Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 172,862 A→G 5.9% L783P (CTC→CCC)  fhuA_1 ← Ferrichrome outer membrane transporter/phagereceptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122172,8620AG 5.9% 219.8 / 2.0 68L783P (CTC→CCC) fhuA_1Ferrichrome outer membrane transporter/phagereceptor
Reads supporting (aligned to +/- strand):  ref base A (22/41);  new base G (2/2);  total (25/43)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCACCGCCCAGCCGCAGGCCATCCAGTGC  >  ACB122/172728‑172992
                                                                                                                                      |                                                                                                                                  
aCTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGa                                                                                                                                    >  3:103238/1‑135 (MQ=255)
     tAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                <  4:116933/134‑1 (MQ=255)
     tAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                <  4:125307/134‑1 (MQ=255)
     tAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                <  5:182528/134‑1 (MQ=255)
     tAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                <  6:218816/134‑1 (MQ=255)
     tAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                <  6:251553/134‑1 (MQ=255)
     tAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                <  6:316380/134‑1 (MQ=255)
       gCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                <  8:209806/132‑1 (MQ=255)
       gCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                >  7:209806/1‑132 (MQ=255)
       gCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGcc                                                                                                                             >  1:163932/1‑135 (MQ=255)
       gCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGc                                                                                                                              >  7:73622/1‑134 (MQ=255)
                 gcgcTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAg                                                                                                                   >  1:299705/1‑135 (MQ=255)
                   gcTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGGGCTTGCCCAGGTCATAGc                                                                                                                  >  1:151847/1‑134 (MQ=255)
                   gcTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGGGCTTGCCCAGGTCATAGc                                                                                                                  >  1:147113/1‑134 (MQ=255)
                     tCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGTCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGa                                                                                                               >  6:321394/1‑135 (MQ=255)
                        caTTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATc                                                                                                             <  2:56822/134‑1 (MQ=255)
                               ctctTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTc                                                                                                     <  8:314608/135‑1 (MQ=255)
                               ctctTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTc                                                                                                     <  8:5783/135‑1 (MQ=255)
                                   tCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGa                                                                                                   <  2:158462/133‑1 (MQ=255)
                                   tCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGa                                                                                                   <  1:87281/133‑1 (MQ=255)
                                   tCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGa                                                                                                   <  1:5618/133‑1 (MQ=255)
                                   tCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGa                                                                                                   <  2:3388/133‑1 (MQ=255)
                                   tCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGa                                                                                                   <  2:65999/133‑1 (MQ=255)
                                   tCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGa                                                                                                   <  4:124975/133‑1 (MQ=255)
                                     aCCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACa                                                                                                <  2:163932/134‑1 (MQ=255)
                                       ccATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAggg                                                                                             <  8:94942/135‑1 (MQ=255)
                                             aGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAg                                                                                        >  8:320036/1‑134 (MQ=255)
                                               cAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGaa                                                                                     >  2:88895/1‑135 (MQ=255)
                                               cAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGaa                                                                                     >  8:313233/1‑135 (MQ=255)
                                                agtagtTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGGGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAg                                                                                    <  2:147113/135‑1 (MQ=255)
                                                agtagtTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGGGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAg                                                                                    <  2:151847/135‑1 (MQ=255)
                                                       cAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCt                                                                              >  2:95575/1‑134 (MQ=255)
                                                                  aTGAGGTGATGTAGCATACATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGCCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGCAAGCCACATACATGCtcattt                                                                  >  4:348086/1‑130 (MQ=255)
                                                                      ggTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCg                                                               <  7:320036/134‑1 (MQ=255)
                                                                      ggTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCg                                                               <  1:88895/134‑1 (MQ=255)
                                                                        tGATGTAGCTTTCATTGGTCAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCAt                                                            <  6:87988/135‑1 (MQ=255)
                                                                        tGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATACACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCAt                                                            <  7:59905/135‑1 (MQ=255)
                                                                          aTGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCg                                                          <  8:213424/135‑1 (MQ=255)
                                                                           tGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                >  3:48031/1‑64 (MQ=255)
                                                                           tGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCtt                                                                                                                                <  4:48031/64‑1 (MQ=255)
                                                                           tGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCg                                                          <  7:361287/134‑1 (MQ=255)
                                                                           tGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCg                                                          <  1:95575/134‑1 (MQ=255)
                                                                           tGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCg                                                          <  1:17321/134‑1 (MQ=255)
                                                                              aGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGAcc                                                       <  2:268486/134‑1 (MQ=255)
                                                                                      ttGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTAc                                              <  7:313233/135‑1 (MQ=255)
                                                                                       tGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGCGCTTGCCCAGGACATAGCCGATCGAGGCGTCGAACAGGGGGTATGCACGCACCTTCCTGCTGTTTTCCCCATCGACCCAGCTCTaac                                             >  2:195226/1‑133 (MQ=255)
                                                                                       tGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTAcc                                             >  7:191817/1‑135 (MQ=255)
                                                                                       tGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTACGCATCGACCCAGCTGTAcc                                             >  5:212730/1‑135 (MQ=255)
                                                                                              gttgttGGCGCTCAAGCGCACATCCACACCCCTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACata                                      <  7:99551/135‑1 (MQ=255)
                                                                                                           aaGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAg                                                                                                          <  8:319787/54‑1 (MQ=255)
                                                                                                           aaGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAg                                                                                                          >  7:319787/1‑54 (MQ=255)
                                                                                                           aaGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCAcc                          <  8:191817/134‑1 (MQ=255)
                                                                                                           aaGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCAcc                          <  3:114550/134‑1 (MQ=255)
                                                                                                           aaGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCACCg                         >  4:105805/1‑135 (MQ=255)
                                                                                                           aaGCGCACATCCACACCCGGCAGGCCGAGCTTGCCCAGGTCATAGCCGTTCGAGGCGTCGAACAGGGTGGAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCAcc                          <  7:106148/134‑1 (MQ=255)
                                                                                                               gcACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCACCGCCCa                     <  2:299705/135‑1 (MQ=255)
                                                                                                                      cacaCCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAg                                                                                                          >  7:192156/1‑43 (MQ=255)
                                                                                                                      cacaCCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAg                                                                                                          <  8:192156/43‑1 (MQ=255)
                                                                                                                       acacCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCACCGCCCAGCCGCAgg             <  3:173875/135‑1 (MQ=255)
                                                                                                                            cGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTCCCCCACATATCGCACCCCACCGCCCAGCCGCAGGCCCTc        >  5:318294/1‑135 (MQ=255)
                                                                                                                              tCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCg                                                          >  4:221208/1‑83 (MQ=255)
                                                                                                                              tCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCg                                                          <  3:221208/83‑1 (MQ=255)
                                                                                                                                aGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTc                                                                            >  6:5472/1‑63 (MQ=255)
                                                                                                                                aGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTc                                                                            <  5:5472/63‑1 (MQ=255)
                                                                                                                                aGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCACCGCCCAGCCGCAGGCCATCCAGt    >  2:121935/1‑135 (MQ=255)
                                                                                                                                 ggCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCACCGCCCAGCCGCAGGCCATCCAGTg   <  3:248193/135‑1 (MQ=255)
                                                                                                                                  gCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTc                                                                                                     >  2:246537/1‑36 (MQ=255)
                                                                                                                                  gCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTc                                                                                                     <  1:246537/36‑1 (MQ=255)
                                                                                                                                  gCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTATCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCACCCCCAAGCCGCACGCCATCCAGTGc  >  7:64612/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                  
ACTGATAGCTGACCGTGGCGCTCACATTGCGCTCTTCACCCATGTAGCAGTAGTTCAGGCTGGCACATGAGGTGATGTAGCTTTCATTGGTAAGGTTGTTGGCGTTCAAGCGCACATCCACACCCGTCAGGCCGAGCTTGCCCAGGTCATAGCCGATCGAGGCGTCGAACAGGGTGTAGGAAGGCACCTTCATGCTGTTTTCCGCATCGACCCAGCTGTACCCCACATATCGCACCCCACCGCCCAGCCGCAGGCCATCCAGTGC  >  ACB122/172728‑172992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑