Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 537,063 C→T 6.4% D751D (GAC→GAT)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122537,0630CT 6.4% 246.4 / 5.6 78D751D (GAC→GAT) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (31/42);  new base T (2/3);  total (33/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

TCGACCGCGAAGGCAAGGAAGTCGAGCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGCTAACTCCGGTTACCT  >  ACB122/536930‑537197
                                                                                                                                     |                                                                                                                                      
tCGACCGCGAAGGCAAGGAAGTCGAGCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACg                                                                                                                                       <  2:116690/135‑1 (MQ=255)
   aCCGCGAAGGCAAGGAAGTCGAGCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGtt                                                                                                                                    >  3:1612/1‑135 (MQ=255)
   aCCGCGAAGGCAAGGAAGTCGAGCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGtt                                                                                                                                    >  6:348486/1‑135 (MQ=255)
    ccGCGAAGGCAAGGAAGGCGAGCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTc                                                                                                                                   <  4:286624/135‑1 (MQ=255)
       cgAAGGCAAGGAAGTCGAGCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCcat                                                                                                                                <  3:153953/135‑1 (MQ=255)
         aaGGCAAGGAAGTCGAGCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCcatca                                                                                                                              <  8:29694/135‑1 (MQ=255)
                         gCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCACGCCGGACGGTTCCATCATCGAGACGCCGATCAc                                                                                                              >  7:373230/1‑135 (MQ=255)
                            agagTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTGCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGGCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACcgc                                                                                                           <  1:272439/135‑1 (MQ=255)
                               gTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGaa                                                                                                        <  1:261224/135‑1 (MQ=255)
                               gTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGGCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGaa                                                                                                        <  5:348486/135‑1 (MQ=255)
                                  cTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCCAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACtt                                                                                                     >  2:63300/1‑135 (MQ=255)
                                  cTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACtt                                                                                                     >  8:280840/1‑135 (MQ=255)
                                     cAACTCGATGTACATGATGGCTGACTCGGGTGCTCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGACGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACttacc                                                                                                  >  2:99331/1‑132 (MQ=255)
                                     cAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCg                                                                                                  >  7:203297/1‑135 (MQ=255)
                                     cAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCg                                                                                                  >  5:272693/1‑135 (MQ=255)
                                      aaCTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTcc                                                                                                   <  5:133506/133‑1 (MQ=255)
                                      aaCTCGATGTAACTGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCg                                                                                                  <  6:106862/134‑1 (MQ=255)
                                       aCTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGt                                                                                                 <  7:56929/134‑1 (MQ=255)
                                        cTCGATGTACATGATGTCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGATGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGt                                                                                                 >  8:304213/1‑133 (MQ=255)
                                         tCGATGAACACGATGGCGGACTCGGGTGCGCGGGGGTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGCTGGCCAAGCCGGACGGGTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGaa                                                                                              <  7:342287/135‑1 (MQ=255)
                                           gATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAgg                                                                                            >  6:118024/1‑135 (MQ=255)
                                             tGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTc                                                                                          >  5:156345/1‑135 (MQ=255)
                                             tGTACATGATGGCTGACTCGGGTGCGCGGGGGGCCGCAGCCCAGATCCGTCAGCTGGCCGGTCTGCGGGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTTAAGGTc                                                                                          <  2:366540/135‑1 (MQ=255)
                                              gTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTc                                                                                          >  7:19261/1‑134 (MQ=255)
                                                aCATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGa                                                                                       <  7:258311/135‑1 (MQ=255)
                                                  atgatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGa                                                                                       <  2:64922/133‑1 (MQ=255)
                                                  atgatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGa                                                                                       <  2:321583/133‑1 (MQ=255)
                                                      tgGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgta                                                                                  <  4:1612/134‑1 (MQ=255)
                                                      tgGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGGGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgta                                                                                  <  8:162322/134‑1 (MQ=255)
                                                       ggCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtact                                                                                >  3:181821/1‑135 (MQ=255)
                                                          tGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtacttca                                                                             >  6:265436/1‑135 (MQ=255)
                                                          tGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCCAGACGCCGATCACCGCGAACTTCCGTGGAGGTCTGAGCgtacttca                                                                             >  1:380162/1‑135 (MQ=255)
                                                           gACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtacttcag                                                                            <  5:118024/135‑1 (MQ=255)
                                                            aCTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtacttcagt                                                                           >  4:348114/1‑135 (MQ=255)
                                                             cTCGGGTGGGCGGGGTTCCGAAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGCTGCCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtacttcagta                                                                          <  2:215555/135‑1 (MQ=255)
                                                             cTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtacttcag                                                                            <  4:233005/133‑1 (MQ=255)
                                                             cTCGGGGGCGGGTGGTTCCGCAGCCCAGATCGGGCATGTGGCCGGTATGCGTGGGCGGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtacttcagta                                                                          <  5:18728/135‑1 (MQ=255)
                                                               cGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCCCCGCGAACTTCCGTGAAGGTCGGAGCgtacttcagtact                                                                        >  7:323602/1‑135 (MQ=255)
                                                                 ggTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtacttcagtacttc                                                                      >  1:119236/1‑135 (MQ=255)
                                                                        ggggTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGa                                                               >  8:132276/1‑135 (MQ=255)
                                                                         gggTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGAc                                                              <  3:339106/135‑1 (MQ=255)
                                                                           gTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTc                                                            <  3:219419/135‑1 (MQ=255)
                                                                           gTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTc                                                            <  8:19261/135‑1 (MQ=255)
                                                                           gTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTc                                                            <  3:121303/135‑1 (MQ=255)
                                                                             tacGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGTACTTCCGTGAATGTGTGAGCGTTCTTCAGTACTTCATCTCGACTCAc                                                          <  4:237009/133‑1 (MQ=255)
                                                                                    cccAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTc                                                   <  7:265272/135‑1 (MQ=255)
                                                                                    cccAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTc                                                   <  7:132276/135‑1 (MQ=255)
                                                                                     gcAGATCCGTCAGGTGGCCGGTATGCGGGGTCTGATGGCCAAGCCGGTCGGTTCCATCATGGAGACGCCGATCACCGCGAAGTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCTTCTCGACTCACGGTGCTCg                                                  <  8:321826/134‑1 (MQ=255)
                                                                                     ccAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCg                                                  <  2:119236/135‑1 (MQ=255)
                                                                                       aGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTc                                                   >  7:274729/1‑132 (MQ=255)
                                                                                       aGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTc                                                   <  8:274729/132‑1 (MQ=255)
                                                                                       aGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCg                                                  >  6:161474/1‑133 (MQ=255)
                                                                                         aTCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACTGTTACATCATAGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGGACTTCAGTACTTCATCTCGACTCACGGGGCTCGTaat                                              >  3:206533/1‑134 (MQ=255)
                                                                                         aTCCGTCAGCTGGCCGGTATGCGGGGTCTGAGGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAg                                              <  2:349128/135‑1 (MQ=255)
                                                                                                 gCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGa                                                                                       <  3:61703/86‑1 (MQ=255)
                                                                                                 gCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGa                                                                                       >  4:61703/1‑86 (MQ=255)
                                                                                                 gCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCAtct                                                                  <  8:17339/107‑1 (MQ=255)
                                                                                                 gCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCAtct                                                                  >  7:17339/1‑107 (MQ=255)
                                                                                                 gCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGc                                      >  3:35472/1‑135 (MQ=255)
                                                                                                 gCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGc                                      >  5:371067/1‑135 (MQ=255)
                                                                                                 gCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCACGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGc                                      >  6:4210/1‑135 (MQ=255)
                                                                                                     gCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtact                                                                                <  8:305621/89‑1 (MQ=255)
                                                                                                     gCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCgtact                                                                                >  7:305621/1‑89 (MQ=255)
                                                                                                        ggTATGCTTGGTGTGATGGCCAAGCCGGACTGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATAcc                               <  1:99331/135‑1 (MQ=255)
                                                                                                        ggTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATAcc                               <  6:272693/135‑1 (MQ=255)
                                                                                                              cGTGGGCGGAGGGCCAAGCCGGCCGGTTCCATCAGCGAGCCGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTg                         <  4:5083/135‑1 (MQ=255)
                                                                                                                   tCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTg                         <  2:259206/130‑1 (MQ=255)
                                                                                                                   tCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTg                         >  1:259206/1‑130 (MQ=255)
                                                                                                                       aTGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGc                 >  3:212361/1‑134 (MQ=255)
                                                                                                                        tGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGc                 <  6:371067/133‑1 (MQ=255)
                                                                                                                        tGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGc                 <  6:142218/133‑1 (MQ=255)
                                                                                                                        tGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGc                 <  5:328598/133‑1 (MQ=255)
                                                                                                                           ccAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTGATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGCTAACt            <  8:68606/135‑1 (MQ=255)
                                                                                                                           ccAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGCTAACt            >  4:356388/1‑135 (MQ=255)
                                                                                                                             aaGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGCTAACTcc          <  8:90715/135‑1 (MQ=255)
                                                                                                                                ccGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                 <  7:70914/125‑1 (MQ=255)
                                                                                                                                ccGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                 >  8:70914/1‑125 (MQ=255)
                                                                                                                                ccGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGCTAACTCCgg        >  4:132645/1‑134 (MQ=255)
                                                                                                                                     cGGTTCCTTCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGCTAACTCCGGTTACCt  <  5:230367/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCGACCGCGAAGGCAAGGAAGTCGAGCAAGAGTCCTTCAACTCGATGTACATGATGGCTGACTCGGGTGCGCGGGGTTCCGCAGCCCAGATCCGTCAGCTGGCCGGTATGCGTGGTCTGATGGCCAAGCCGGACGGTTCCATCATCGAGACGCCGATCACCGCGAACTTCCGTGAAGGTCTGAGCGTACTTCAGTACTTCATCTCGACTCACGGTGCTCGTAAGGGTCTGGCGGATACCGCACTGAAAACCGCTAACTCCGGTTACCT  >  ACB122/536930‑537197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑