134‑1 (MQ=255)                       tgttgtCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCaa                                                                                                            >  6:53368/1‑134 (MQ=255)                        gttgtCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGa                                                                                                          >  4:297340/1‑135 (MQ=255)                          tgaccAGGGTGCCACCGCCAGCCAATACTCCAACCACCATCTCCCTCCTGGACAAGGCAAATCGGTACACTCGGGTCACCCCACAATGCTGCTGGAGCGATTCCAACAATGACGACAATCCTGTTTATCAAGAAt                                                                                                        >  7:245407/4‑135 (MQ=255)                            tCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       <  1:10803/134‑1 (MQ=255)                            tCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       <  6:315844/134‑1 (MQ=255)                            tCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTACCTGCTTGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       <  5:15500/134‑1 (MQ=255)                            tCCAGGGTGCCACCGCCAGCCAATACTCCAACAACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATgg                                                                                                      >  2:158239/1‑135 (MQ=255)                                 ggTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTg                                                                                                  >  8:159661/1‑134 (MQ=255)                                 ggTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTg                                                                                                  >  7:235223/1‑134 (MQ=255)                                  gggCCACCGCCATCCAATACTCAAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGGTGGCTCGGGTCAGCCCACAATGCTGCTTGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTc                                                                                                <  8:225372/133‑1 (MQ=255)                                  gTGCCAACGCCAGCAAATACTCCAACAACCCGCTCCCTGCGGGACAAGGCAAATCGGTTGACTCGGGGCAGCCCACAATGCTGCTGTAGCGATTCCAAGATTGTGGAGAATCCTGTTTTTCAAGAATGGGCTGTc                                                                                                <  6:242308/135‑1 (MQ=255)                                      cACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTcaaca                                                                                            >  3:245059/1‑135 (MQ=255)                                         cGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATc                                                                                         >  6:325187/1‑135 (MQ=255)                                         cGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATc                                                                                         >  6:299525/1‑135 (MQ=255)                                              gccaATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCtgt                                                                                      >  3:11728/1‑133 (MQ=255)                                               ccaATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGga                                                                                   <  5:129416/135‑1 (MQ=255)                                                caATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGgag                                                                                  <  2:343600/135‑1 (MQ=255)                                                      tCCAACCACCCTCTCCCTGCTCGACAAGGCAAAACGCTTGACTCGGGTCAGCCCACAATGCTCCTCGAGCGATTCCAAGATTGTCGACAATCATGTTTTTCAAGAAGATGATGTCAAAAATCTCTGGAGATAcct                                                                            >  2:280987/1‑134 (MQ=255)                                                           ccaccCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCg                                                                         >  7:317178/1‑133 (MQ=255)                                                           ccaccCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCg                                                                         >  6:372715/1‑133 (MQ=255)                                                            caccCTCTCCCTGCTGGAGAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGa                                                                      <  5:5158/135‑1 (MQ=255)                                                            caccCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGa                                                                      >  2:254918/1‑135 (MQ=255)                                                            caccCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGa                                                                      >  7:321147/1‑135 (MQ=255)                                                            caccCTCTCCCTGCTGGACAAGGCAAATCGGGTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGa                                                                      <  4:26587/135‑1 (MQ=255)                                                             accCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAg                                                                     >  4:353342/1‑135 (MQ=255)                                                             accCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAg                                                                     >  1:325267/1‑135 (MQ=255)                                                                 tctcCCTGCTTGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCTAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACTATCTGTGGAGATACCGTCGGGAGGCCg                                                                 <  5:266695/135‑1 (MQ=255)                                                                 tctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCTAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCg                                                                 <  5:266700/135‑1 (MQ=255)                                                                 tctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGcc                                                                  >  6:225477/1‑134 (MQ=255)                                                                  ctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATgg                                                                                                      >  1:10764/1‑97 (MQ=255)                                                                  ctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATgg                                                                                                      <  2:10764/97‑1 (MQ=255)                                                                  ctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCgg                                                                >  5:190978/1‑135 (MQ=255)                                                                  ctcCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATCCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTAGGGAGGCCgg                                                                >  3:228612/1‑135 (MQ=255)                                                                            gACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGAc                                                              >  8:15128/1‑127 (MQ=255)                                                                            gACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGAc                                                              <  7:15128/127‑1 (MQ=255)                                                                            gACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAg                                                      >  6:262887/1‑135 (MQ=255)                                                                             aCAAGGCAAATCGGGTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCa                                                       <  5:123880/133‑1 (MQ=255)                                                                                    aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACTATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  5:86059/135‑1 (MQ=255)                                                                                    aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGCCAAAGGCAGCTGCCCtt                                              >  2:282231/1‑135 (MQ=255)                                                                                    aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  3:138276/135‑1 (MQ=255)                                                                                    aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              >  6:53417/1‑135 (MQ=255)                                                                                    aaaTCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  8:321147/135‑1 (MQ=255)                                                                                    aaaTCGGATGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  6:34028/135‑1 (MQ=255)                                                                                    aaaGCGGTTGACTCGGGGCAGCCCACAAGGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCtt                                              <  5:220626/135‑1 (MQ=255)                                                                                         ggTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACg                                         >  7:91727/1‑135 (MQ=255)                                                                                         ggTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACg                                         >  8:54794/1‑135 (MQ=255)                                                                                              aCTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       >  5:360144/1‑68 (MQ=255)                                                                                              aCTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATg                                                                                                       <  6:360144/68‑1 (MQ=255)                                                                                              aCTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTcc                                     <  6:142139/134‑1 (MQ=255)                                                                                              aCTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGACGGACAAAGGCAGCTGCCCTTGCACGGTcc                                     >  7:373031/1‑134 (MQ=255)                                                                                                tCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCcaca                                  >  4:344354/1‑135 (MQ=255)                                                                                                   ggTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGc                                                        <  8:81172/110‑1 (MQ=255)                                                                                                   ggTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGc                                                        >  7:81172/1‑110 (MQ=255)                                                                                                       aGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGAtctt                           >  7:287445/1‑135 (MQ=255)                                                                                                            acaATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCttctttt                      >  7:183897/1‑135 (MQ=255)                                                                                                             caATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCttcttttc                     <  3:344354/135‑1 (MQ=255)                                                                                                                 gctgctGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCCCATGATCGTCTTTTCTTg                  >  1:366823/1‑134 (MQ=255)                                                                                                                      tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGc                                                        <  5:5502/91‑1 (MQ=255)                                                                                                                      tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGc                                                        >  6:5502/1‑91 (MQ=255)                                                                                                                      tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAAttt            >  1:316951/1‑135 (MQ=255)                                                                                                                      tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAAttt            >  8:87911/1‑135 (MQ=255)                                                                                                                      tgGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAAtt             <  1:249283/134‑1 (MQ=255)                                                                                                                      tgGAGCGATTCCAAGATTGTCGACAAGCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCCGCTGCCCTTGCCCGGTCCACATGATCTTCGTTTCTTGAAAtat            >  8:83800/1‑133 (MQ=255)                                                                                                                         aGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATtta           <  8:235223/133‑1 (MQ=255)                                                                                                                           cGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTAc       >  5:290872/1‑135 (MQ=255)                                                                                                                             aTTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACtt     <  4:171675/135‑1 (MQ=255)                                                                                                                                 cAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATc  <  7:76485/134‑1 (MQ=255)                                                                                                                                 cAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATc  <  7:159661/134‑1 (MQ=255)                                                                                                                                 cAAGATTGTCGACAAGCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATTCCGTCGGGAGGCCGGACAAAGGCAGCTGACCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATc  <  1:280987/134‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      TTTCAGCCGCTGTGGATAAAACTGTTGTCCAGGGTGCCACCGCCAGCCAATACTCCAACCACCCTCTCCCTGCTGGACAAGGCAAATCGGTTGACTCGGGTCAGCCCACAATGCTGCTGGAGCGATTCCAAGATTGTCGACAATCCTGTTTTTCAAGAATGGGCTGTCAACAATCTGTGGAGATACCGTCGGGAGGCCGGACAAAGGCAGCTGCCCTTGCACGGTCCACATGATCTTCTTTTCTTGAAATTTATTACTTATC  >  ACB122/6101529‑6101790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑