Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,052,536 T→G 24.3% S90R (AGC→CGC)  argO ← Arginine exporter protein ArgO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,052,5360TG24.3% 59.6 / 2.9 55S90R (AGC→CGC) argOArginine exporter protein ArgO
Reads supporting (aligned to +/- strand):  ref base T (21/19);  new base G (6/9);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGGTGTCGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGATGGCGTCACAG  >  ACB122/1052415‑1052669
                                                                                                                         |                                                                                                                                     
ccGGTGTCGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTg                                                                                                                          <  7:180102/134‑1 (MQ=255)
 cGGTGGCGAGGTACACGGGCGGGTTCCGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGGGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTgg                                                                                                                         <  7:316815/135‑1 (MQ=255)
       cGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTCGCGCCGt                                                                                                                   >  2:196370/1‑135 (MQ=255)
       cGAGGTACACGTGCGGGTTCAGCAGGGGCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGt                                                                                                                   <  2:200023/135‑1 (MQ=255)
       cGAGGTACACGGGCGGGTTCCGCAGGGCCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGGGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGt                                                                                                                   <  1:182716/135‑1 (MQ=255)
         aGGTACACGGGCGGGTGCAGCAGGGGCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCGCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTAc                                                                                                                 <  1:42741/135‑1 (MQ=255)
              cacGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCATGGCGCCGTACCAGa                                                                                                             >  8:293631/1‑134 (MQ=255)
              cacGGGCGGGTTCAGCAGGGCCCCCGCCCGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCGGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGAt                                                                                                            <  1:136707/135‑1 (MQ=255)
                 gTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGGGCGGCGCCGCGCCGGGGCGCCGGACCCGCTCa                                                                                                          >  1:40934/1‑134 (MQ=255)
                 gTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCCCGCCTGGGCGCCGTACCAGGTCa                                                                                                          >  7:92309/1‑134 (MQ=255)
                        ttCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCGTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACt                                                                                                  <  8:278114/135‑1 (MQ=255)
                         tCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGGCGGACCAGAACAGGAAAACTg                                                                                                 >  2:264231/1‑135 (MQ=255)
                            gcagGGTCACCGCCAGGGCGCTGAGCAATACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTgcgc                                                                                              >  7:79341/1‑135 (MQ=255)
                              agGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGGTGCGCAGCGCCTTGGGGCCGTCGCAGCTCAGGAGCACTgcgcag                                                                                            >  7:239277/1‑133 (MQ=255)
                                gggccccGCCCGGGCGCGGAGCCGTACGGCGCGACGTGAGCGACCGCCTTGGCCTTGCTGGGGCGGCCGGCTCGGCTGGGCGCAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCgccgcc                                                                                          <  5:348215/130‑1 (MQ=255)
                                ggTCACCGCCAGGGCGCGGAGCCGTACGGCGCGACGGGAGCGAACGCCGTGGCCTTGCTGGTGCTGCAGGCGCTGCTGGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCgccgcc                                                                                          <  7:252397/135‑1 (MQ=255)
                                   cACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCGTGGCGCCGTACCAGCTCAGGAACACGGCGCCGccccc                                                                                       >  7:232061/1‑134 (MQ=255)
                                       gCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGACCGCCTGGGCCTTGCTGGTGCGGCAGGCTCTGCTTGGAGCAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCgg                                                                                   <  8:232345/135‑1 (MQ=255)
                                               gcTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCCGCGTACCAGATCATGGACACAACGCCGCCCCAGCGGGCAATCGc                                                                           >  7:195117/1‑135 (MQ=255)
                                               gcTGAGCAGTACGGCGCGACGGTCGCGAACGCGTTGGCCTTGGGGGGGGTGCTGGCTCTGCTTGGCGCTGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGc                                                                           <  7:158965/135‑1 (MQ=255)
                                                 tGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCa                                                                         <  4:361356/135‑1 (MQ=255)
                                                 tGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTGGCTGGTGCGGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCa                                                                         <  5:247220/135‑1 (MQ=255)
                                                   aGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCCCGCCTTGGCGCCGTACCAGATCAGGCACCCAGCGCCGCCCCAGCGGGGACTCGCCAg                                                                        >  6:57487/1‑134 (MQ=255)
                                                     ccgTACGGCGCGACGTGAGCTAACGCCTGGGCCTTGCTGGGGCGGCTGGCTCTGCTTGGAGCTGGCGCTGCGCAGGGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCaa                                                                     <  6:280333/133‑1 (MQ=255)
                                                       gTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGGGCTGCAGGCTCGGCTGGGAGCAGGCGCTGCGCAGCGCCTTGGCGCAGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAgg                                                                   <  1:91995/135‑1 (MQ=255)
                                                           ggCGCGACGTGAGCGACCGCGTGGGCCTTGCTGGGGCTGCAGGCTCTGCTTGGAGCAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCgg                                                               <  3:158096/135‑1 (MQ=255)
                                                           ggCGCGACGTGAGCGAACGCCTTGGCCTTGCGGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCgg                                                               <  8:132666/135‑1 (MQ=255)
                                                             cgcgACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCTTGGCGCCGGTCCAGATCAGGAACAAAGCGCCGCCCCCGCGGGCCATCGGCAGCCAGGGCgggg                                                             >  3:159675/1‑134 (MQ=255)
                                                                gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGGGCGGCGCCGCGCCCTGGCGCGGGGCCAGAACAGGCACCCAGCGCCCCCCCCCCGGGCCCTCGCCACCCACGGCGggtggt                                                          >  5:280333/1‑135 (MQ=255)
                                                                gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGGGGCGCAGCGCCTTGGCGGCGGACCAGCGCAGGTACACTGCGCCGCCCCCGCGGGCCATGGGCAGCAAGGTCGGGTtgt                                                          >  7:303080/1‑135 (MQ=255)
                                                                gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCGTGGCGCCGGGCCAGATCAGGAACCCAGCGCCGCCCCCGCGGGCCATCGCCAGCAAGGGCGGGgtgt                                                          >  5:273006/1‑135 (MQ=255)
                                                                gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCCGCGGGCACTCGCCAGCAAGGTCGGGTtgt                                                          >  6:361058/1‑135 (MQ=255)
                                                                gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTGGGCGCCGGCCCAGATCAGGCACACTGCGCCGCACCAGCGGGCCATCGCCACCAAGGTCGGGgtgt                                                          >  7:47450/1‑135 (MQ=255)
                                                                gACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCGGCGCCGCGCCGGGGCGCCGGCCCAGCACGGGCCCCCAGCGCCCCCCCCGCGGGCCCGCGCCAGCCcgggcggggggt                                                          >  5:163112/1‑131 (MQ=255)
                                                                     gAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGGGCGGGGCCGCGCCGTGGCGCCGGGCCAGCTCAGGCACCCTGCGCCGCCCCCGCGGGCCCTCGGCCGCAAGGGCGGGTtgtgtggg                                                     >  1:211729/1‑133 (MQ=255)
                                                                               cttggccttgCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTTCCAGATCAGGAACCCTGCGCCGCCCCCGCGGGCAATCGCCAGCCAGGGCGGGTTGTGTGCCAGCACGGTgg                                           >  2:96276/1‑135 (MQ=255)
                                                                               cttggccttgCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCCGCACGGTgg                                           >  4:75264/1‑135 (MQ=255)
                                                                               cttggccttgCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCATGGCGCCGGCCCAGATCAGGAACACTGCGCCGCCCCCCCGGGCCAACGCCAGCCACGGCGGGTTGTGTGGCCGCACGgggg                                           >  1:303544/1‑135 (MQ=255)
                                                                                ttggccgtgCTGGTGCTCCAGGCTCTGCTTGGCGCAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTg                                            <  1:264231/133‑1 (MQ=255)
                                                                                   gccttgCTGGGGCGGCCGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGcc                                         <  4:307283/133‑1 (MQ=255)
                                                                                      ttgCTGGTGCGGCAGGCTCGGCTGGGAGCAGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTcc                                    <  3:122146/135‑1 (MQ=255)
                                                                                            gTGCTACAGGCTCTGCTTGGAGCAGGCGCTGCGCCGCGCCTTGGCGCCGGACCCGATCAGGAACACAGCGCCCCCCCCCCGGGCCATCGCCCGCCAGGTCGGGTTGTGTGGCAGCACGGTGGCCCGGCCGAATAc                              >  8:24338/1‑135 (MQ=255)
                                                                                                tgcAGGCTCGGCTGGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCcg                          <  2:328520/135‑1 (MQ=255)
                                                                                                ggccggCTGTGCGGGGCGCCGGCGCGGCGCCGCCCCTTGGCGCCGAACCAGAGCAGGAACCCTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCcg                          <  1:190116/131‑1 (MQ=255)
                                                                                                 gcAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGGACCAGATCAGGGACCCTGCGCCGCCCCCGCGGGCACTCGCCAGCAAGGTCGGGTTGTGTGCCAGCCCGGTGGCCCGTCCGAATACTCcgg                         >  3:115289/1‑135 (MQ=255)
                                                                                                 gcAGGCTCTGCTTGGAGCAGGCGCGGCGCCGCGCCTTGGCGCCGTACCAGATCAGGACCACTGCGCCGCCCCCGCGGGGCATCGCCCGCAAGGTCGGGGTGTGTGGCAGCCCGGTGGCCAGGCCCGATACTCcgg                         >  7:73412/1‑135 (MQ=255)
                                                                                                      ctctGCTGGGAGCCGGCGCGGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGcc                    <  4:358253/135‑1 (MQ=255)
                                                                                                          gCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCCGTCCGCATACTCCGGCGGCCACGa                >  6:73750/1‑135 (MQ=255)
                                                                                                          gCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGGACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGa                >  8:366013/1‑135 (MQ=255)
                                                                                                            ttGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGa                <  6:159718/133‑1 (MQ=255)
                                                                                                            gtGGCGCCGGCGCGGCGCCGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAgg              <  1:196370/134‑1 (MQ=255)
                                                                                                                 gCAGGCGCTGCGATGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGTGCAATCGCCAGCAAGGTCGGGTTGTGTGACTGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGTATGGc         <  6:313577/135‑1 (MQ=255)
                                                                                                                   aGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGATGGCGt       <  8:239277/135‑1 (MQ=255)
                                                                                                                      cgcTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGATGGCGTcac    <  7:293631/135‑1 (MQ=255)
                                                                                                                        cTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGACGGCGTCACAg  >  2:40076/1‑135 (MQ=255)
                                                                                                                         |                                                                                                                                     
ACGGTGTCGAGGTACACGTGCGGGTTCAGCAGGGTCACCGCCAGGGCGCTGAGCAGTACGGCGCGACGTGAGCGAACGCCTTGGCCTTGCTGGTGCTGCAGGCTCTGCTTGGAGCAGGCGCTGCGCAGCGCCTTGGCGCCGTACCAGATCAGGAACACTGCGCCGCCCCAGCGGGCAATCGCCAGCAAGGTCGGGTTGTGTGCCAGCACGGTGGCCAGTCCGAATACTCCGGCGGCCACGAGGATGGCGTCACAG  >  ACB122/1052415‑1052669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑