Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	879,368	C→T	5.7%	R743R (CGC→CGT)	malT →	HTH‑type transcriptional regulator MalT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	879,368	0	C	T	5.7%	224.9 / 3.0	72	R743R (CGC→CGT)	malT	HTH‑type transcriptional regulator MalT
Reads supporting (aligned to +/- strand):  ref base C (27/40);  new base T (2/2);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

GCCTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAGGCAAGGAAGGGCAGGCCGCGCAGTTGCTGCCA  >  ACB122/879235‑879494                                                                                                                                      |                                                                                                                               gcCTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGcc                                                                                                                               >  1:134952/1‑135 (MQ=255) gcCTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGcc                                                                                                                               >  5:207979/1‑135 (MQ=255) gcCTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGcc                                                                                                                               >  6:131905/1‑135 (MQ=255) gcCTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGcc                                                                                                                               >  6:200852/1‑135 (MQ=255) gcCTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGcc                                                                                                                               >  6:229812/1‑135 (MQ=255) gcCTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGcc                                                                                                                               >  8:172171/1‑135 (MQ=255)  ccTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCTCATTGCGTGGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCt                                                                                                                              <  2:90422/135‑1 (MQ=255)  ccTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCt                                                                                                                              <  3:88294/135‑1 (MQ=255)      ggCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCa                                                                                                                          >  2:325523/1‑135 (MQ=255)      ggCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCa                                                                                                                          >  2:97657/1‑135 (MQ=255)      ggCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCa                                                                                                                          <  1:91249/135‑1 (MQ=255)       gCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCa                                                                                                                          <  8:179140/134‑1 (MQ=255)        ccAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTACATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGAAGATGCCGTGCAACCCCTGGCATg                                                                                                                        >  5:279223/1‑135 (MQ=255)           gACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCt                                                                                                                     <  8:127110/135‑1 (MQ=255)               tATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGt                                                                                                                  >  1:147708/1‑134 (MQ=255)               tATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGt                                                                                                                  >  5:138428/1‑134 (MQ=255)                    cggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTTCACATTTCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAgcg                                                                                                            >  6:230864/1‑135 (MQ=255)                    cggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCACCGCCTGGCATGCCTGGTAAAgcg                                                                                                            >  2:135024/1‑135 (MQ=255)                    cggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAgcg                                                                                                            >  5:106310/1‑135 (MQ=255)                    cggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAgcg                                                                                                            >  4:94290/1‑135 (MQ=255)                    cggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAgcg                                                                                                            <  4:281389/135‑1 (MQ=255)                    cggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAg                                                                                                              >  8:184381/1‑133 (MQ=255)                     ggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAgcgc                                                                                                           <  7:178826/135‑1 (MQ=255)                     ggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAgcgc                                                                                                           <  4:36159/135‑1 (MQ=255)                     ggcgAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAgcgc                                                                                                           <  4:137241/135‑1 (MQ=255)                         agcagcCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCgg                                                                                                         >  6:345379/1‑133 (MQ=255)                         agcagcCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCgg                                                                                                         >  7:293183/1‑133 (MQ=255)                          gcagcCGTCGGCGGCACCGGAGTGCCATCCGCTGCAGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCggcca                                                                                                      >  6:317829/1‑135 (MQ=255)                          gcagcCGGCGGCGGCACCGGAGTGCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTTGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCggcca                                                                                                      <  8:311117/135‑1 (MQ=255)                           cagcCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCggccag                                                                                                     >  1:381618/1‑135 (MQ=255)                                  cggcggcACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTg                                                                                              <  2:217197/135‑1 (MQ=255)                                      ggcACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTggcgg                                                                                          <  3:82822/135‑1 (MQ=255)                                      ggcACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTggcgg                                                                                          <  4:156975/135‑1 (MQ=255)                                      ggcACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTggcgg                                                                                          <  2:134952/135‑1 (MQ=255)                                      ggcACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTggcgg                                                                                          <  3:158529/135‑1 (MQ=255)                                           cGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCatga                                                                                     >  1:136734/1‑135 (MQ=255)                                               gTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCt                                                                                 <  6:73607/135‑1 (MQ=255)                                               gTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCt                                                                                 <  6:141473/135‑1 (MQ=255)                                                          tgctgcCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGAGGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAgcg                                                                      <  7:107994/135‑1 (MQ=255)                                                          tgctgcCGGTGCACATTGCGTTGCAGCTGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCTGGACAGTGGCGGCATGATGCTTACCGTCAgcg                                                                      <  6:269500/135‑1 (MQ=255)                                                             tgcCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGccc                                                                   >  3:299056/1‑135 (MQ=255)                                                               ccGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTATCGACGATGCCGTGCTACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCa                                                                         <  2:172811/127‑1 (MQ=255)                                                               ccGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCa                                                                         >  1:172811/1‑127 (MQ=255)                                                                 gTTGGACATTGCGTTGCAGCAGGCAGCGCTCGATCGCTTCGAGTTGCGTAGCGACGATGCCGTGCAACGGCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAg                                                               <  6:279223/135‑1 (MQ=255)                                                                  ttGCTCATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAg                                                               >  3:361311/1‑134 (MQ=255)                                                                  ttGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGc                                                              >  1:127832/1‑135 (MQ=255)                                                                      acaTTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGTCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAAt                                                          <  3:149649/135‑1 (MQ=255)                                                                      acaTTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCaa                                                           <  6:241766/134‑1 (MQ=255)                                                                       caTTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATg                                                         >  5:105055/1‑135 (MQ=255)                                                                       caTTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATg                                                         >  5:351560/1‑135 (MQ=255)                                                                       caTTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATg                                                         >  5:75220/1‑135 (MQ=255)                                                                                       gcagCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTggcgg                                                                                          >  7:171534/1‑86 (MQ=255)                                                                                       gcagCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTggcgg                                                                                          <  8:171534/86‑1 (MQ=255)                                                                                         agCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGa                                       <  4:198088/135‑1 (MQ=255)                                                                                         agCGCTCGATCGCAGCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTACAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGa                                       <  6:206329/135‑1 (MQ=255)                                                                                          gcgcTCGATCGCATCCAGTGGCTTAGCGACGTTGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGGCAGCGCGCTGAGCCAATGGCTTGCCCTGCTGCTGGAc                                      <  2:368759/135‑1 (MQ=255)                                                                                            gcTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGa                                    <  7:172171/135‑1 (MQ=255)                                                                                            gcTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGa                                    <  2:381618/135‑1 (MQ=255)                                                                                            gcTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGa                                    <  2:136734/135‑1 (MQ=255)                                                                                            gcTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGa                                    <  1:325523/135‑1 (MQ=255)                                                                                              tCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAg                                                               <  2:368821/106‑1 (MQ=255)                                                                                              tCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAg                                                               >  1:368821/1‑106 (MQ=255)                                                                                              tCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAg                                  <  3:94290/135‑1 (MQ=255)                                                                                                 aTCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAGGc                                <  5:229812/134‑1 (MQ=255)                                                                                                     cATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAGGCaagga                           <  4:364043/135‑1 (MQ=255)                                                                                                        ccAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCggcca                                                                                                      >  1:240308/1‑57 (MQ=255)                                                                                                        ccAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCggcca                                                                                                      <  2:240308/57‑1 (MQ=255)                                                                                                        ccAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAGGCaaggaagg                        >  2:89652/1‑135 (MQ=255)                                                                                                          aGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTATAGCGCGGCCAGGCAAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAGGCAAGGAAGGGc                      <  7:221424/135‑1 (MQ=255)                                                                                                               cGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCggcca                                                                                                      >  3:263403/1‑50 (MQ=255)                                                                                                               cGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCggcca                                                                                                      <  4:263403/50‑1 (MQ=255)                                                                                                                gTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGTCTTGCCCTGCTGCTGGACGAAGGCAAGGAAGGGCAGGcc                 <  1:2145/134‑1 (MQ=255)                                                                                                                         aTGCCGTGCAACGCCTGGCATGCCGGGTAAAGCGCGGCCAGGCCAGTGGAGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAGGCAAGGAAGGGCAGGCCGCGCAGTtgc       <  8:24733/135‑1 (MQ=255)                                                                                                                              gTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAGGCAAGGAAGGGCAGGCCGCGCAGTTGCTGCCa  <  5:42123/135‑1 (MQ=255)                                                                                                                                  aaCGTCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAg                                  >  4:38416/1‑99 (MQ=255)                                                                                                                                  aaCGTCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAg                                  >  4:33598/1‑99 (MQ=255)                                                                                                                                  aaCGTCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAg                                  <  3:33598/99‑1 (MQ=255)                                                                                                                                  aaCGTCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAg                                  <  3:38416/99‑1 (MQ=255)                                                                                                                                      |                                                                                                                               GCCTGGGCCAGACCTATGGCGGCGAGCAGCCGGCGGCGGCACCGGAGTTCCATCCGCTGCTGCCGTTGCACATTGCGTTGCAGCAGGCAGCGCTCGATCGCATCCAGTTGCGTAGCGACGATGCCGTGCAACGCCTGGCATGCCTGGTAAAGCGCGGCCAGGCCAGTGGCGGCATGATGCTTACCGTCAGCGCCCTGAGCCAATGGCTTGCCCTGCTGCTGGACGAAGGCAAGGAAGGGCAGGCCGCGCAGTTGCTGCCA  >  ACB122/879235‑879494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑