Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 364,653 C→T 6.6% L362L (CTG→TTG)  stcD_1 → putative N‑methylproline demethylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122364,6530CT 6.6% 187.0 / 2.8 60L362L (CTG→TTG) stcD_1putative N‑methylproline demethylase
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base T (2/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGACATGGTCGGCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGTGGTGGTGGTTGGCGCAGGCCCTGCCGGCATGGAAGCGGC  >  ACB122/364521‑364786
                                                                                                                                    |                                                                                                                                     
gTGGACATGGTCGGCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGcc                                                                                                                                       <  3:359105/133‑1 (MQ=255)
    aCATGGTCGGCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGAtg                                                                                                                                 <  1:380756/135‑1 (MQ=255)
    aCATGGTCGGCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGAt                                                                                                                                  <  4:71353/134‑1 (MQ=255)
       tGGTCGGCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGAtgtgc                                                                                                                              <  8:364605/135‑1 (MQ=255)
       tGGTCGGCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGAtgtgc                                                                                                                              >  5:140049/1‑135 (MQ=255)
         ggcGGCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGAGGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGAtgtgctg                                                                                                                            <  5:57211/133‑1 (MQ=255)
            ggCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGAtgtgctgtgc                                                                                                                         >  8:205081/1‑135 (MQ=255)
                          gcACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAAcgccgc                                                                                                           >  6:134185/1‑135 (MQ=255)
                          gcACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAAcgccgc                                                                                                           >  1:50318/1‑135 (MQ=255)
                          gcACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAAcgccgc                                                                                                           >  4:117928/1‑135 (MQ=255)
                               tGGCCGACCCGCACCTTATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGGGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCt                                                                                                      <  2:216529/135‑1 (MQ=255)
                               tGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCt                                                                                                      <  5:257532/135‑1 (MQ=255)
                               tGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCt                                                                                                      <  6:15656/135‑1 (MQ=255)
                                 gCCGACCCGCACCTTAGCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    <  5:14553/135‑1 (MQ=255)
                                 gCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCTACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    <  3:200678/135‑1 (MQ=255)
                                  ccGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCATAACGCCGCGACCTcc                                                                                                    <  6:94483/134‑1 (MQ=255)
                                  ccGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    <  8:54842/134‑1 (MQ=255)
                                  ccGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    <  8:50240/134‑1 (MQ=255)
                                   cGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    <  2:2651/133‑1 (MQ=255)
                                   cGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    >  1:2651/1‑133 (MQ=255)
                                   cGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATACAGAACGCCGCGACATCCCg                                                                                                  <  6:71598/135‑1 (MQ=255)
                                    gACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    >  6:353592/1‑132 (MQ=255)
                                    gACCCGCACCTGATCGCCAAGAGCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    <  5:353592/132‑1 (MQ=255)
                                           aCCTGATCGCCAAGATCATGATGGGGCAGATCGACCAGATCAATCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACa                                                                                          <  8:104184/135‑1 (MQ=255)
                                           aCCTGATCGCCAAGATCAAGATGGGACAGAGCGCCCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGAGGTGCGGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACa                                                                                          <  4:295451/135‑1 (MQ=255)
                                                 tCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGgt                                                                                     <  7:165320/134‑1 (MQ=255)
                                                   gCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGtgtg                                                                                  >  6:146690/1‑135 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCg                                                                                                  >  8:69411/1‑100 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCg                                                                                                  <  7:69411/100‑1 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACa                                                                                          <  6:377802/108‑1 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACa                                                                                          >  5:377802/1‑108 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGcc                                                                                >  8:299399/1‑118 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGcc                                                                                <  7:9624/118‑1 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGcc                                                                                >  8:9624/1‑118 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGcc                                                                                <  7:299399/118‑1 (MQ=255)
                                                                      aGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGagaaga                                                               >  1:223143/1‑135 (MQ=255)
                                                                           gACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCTTGGAtgtgc                                                                                                                              >  1:117821/1‑67 (MQ=255)
                                                                           gACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCTTGGAtgtgc                                                                                                                              >  1:122848/1‑67 (MQ=255)
                                                                           gACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCTTGGAtgtgc                                                                                                                              <  2:117821/67‑1 (MQ=255)
                                                                           gACCAGATCAAGCAGTCCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCTTGGAtgtgc                                                                                                                              <  2:122848/67‑1 (MQ=255)
                                                                                   cAAGCTGGGCGTCGGTGCCAACTACTGCAGCCCCCGCCAGTCCCAGGGCCTGGATGGGCGGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCaa                                                  <  2:155278/135‑1 (MQ=255)
                                                                                   cAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCaa                                                  <  2:123778/135‑1 (MQ=255)
                                                                                        aGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCa                                             <  3:117928/135‑1 (MQ=255)
                                                                                             gTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAggtg                                        >  4:362076/1‑135 (MQ=255)
                                                                                             gTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAggtg                                        >  4:12584/1‑135 (MQ=255)
                                                                                              tCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGGACATGGGTGTGCCGCACCTCATCGAGAAGACCACCGGCGTCAAGCGCAAggggg                                       >  5:305692/1‑135 (MQ=255)
                                                                                               cGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAggtggt                                      >  8:45442/1‑135 (MQ=255)
                                                                                               cGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAggtggt                                      >  3:245824/1‑135 (MQ=255)
                                                                                                  tGCCAACTACTGCAGCGACCGCAAGTACCTGGGCCTGGAGGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAggtggtggt                                   <  5:98134/135‑1 (MQ=255)
                                                                                                    ccAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAggtggtggtgg                                 >  5:10761/1‑135 (MQ=255)
                                                                                                         tactGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGGGGTGGTGGTTgtc                            >  2:365653/1‑133 (MQ=255)
                                                                                                         tactGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATAGAGAAGACCACCGGCGTCAAGCGCAATGTGGTGGTGGTTGgc                            >  6:43595/1‑135 (MQ=255)
                                                                                                               aTCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAAcgc                                                                                                              >  8:245323/1‑47 (MQ=255)
                                                                                                               aTCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAAcgc                                                                                                              <  7:245323/47‑1 (MQ=255)
                                                                                                                 cGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    >  2:302778/1‑55 (MQ=255)
                                                                                                                 cGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTcc                                                                                                    <  1:302778/55‑1 (MQ=255)
                                                                                                                      gCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGCCCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGTGGTGGTGGTTGGCGCAGGCCCTGCCg               >  5:87661/1‑135 (MQ=255)
                                                                                                                      gCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGTGGTGGTGGTTGGCGCAGGCCCTGCCg               >  2:19546/1‑135 (MQ=255)
                                                                                                                      gCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGTGGTGGTGGTTGGCGCAGGCCCTGCCg               >  8:138083/1‑135 (MQ=255)
                                                                                                                      gCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGTGGTGGTGGTTGGCGCAGGCCCTGCCg               >  6:234412/1‑135 (MQ=255)
                                                                                                                      gCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGTGGTGGGGGTTGGCGCAGGCCCTGCCg               >  3:287037/1‑135 (MQ=255)
                                                                                                                                   ccTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGTGGTGGTGGTTGGCGCAGGCCCTGCCGGCATGGAAGCGGc  >  7:28894/1‑135 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GTGGACATGGTCGGCATGACCCGTGCGCACATGGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAGCAGTGCGTCGGTGCCAACTACTGCATCGACCGCCAGTACCAGGGCCTGGATGTGCTGTGCATCCAGAACGCCGCGACCTCCCGTGAGTACATGGGTGTGCCGCACATCATCGAGAAGACCACCGGCGTCAAGCGCAAGGTGGTGGTGGTTGGCGCAGGCCCTGCCGGCATGGAAGCGGC  >  ACB122/364521‑364786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑