Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 478,881 A→C 27.4% V35G (GTC→GGC)  apaH_1 ← Bis(5'‑nucleosyl)‑tetraphosphatase, symmetrical

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122478,8810AC27.4% 57.8 / 3.4 52V35G (GTC→GGC) apaH_1Bis(5'‑nucleosyl)‑tetraphosphatase, symmetrical
Reads supporting (aligned to +/- strand):  ref base A (22/14);  new base C (14/2);  total (36/16)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGACGTTGTGCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCG  >  ACB122/478751‑479013
                                                                                                                                  |                                                                                                                                    
cgcGACGTTGTGCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAg                                                                                                                                  <  3:155508/133‑1 (MQ=255)
   gACGTTGTGCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACTAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGcc                                                                                                                                >  1:272294/1‑134 (MQ=255)
   gACGTTGTGCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCCGccc                                                                                                                               >  6:277926/1‑134 (MQ=255)
    aCGTTGTGCCAGGCGCCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCcac                                                                                                                              <  3:188488/135‑1 (MQ=255)
             cAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCg                                                                                                                     >  7:284781/1‑135 (MQ=255)
                  ggcCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGCcggc                                                                                                                >  4:87894/1‑135 (MQ=255)
                   gcCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGCCCAGGTCGCCGCCCAGCCACAGGCGGGCGAcgggg                                                                                                               >  1:62339/1‑133 (MQ=255)
                       gcaggtgcaggtCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCACGTCGCCGACCAGCC‑CAGGCCGTCGACGGCgggggt                                                                                                          >  3:162325/1‑133 (MQ=255)
                                        ttGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGCCCAGGTCGCCGCCCCGCCCCAGGCGGGCGACGGCGGGGGTGGGACGGACGCGCTCg                                                                                          >  5:273251/1‑135 (MQ=255)
                                        ttGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACAGGCGGTCGACGGCGGGGTTGCAACTGACGCGAt                                                                                            >  4:257491/1‑133 (MQ=255)
                                        ttGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGAt                                                                                            >  7:294274/1‑133 (MQ=255)
                                        ttGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGCCGGCGGGGTTGAAACGGCCGCGATCg                                                                                          >  7:327963/1‑135 (MQ=255)
                                         tGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGCCCAGGTCGCCGCCCAGCCCCAGGCGGGCGCCGGCGGGGTTGAAACTGCCGCGCTCGa                                                                                         >  5:44520/1‑135 (MQ=255)
                                         tGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGa                                                                                         >  3:128934/1‑135 (MQ=255)
                                           cccAGCACGCAGACACGCGAATGCCGGCGCGCGTACAGGACGCCCGGGGTTACCGGTGACTCGGGGCCGGGGGTGCCCAGGGCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGc                                                                                       <  2:66719/135‑1 (MQ=255)
                                              aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCCCCCCAGGCGGTCGACGGCGGGGTTGACACTGACGCGCTCGCGCCgg                                                                                    >  2:163386/1‑135 (MQ=255)
                                              aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAg                                                                                     >  8:3423/1‑134 (MQ=255)
                                                   gctgACCAGTGACTGCGGTATCTAGAAAAGAAAGCCATGGGTTCCCTGTGACTGGGGGCCGGGGTTTACCCGGGCGCCGACCAGCCCCGGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAttt                                                                               <  8:260690/132‑1 (MQ=255)
                                                   gCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCCCCGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAttt                                                                               >  3:367978/1‑135 (MQ=255)
                                                   gCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCCGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAtt                                                                                >  3:264109/1‑134 (MQ=255)
                                                   gCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGCCGGCGGGGGTGAAACTGACGCGATCGAGCAGGCAtt                                                                                >  6:196070/1‑134 (MQ=255)
                                                   gCAGACCAGTGAATGACGGAGCGATTACAGCAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGGCGCCGACCAGCCACAGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCAttt                                                                               <  3:183624/135‑1 (MQ=255)
                                                      gACCTGTGATTGACGGATCGAGTACAGGACGCTCCGGGTTGCCAGTGAGTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAg                                                                            <  8:125082/135‑1 (MQ=255)
                                                        ccAGTGAATGACTGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCCACCAGCCACACGCGGTCGACGGCGGGGTTGAAACTGACGAGATAGAGCAGGCAGTTGAGCg                                                                          >  7:77021/1‑135 (MQ=255)
                                                         cAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGTGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCgg                                                                         >  4:148075/1‑135 (MQ=255)
                                                         cAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACACGCGGTCGCCGGCCGGGTTGAAACTGACTCGAACGAGCAGGCATTTGAGCgg                                                                         >  2:75364/1‑135 (MQ=255)
                                                          aGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCCCAGGCGGTCGCCGGCGGGGGTGAAACTGACGCCCTCGAGCACGCATTTGAGCGGc                                                                        >  5:266889/1‑135 (MQ=255)
                                                             gAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGCCTCGGGGCCGCGGTTGACCAGGTCGCCGACCCGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGAGCGAGCAGGCATTTGCGCGGCTgc                                                                     >  2:468/1‑135 (MQ=255)
                                                                    ggATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGc                                                              >  1:504/1‑135 (MQ=255)
                                                                    ggATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCCGCCCCCGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGCGCCGGCATTTGAGCGGCTGCAGGCAGc                                                              >  1:224311/1‑135 (MQ=255)
                                                                    ggATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCCGCCCCAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGc                                                              >  7:7093/1‑135 (MQ=255)
                                                                    ggATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCCCCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGc                                                              >  6:266287/1‑135 (MQ=255)
                                                                         gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGc                                                         >  5:360918/1‑135 (MQ=255)
                                                                              ccgcaaGCGCAGGGTTGCCAGTGACTGGGGGCCGCGGTGGACCAGGGCGCCGACCAGCCACCGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTc                                                    <  7:254443/131‑1 (MQ=255)
                                                                                ggACGCGCCGGGTTGCCTGGGCCGGGGGGCCGCGGTTCACCTGGGCGCCGACCAGCCACTGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCAc                                                  <  1:50036/135‑1 (MQ=255)
                                                                                 gAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCAcc                                                 <  4:128934/135‑1 (MQ=255)
                                                                                      gccgggTTTCCTGTGACGCGGGGCCGGGGTGGCCCCGGGCGCCGACCCGCCACCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACgg                                            <  6:325028/132‑1 (MQ=255)
                                                                                       cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGTTCGCGCAGGGATTTGAGCGGCTGCAGGCCGCCCTGCAGGTCCCCGCCggg                                           >  8:141365/1‑134 (MQ=255)
                                                                                        aGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGGCCCCGACGGCg                                          >  4:57516/1‑135 (MQ=255)
                                                                                            tttCCAGTGACTCGGGGCCGCGGTTGCCCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGTAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGGCACCGACGGCGTAgg                                      >  4:218623/1‑135 (MQ=255)
                                                                                            tttCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAgg                                      >  4:321741/1‑135 (MQ=255)
                                                                                             tcccAGTGACGGGGGGCCGCGGTTGCCCAGGGCCCCGACCCGCCACCGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGt                                     <  8:17643/133‑1 (MQ=255)
                                                                                              tCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGGAGGTg                                    >  5:289552/1‑135 (MQ=255)
                                                                                                  gTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGACGGGGGGGGTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCa                                >  1:224588/1‑135 (MQ=255)
                                                                                                  gTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCACCCACAGGCGGTCGACGGCCGGGTTGAAACTGACGCGATCGAGCACGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCa                                >  6:127922/1‑135 (MQ=255)
                                                                                                       tgggggCCGGGGAGGCCCGGGGCGCCGCCCCGCCCCGGGGGGGCGACGGAGGGGTTGAAACGGACGCGATCGAGAAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGt                           <  1:163386/133‑1 (MQ=255)
                                                                                                       gcGGGGCGGCGGTTGCCCTGGGCGCCGCCCACCCACCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGt                           <  5:118123/134‑1 (MQ=255)
                                                                                                       gcGGGGCCGCGGGTGACCCGGGCGCCGACCAGCCACCGGGGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGt                           <  8:327963/134‑1 (MQ=255)
                                                                                                        cGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACCGGCGTTCGACCGCGGGGTTGAAAATGAAGCCATCGAGCACGCATATTAGCGGCAGCATGCAGCCCTGCAGGTAACAGACGGCCTAGGTGGCCATCAGt                           >  8:39700/1‑134 (MQ=255)
                                                                                                                              gCCGACCAGCCCCAGGCGGTCGCCGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCCCCGCCGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGc    >  7:113329/1‑135 (MQ=255)
                                                                                                                                cGACCAGCCCCCGGCGGGCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCg  <  3:36464/135‑1 (MQ=255)
                                                                                                                                cGACCAGCCACCGGGGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCg  <  8:52208/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CTCGACGTTGTGCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGACGGCGGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCCG  >  ACB122/478751‑479013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑