Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,116,246 C→A 8.0% P6P (CCG→CCT)  gbsA ← Betaine aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,116,2460CA 8.0% 136.2 / 3.7 50P6P (CCG→CCT) gbsABetaine aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (26/20);  new base A (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

CCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCGGCGAGAACGCACCGCCGCTGT  >  ACB122/6116113‑6116371
                                                                                                                                     |                                                                                                                             
ccTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCg                                                                                                                              <  6:280861/135‑1 (MQ=255)
      gggCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTa                                                                                                                        <  8:111850/135‑1 (MQ=255)
       ggCGCATTCGGCGTCGGCCACACTGGCTTGGGCGAGGGGCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTAt                                                                                                                       <  8:335911/135‑1 (MQ=255)
              tCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGc                                                                                                                >  1:73235/1‑135 (MQ=255)
                   gTCGGCCACACTGGCTTTGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCatgat                                                                                                           <  7:76137/135‑1 (MQ=255)
                   gTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCatgat                                                                                                           <  7:124643/135‑1 (MQ=255)
                      ggCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATgatgat                                                                                                        <  5:213932/135‑1 (MQ=255)
                             cTGGCGTGGGCGATGGTCGCCACTTGGTGGGTGGATCCTTGGTTGTGGATAGTGAGGTGGTGCTCACTGTCGGTCGACTGGCCGTCGATGAAGTTTTGCGAGTTCGGAGCTATCTGATGCATGTTGATGTCCTCa                                                                                                 <  8:120030/135‑1 (MQ=255)
                                 cTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGgc                                                                                              <  8:23398/134‑1 (MQ=255)
                                   tGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGACGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCatg                                                                                                             <  1:66081/117‑1 (MQ=255)
                                   tGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGACGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCatg                                                                                                             >  2:66081/1‑117 (MQ=255)
                                    gggCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGt                                                                                          >  7:203850/1‑135 (MQ=255)
                                       cGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGa                                                                                       <  4:310427/135‑1 (MQ=255)
                                       cGATGGGCGCCACAAGGTCCGTGGTGCGAGGGTTGTGGATACTCAGCTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGa                                                                                       <  5:371931/135‑1 (MQ=255)
                                             tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGCTGCATGATGATCACCTCAGGCGCGTTGAGCACAg                                                                                 >  1:19841/1‑135 (MQ=255)
                                             tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAg                                                                                 >  1:179574/1‑135 (MQ=255)
                                             tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAg                                                                                 >  7:259147/1‑135 (MQ=255)
                                                   caAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGa                                                                                       <  8:43112/123‑1 (MQ=255)
                                                   caAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGa                                                                                       >  7:43112/1‑123 (MQ=255)
                                                   caAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAg                                                                            >  1:41790/1‑134 (MQ=255)
                                                          cGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGc                                                                    <  4:353257/135‑1 (MQ=255)
                                                           gtggtgCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGc                                                                    <  8:166801/134‑1 (MQ=255)
                                                             ggtgCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGAGGAGc                                                                                                                          >  4:47303/1‑78 (MQ=255)
                                                             ggtgCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGAGGAGc                                                                                                                          >  4:52298/1‑78 (MQ=255)
                                                             ggtgCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGAGGAGc                                                                                                                          <  3:47303/78‑1 (MQ=255)
                                                             ggtgCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGAGGAGc                                                                                                                          <  3:52298/78‑1 (MQ=255)
                                                                gCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAg                                                              <  3:139600/135‑1 (MQ=255)
                                                                     ggTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCAc                                                         <  2:73235/135‑1 (MQ=255)
                                                                            gATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCCGCCCACGtgtg                                                    >  1:182638/1‑133 (MQ=255)
                                                                            gATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCCCTtgtg                                                    >  1:274393/1‑133 (MQ=255)
                                                                            gATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTtgtg                                                    >  8:315755/1‑133 (MQ=255)
                                                                             aTACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTATCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCACGCGCGTTGAGCACAGTCAAGCAATTTGCGGGCCGCCCACATGTGGaa                                                 >  6:235217/1‑135 (MQ=255)
                                                                                           ctCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGAGTTGAGCAAAGTCAAGCAATTGTCTGGCAGCCCACTTGTGGAAGTAGTGAGTCg                                      >  1:68158/1‑132 (MQ=255)
                                                                                            tCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCg                                      >  2:32498/1‑131 (MQ=255)
                                                                                              aCTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCCATTGGCGGGCCGCCCACTTGTGGAAGTAGTGAGTCGGCAgct                                >  4:198964/1‑133 (MQ=255)
                                                                                              aCTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGAt                                >  6:148207/1‑135 (MQ=255)
                                                                                              aCTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGAt                                >  7:130759/1‑135 (MQ=255)
                                                                                              aCTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGAt                                >  6:216038/1‑135 (MQ=255)
                                                                                                   cGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCAt                            >  1:263347/1‑134 (MQ=255)
                                                                                                     gTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGCGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTc                         >  3:265909/1‑135 (MQ=255)
                                                                                                        cACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATGCATTTCCgg                      >  7:146928/1‑135 (MQ=255)
                                                                                                        cACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCgg                      >  8:329883/1‑135 (MQ=255)
                                                                                                        cACTGGCCGTCGATGAAGTTTAGCAAGTGCGGAGCTATCGGATGCATGATGATCAACTCAGGCGCGTTGAGCACAGTCAAGAAATTGGCGGGCAGCCCCCTTGGGGTAGTAGTGAGTCGGCACATCCATTTCCgg                      >  3:17422/1‑135 (MQ=255)
                                                                                                        cACTGGCCGTCGATGAAGTATAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATAGGCGCGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCgg                      >  4:96550/1‑135 (MQ=255)
                                                                                                             gCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCGGCgaga                 >  5:139953/1‑135 (MQ=255)
                                                                                                                  cGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCGGCGAGAACGCa            <  8:259147/135‑1 (MQ=255)
                                                                                                                     tGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGGGAGGCGGCAGATCCATTTCCGGCGAGAACGCAccg         <  1:243962/135‑1 (MQ=255)
                                                                                                                             aGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCGAGTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCGGCGAGAACGCACCGCCGCTGt  <  2:19841/134‑1 (MQ=255)
                                                                                                                               ccAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCGGCGAGAACGCa            >  3:107546/1‑122 (MQ=255)
                                                                                                                               ccAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCGGCGAGAACGCa            <  4:107546/122‑1 (MQ=255)
                                                                                                                                     |                                                                                                                             
CCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGTCAAGCAATTGGCGGGCAGCCCACTTGTGGAAGTAGTGAGTCGGCAGATCCATTTCCGGCGAGAACGCACCGCCGCTGT  >  ACB122/6116113‑6116371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑