Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 603,705 C→T 5.2% R36H (CGC→CAC)  btuB_1 ← Vitamin B12 transporter BtuB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122603,7050CT 5.2% 221.9 / 2.9 77R36H (CGC→CAC) btuB_1Vitamin B12 transporter BtuB
Reads supporting (aligned to +/- strand):  ref base C (28/45);  new base T (2/2);  total (30/47)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.38e-01

ACCGGTAGGTGCCACTTGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTCCACAGC  >  ACB122/603572‑603837
                                                                                                                                     |                                                                                                                                    
aCCGGTAGGTGCCACTTGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGAGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTggcg                                                                                                                                     >  2:151032/1‑135 (MQ=255)
  cGGTAGGTGCCACTTGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTggcggc                                                                                                                                   >  3:328298/1‑135 (MQ=255)
          gCCACTTGCACGCCCGGCCCGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATCTCGGTGCGGGTGAAAACCGTGTTGTCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTg                                                                                                                           >  1:318740/1‑135 (MQ=255)
           ccACTTGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTCCGCGATTCGACCTGGCGGCTGGCGCTGa                                                                                                                          >  3:303326/1‑135 (MQ=255)
           ccACTTGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGa                                                                                                                          >  6:330852/1‑135 (MQ=255)
              cTTGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTCCGCGATTCGACCTGGCGGCTGGCGCTGATCa                                                                                                                       >  7:236721/1‑135 (MQ=255)
               ttGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCa                                                                                                                       >  6:153678/1‑134 (MQ=255)
                tGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGc                                                                                                                     >  4:164065/1‑135 (MQ=255)
                 gCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCa                                                                                                                    >  1:208635/1‑135 (MQ=255)
                   aCGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACg                                                                                                                  <  5:369465/135‑1 (MQ=255)
                     gCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGGAGGCGGTCGATGGCGGTGCGGGTGAAAACCGTGTTGGCTGAACTGGTGGCGGTACGCGATGCGACCTGGCGGCTGGCGCTGATCAGCACGTc                                                                                                                <  1:151032/135‑1 (MQ=255)
                     gCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGAGGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGGGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTc                                                                                                                <  3:207610/135‑1 (MQ=255)
                                gggAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGa                                                                                                     <  8:314013/135‑1 (MQ=255)
                                gggAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGa                                                                                                     <  5:278639/135‑1 (MQ=255)
                                gggAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTGGGCTGCACTGGGGGTGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGa                                                                                                     <  2:297535/135‑1 (MQ=255)
                                  gagaGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCAGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAgg                                                                                                   <  4:31996/135‑1 (MQ=255)
                                      gCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGcgt                                                                                               >  6:22410/1‑135 (MQ=255)
                                               tGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCt                                                                                      >  7:94349/1‑135 (MQ=255)
                                                  cGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAg                                                                                   >  6:218350/1‑135 (MQ=255)
                                                  cGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTCAGGGCGTCGTCGCGCTCAg                                                                                   >  3:314501/1‑135 (MQ=255)
                                                       gcgggTTGCAGGGGGTCGAGGTCGGTGCGGGGGAAACCCGGGTTGGCTGCACGGGGGGCGGTACGCGATTCGACCTGGCGGCGGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGGCGTCGCGCTCAGCGGcc                                                                              <  6:57889/133‑1 (MQ=255)
                                                       gAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACg                                                                                                                  >  1:328217/1‑99 (MQ=255)
                                                       gAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACg                                                                                                                  <  2:328217/99‑1 (MQ=255)
                                                       gAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCgg                                                                                >  8:273803/1‑133 (MQ=255)
                                                       gAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGcc                                                                              <  8:178945/135‑1 (MQ=255)
                                                         gggTTGCAGGCGGGCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAg                                                                            <  2:162543/135‑1 (MQ=255)
                                                           gTTGCAGGCGGGCGATGTCGGTGCGGGGGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTC‑GGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAg                                                                         <  7:197117/135‑1 (MQ=255)
                                                            ttGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGcc                                                                              <  1:148466/130‑1 (MQ=255)
                                                            ttGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGcc                                                                              >  2:148466/1‑130 (MQ=255)
                                                                aGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGAATCGACCTGGCGGCTGGCGCTGATCAGCACGGCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAAt                                                                     >  6:268414/1‑135 (MQ=255)
                                                                  gCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGA‑GGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTa                                                                  <  3:164065/135‑1 (MQ=255)
                                                                      tCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAggg                                                               <  7:320386/135‑1 (MQ=255)
                                                                      tCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAggg                                                               <  7:273803/135‑1 (MQ=255)
                                                                      tCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGGGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAggg                                                               <  6:75109/135‑1 (MQ=255)
                                                                        gATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGc                                                             >  5:150064/1‑135 (MQ=255)
                                                                        gATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGc                                                             >  5:129008/1‑135 (MQ=255)
                                                                             cGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACaga                                                        <  6:199874/135‑1 (MQ=255)
                                                                                       gAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGt                                              >  2:2339/1‑135 (MQ=255)
                                                                                       gAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGt                                              >  5:221375/1‑135 (MQ=255)
                                                                                       gAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGt                                              >  2:301757/1‑135 (MQ=255)
                                                                                         aaaCCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTAc                                            <  5:153678/135‑1 (MQ=255)
                                                                                         aaaCCGTGTTGGCTGCACTGGGGGCGGTACGCGTTTCGACCTGGCGGCTGGCTCTGAGCAGCACGTCGGGGAGTGTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGTCACAGAGAATCGTGGTAc                                            <  6:56948/135‑1 (MQ=255)
                                                                                             cGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGc                                                             <  1:309140/114‑1 (MQ=255)
                                                                                             cGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGc                                                             >  2:309140/1‑114 (MQ=255)
                                                                                             cGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAgg                                         >  1:2214/1‑134 (MQ=255)
                                                                                               tgtTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTa                                      <  7:109956/135‑1 (MQ=255)
                                                                                                    gCTGCACTGGTGGCGGTACGCGATGCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCGCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAAtttt                                 <  8:9877/135‑1 (MQ=255)
                                                                                                        cACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGcc                                                                              >  3:313050/1‑86 (MQ=255)
                                                                                                        cACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGcc                                                                              <  4:313050/86‑1 (MQ=255)
                                                                                                         aCTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCAt                            <  6:150064/135‑1 (MQ=255)
                                                                                                         aCTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCAt                            >  4:196795/1‑135 (MQ=255)
                                                                                                          cTGTTGGAGGTACGAGATTCGACCTGGCGGATGGCGCTTATAAGCAAGTCGGGGAGTTTTATGGCGTATTCGAGCTCAGCGGCCAGAAGGAATGTAAGGGCACATAGAATCGTGGTAAACGCTAATTTTTTAAtt                           >  8:243481/1‑134 (MQ=255)
                                                                                                           tggtggGGGTACGCGATTCGACCTGGCGGCTGTCGCTGATCAGCACGTGGGTGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACATAGAATCGTGGTACAGGCTAATTTTTTCATgg                          <  7:255001/135‑1 (MQ=255)
                                                                                                           tggtggCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATgg                          <  1:301757/135‑1 (MQ=255)
                                                                                                           tggtggCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATgg                          <  4:328967/135‑1 (MQ=255)
                                                                                                           tggtggCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATgg                          <  1:85348/135‑1 (MQ=255)
                                                                                                           tggtggCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATgg                          <  3:301665/135‑1 (MQ=255)
                                                                                                                  ggTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTc                   <  5:22410/135‑1 (MQ=255)
                                                                                                                   gTACGCGATTCGACCTGGTGGCTGGCGCTGATCNGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTc                   >  3:323167/1‑134 (MQ=255)
                                                                                                                   gTACGCGATTCGACCTGGTGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTc                   >  3:317717/1‑134 (MQ=255)
                                                                                                                     aCGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTcc                  >  3:24199/1‑133 (MQ=255)
                                                                                                                     aCGCGAGTCGACCTGTCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGGCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCaa                <  4:199941/135‑1 (MQ=255)
                                                                                                                       gcgATTCGACCTGGTGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAg              <  4:317717/135‑1 (MQ=255)
                                                                                                                       gcgATTCGACCTGGTGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAg              <  4:323167/135‑1 (MQ=255)
                                                                                                                       gcgATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTTGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAg              <  3:188420/135‑1 (MQ=255)
                                                                                                                       gcgATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAg              <  7:63177/135‑1 (MQ=255)
                                                                                                                       gcgATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGAGTCGTCGCGCTCAGCGGCCAGAATGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAg              <  4:228093/135‑1 (MQ=255)
                                                                                                                        cgATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAgg             >  4:52909/1‑135 (MQ=255)
                                                                                                                           ttCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAg                                                                                                          >  2:346896/1‑39 (MQ=255)
                                                                                                                           ttCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAg                                                                                                          <  1:346896/39‑1 (MQ=255)
                                                                                                                            tCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGttt         <  5:218350/135‑1 (MQ=255)
                                                                                                                            tCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGttt         <  7:263419/135‑1 (MQ=255)
                                                                                                                            tCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGttt         <  6:200987/135‑1 (MQ=255)
                                                                                                                              gtccTGGCGGCTGGCGCTGATCAGAACGTCGGGGAGTTTGAGGGCGTCGTCGCGCGCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTTGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTcc       <  2:318740/133‑1 (MQ=255)
                                                                                                                              gACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTcc       <  4:328298/135‑1 (MQ=255)
                                                                                                                                 cTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTCcaca    <  7:74764/135‑1 (MQ=255)
                                                                                                                                 cTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTCcaca    <  7:87552/135‑1 (MQ=255)
                                                                                                                                 cTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTCcaca    <  2:281713/135‑1 (MQ=255)
                                                                                                                                 cTGGCGGCTGGCGCTGATCAGCACGGCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTCcaca    <  2:366503/135‑1 (MQ=255)
                                                                                                                                   ggcggcTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTCCACAGc  <  2:184290/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
ACCGGTAGGTGCCACTTGCACGCCCGGCACGCGGGAGAGCAAGTCGGTGACGCTGGAGGGTTGCAGGCGGTCGATGTCGGTGCGGGTGAAAACCGTGTTGGCTGCACTGGTGGCGGTACGCGATTCGACCTGGCGGCTGGCGCTGATCAGCACGTCGGGGAGTTTGAGGGCGTCGTCGCGCTCAGCGGCCAGAAGGAATGTAGGGGCACAGAGAATCGTGGTACAGGCTAATTTTTTCATGGGCAGTTCCAAAGGGTTTCCACAGC  >  ACB122/603572‑603837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑