Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,062,909 T→G 17.3% F341V (TTT→GTT)  aroP_1 → Aromatic amino acid transport protein AroP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,062,9090TG17.3% 155.2 / 3.2 76F341V (TTT→GTT) aroP_1Aromatic amino acid transport protein AroP
Reads supporting (aligned to +/- strand):  ref base T (27/33);  new base G (12/2);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 7.25e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTACCCACCTCAAATTCCG  >  ACB122/1062778‑1063039
                                                                                                                                   |                                                                                                                                    
cGGTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTg                                                                                                                                   <  6:171314/135‑1 (MQ=255)
cGGTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTg                                                                                                                                   <  5:20252/135‑1 (MQ=255)
 ggTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGc                                                                                                                                  <  1:137968/135‑1 (MQ=255)
 ggTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGc                                                                                                                                  <  6:11743/135‑1 (MQ=255)
 ggTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGc                                                                                                                                  <  6:191099/135‑1 (MQ=255)
 ggTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGc                                                                                                                                  <  4:165361/135‑1 (MQ=255)
 ggTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGc                                                                                                                                  <  3:35531/135‑1 (MQ=255)
 ggTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGc                                                                                                                                  <  3:125064/135‑1 (MQ=255)
 ggTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGGTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGc                                                                                                                                  <  4:354023/135‑1 (MQ=255)
  gtgtACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGc                                                                                                                                  <  1:240228/134‑1 (MQ=255)
      acaacaGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCg                                                                                                                             <  8:1373/135‑1 (MQ=255)
                tgtgtACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTgtgcgt                                                                                                                  >  1:294675/1‑135 (MQ=255)
                tgtgtACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTgtgcgt                                                                                                                  >  6:74016/1‑135 (MQ=255)
                tgtgtACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTgtgcgt                                                                                                                  >  7:366838/1‑135 (MQ=255)
                tgtgtACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTgtgcgt                                                                                                                  >  7:228365/1‑135 (MQ=255)
                tgtgtACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGAACGGCCTgggcgt                                                                                                                  >  8:36013/1‑135 (MQ=255)
                  tgtACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTgtgcgtgc                                                                                                                >  7:218499/1‑135 (MQ=255)
                     aCGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCAGTTGCGACCGGCCTGTGCGTGCTgc                                                                                                             >  2:307448/1‑134 (MQ=255)
                       gCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGAt                                                                                                            >  5:314127/1‑134 (MQ=255)
                               ccGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTAcc                                                                                                    >  6:369007/1‑134 (MQ=255)
                               ccGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTAcc                                                                                                    >  2:28372/1‑134 (MQ=255)
                               ccGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTAcc                                                                                                    >  7:239973/1‑134 (MQ=255)
                               ccGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCGTTGGGGCCGGGCGGTGCCTGCGGATCAAATa                                                                                                      >  5:315105/1‑132 (MQ=255)
                               ccGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCGTTGCGGCCGGCCGGTGGGTGCTGATCAATTaacg                                                                                                   >  1:181743/1‑132 (MQ=255)
                               ccGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCCTTGCGCCCCGCCTGTGCGGGCTGCTCAATTATCt                                                                                                   >  3:27451/1‑135 (MQ=255)
                                    tGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGCGCCGTAGCGGCGTGCCGCAGACCGCCCTGGCCGTGTCGGCCTTAGCGACCGGCCTGGGCCGTCTGATGAAATACCAGATgg                                                                                             >  1:25179/1‑134 (MQ=255)
                                    tGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGc                                                                                             >  6:347637/1‑135 (MQ=255)
                                    tGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCGTTGCGACCGGCCGGTGCGTGCGGATCAATTAACTGATGc                                                                                             >  3:335272/1‑135 (MQ=255)
                                      cTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCcg                                                                                           >  3:364370/1‑135 (MQ=255)
                                        gTTTGGGCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCcggg                                                                                         <  5:89170/135‑1 (MQ=255)
                                          ttGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCcggg                                                                                         <  5:70682/133‑1 (MQ=255)
                                            ggcctggccAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGGCCGGCCTGGGCGGGCGGATCAATTTCCTGATGGcgggccgg                                                                                     >  1:377891/1‑135 (MQ=255)
                                            ggcctggccAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCcgggcggg                                                                                     >  4:91740/1‑135 (MQ=255)
                                            ggcctggccAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCcgggcgg                                                                                      <  8:366838/134‑1 (MQ=255)
                                            ggcctggccAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTAGGGCGTTGCGGCCGGCCCGGTGGTGCGTCGAAATTTCCTGATGCcgggcggg                                                                                     >  1:186422/1‑135 (MQ=255)
                                            ggcctggccAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGGCGGCCGTTGGGCCCGGCCGGGGCGGGGGGATCAATTACCTGATGCcgggcggg                                                                                     >  6:224151/1‑135 (MQ=255)
                                            ggcctggccAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCCTGTCGGCCGTTTCGCCCGGCCGGTTGCGTCTGATCAAATTCCTGAAGCccggcggg                                                                                     >  2:354321/1‑135 (MQ=255)
                                              cctggccAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCgggg                                                                                    <  3:175364/134‑1 (MQ=255)
                                                   ccagccagGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTg                                                                              <  5:74016/135‑1 (MQ=255)
                                                    cagccagGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGCCCGCGCTGGCCGTGTCGGGCGTTGCGCCCGGCCTGTGCGTGCGGGGCAATTACCGGCTGCCCGGCGGGGCCGTTgg                                                                             >  2:137067/1‑135 (MQ=255)
                                                    cagccagGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGCCCGCGCTGGCCGTGGCGGCCGTTTGGGCCGGCCGGTGGGGGCGGGGGACTTTCCTGATGCCGGGGGGGGGCGTTgg                                                                             >  1:320817/1‑135 (MQ=255)
                                                    cagccagGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCAGGCCGTGTCGGCCGTTGCGCCCGGCCTGTGCGTGCGGATCAATTACCGGATTCCGGGCGGGGCCTTTgg                                                                             >  3:264703/1‑135 (MQ=255)
                                                          gggCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCt                                                                       <  1:126085/135‑1 (MQ=255)
                                                          gggCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCt                                                                       <  3:304277/135‑1 (MQ=255)
                                                          gggCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCt                                                                       <  2:172156/135‑1 (MQ=255)
                                                          gggCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCGTTTCGCCCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCGTTGGTTTggt                                                                       >  5:101843/1‑133 (MQ=255)
                                                               aTGCGCCGCGTCAGTTGGTGAAGGTGAGCCGTAGCGGCGTGCCGCAGACCGCGCAGGCCGTGTTCGCCCTTGCGCCCGCGCTGTGCGTGGGGCGCAATTACCTGATTCCCGGGGGGGGCGGGTGGTTTggggtgg                                                                   >  2:371052/1‑129 (MQ=255)
                                                                   gccgcGTCAGTTGCGGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCGTTGCGACCGGCCTGGGCGTGCTGATCAATTACCTGGTTCCGGGCGGGGCCTTTGGTTTGCTGTTGGCGtg                                                              >  2:259621/1‑134 (MQ=255)
                                                                    ccgcGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTg                                                             <  2:294675/135‑1 (MQ=255)
                                                                    ccgcGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGCGTCGGCCTTTGCGACCGGCCTGGGCGTGCTGCTCCATTACCTGAGGCCGGGCGGGGCCGTGGGTTTGCGGCTGGGGTTg                                                             >  7:176401/1‑135 (MQ=255)
                                                                     cgcgTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGCCCGGCCGGTGGGTGCTGCTCAATTTCCTGATGCCGGGCGGGGCCGTTGGTTTGCGGATGGCGGTgg                                                            >  1:96570/1‑135 (MQ=255)
                                                                        gTCAGTTGCTGAAGGTGAGCCGTAGCGGCGGGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTTCCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAg                                                         >  5:150016/1‑135 (MQ=255)
                                                                              tGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCcg                                                   <  8:66576/135‑1 (MQ=255)
                                                                              tGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCcg                                                   <  2:25179/135‑1 (MQ=255)
                                                                                                  ggcgTGCCGCTGACCGCGCTGGCCGTGTCGGGCTTTGGGGCCGGCCGGGGCGGGGGGGTCAATTTCCGGCTGCCGGGCGGGGGCGTTGGTTTGGGGATGGCGGTGGGCGTGTGCGCGGTGGGGGTCGACTggggg                               >  6:36275/1‑133 (MQ=255)
                                                                                                    cgggCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAg                             <  7:242366/132‑1 (MQ=255)
                                                                                                    cgggCCGCTGACCGCGCTGGCCGTGTCGGCCGTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAg                             <  5:36275/132‑1 (MQ=255)
                                                                                                    cgTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAg                             <  1:309142/135‑1 (MQ=255)
                                                                                                    cgTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAg                             <  5:224151/135‑1 (MQ=255)
                                                                                                    cgTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAg                             <  4:227608/135‑1 (MQ=255)
                                                                                                    cgTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAg                             <  1:5873/135‑1 (MQ=255)
                                                                                                         cGCTGACCGCGCTGGCCGTGTCGGGCTTTGGGACCGGCCGGGGCGTGCTGATCAATTACCTGCTGCCGGGCGGGGCCTTTGGTTTGCTGATGGGGTTGGCAGTGTCCGCGGTGGTGATCAACTGGGCGCGCATCa                        >  1:37945/1‑135 (MQ=255)
                                                                                                         cGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGCTGAACTGGGCGAGCCTCa                        >  3:159104/1‑135 (MQ=255)
                                                                                                         cGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCa                        <  6:18711/135‑1 (MQ=255)
                                                                                                         cGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGGGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCa                        <  5:132281/135‑1 (MQ=255)
                                                                                                               ccGCGCTGGCCGTGTCGGCCTGTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGACGGGCGGGGCGGTTGGTTTGCGGATCGCGGTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTa                  <  5:291063/135‑1 (MQ=255)
                                                                                                               ccGCGCTGGCCGGGTCGGCCGTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTa                  <  7:364554/135‑1 (MQ=255)
                                                                                                                 gcgcTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGt                                                               >  7:372270/1‑88 (MQ=255)
                                                                                                                 gcgcTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGt                                                               <  8:372270/88‑1 (MQ=255)
                                                                                                                   gcTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGGTTGCTGATGGCGTTGGCTGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTACCCa              >  1:1661/1‑135 (MQ=255)
                                                                                                                     tGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTACCCAcc            >  1:147363/1‑135 (MQ=255)
                                                                                                                      ggCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCCGCATTACCCAcc            >  1:248883/1‑134 (MQ=255)
                                                                                                                      ggCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGGTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTACCCAcc            >  8:248314/1‑134 (MQ=255)
                                                                                                                        ccGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTACCCACCt           <  8:239973/133‑1 (MQ=255)
                                                                                                                        ccGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTACCCACCTc          <  4:364370/134‑1 (MQ=255)
                                                                                                                                gCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTACCCACCTCAAATTCCg  >  3:45173/1‑134 (MQ=255)
                                                                                                                                   |                                                                                                                                    
CGGTGTACAACAGCTGTGTGTACGCCAATAGCCGTATGCTGTTTGGCCTGGCCAGCCAGGGCGATGCGCCGCGTCAGTTGCTGAAGGTGAGCCGTAGCGGCGTGCCGCTGACCGCGCTGGCCGTGTCGGCCTTTGCGACCGGCCTGTGCGTGCTGATCAATTACCTGATGCCGGGCGGGGCCTTTGGTTTGCTGATGGCGTTGGCAGTGTCCGCGTTGGTGATCAACTGGGCGAGCATCAGCATTACCCACCTCAAATTCCG  >  ACB122/1062778‑1063039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: