Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,084,026	T→G	6.3%	V143G (GTA→GGA)	CJ019_01018 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,084,026	0	T	G	6.3%	190.7 / 4.6	64	V143G (GTA→GGA)	CJ019_01018	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/39);  new base G (2/2);  total (23/41)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

AGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAATGGAAATCCACCACACCAAGCAT  >  ACB122/1083892‑1084154                                                                                                                                       |                                                                                                                                 aGCCTTGCGCCACAGTGCGCGCAATTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGt                                                                                                                                  <  8:193644/135‑1 (MQ=255)    cTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAac                                                                                                                               >  6:34458/1‑135 (MQ=255)                     gcACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCCGCGCGCTCGCGGCCCAGCCGGCTGTTACACCTAGAACCCAGGccg                                                                                                              >  1:192592/1‑134 (MQ=255)                     gcACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCt                                                                                                              >  6:118407/1‑135 (MQ=255)                            cacCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGCACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGa                                                                                                         <  4:351326/133‑1 (MQ=255)                            cacCGAGCTGCTGAGGTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAAt                                                                                                       <  5:36150/135‑1 (MQ=255)                             acCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAAt                                                                                                       >  7:286917/1‑134 (MQ=255)                             acCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAAt                                                                                                       <  2:249279/134‑1 (MQ=255)                                 aGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACt                                                                                                  <  5:367629/135‑1 (MQ=255)                                 aGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACt                                                                                                  <  5:153945/135‑1 (MQ=255)                                 aGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACt                                                                                                  <  5:109817/135‑1 (MQ=255)                                 aGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACt                                                                                                  <  8:162076/135‑1 (MQ=255)                                 aGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACt                                                                                                  <  3:370913/135‑1 (MQ=255)                                 aGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCAGCGTACCGAGCGCTTGATCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACt                                                                                                  <  1:107876/135‑1 (MQ=255)                                 aGCTGCGGAGATGCATTGCCGCGTAAGGCCAGACCCCGCGTCGCGAGCGCTTGCTCAACCGCCACAGCGGCGGGGCCAGCGAGCTCGCGGCCCAGCCGGATGTAACACCTAGAACCCATGCCTACGGAAATCACt                                                                                                  <  2:211441/135‑1 (MQ=255)                                  gctgctGAGTTTGTTTGCCGAGTATGGCCTGTTCCCGCGTTGCGTGCGCTTGCTCATCCGCCACAGCGGCGGGGCCAGCGAGCTCGCTGGCTAGGCGGATGTAACACCTAGAACCCATGCGTACGGAATTCACTg                                                                                                 <  5:343444/135‑1 (MQ=255)                                  gctgctGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 <  7:32899/135‑1 (MQ=255)                                  gctgctGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 <  4:153566/135‑1 (MQ=255)                                  gctgctGAGTTTCATTGCAGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCGACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 <  4:353428/135‑1 (MQ=255)                                   ctgctgAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGa                                                                                                <  2:159609/135‑1 (MQ=255)                                    tgctgAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGCGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGAc                                                                                               >  6:61058/1‑135 (MQ=255)                                    tgctgAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCACGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGAc                                                                                               >  2:224875/1‑135 (MQ=255)                                    tgctgAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGa                                                                                                >  8:102541/1‑134 (MQ=255)                                    tgctgAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGa                                                                                                >  3:128666/1‑134 (MQ=255)                                    tgctgAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGAc                                                                                               >  6:68464/1‑135 (MQ=255)                                    tgctgAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGAc                                                                                               >  4:114308/1‑135 (MQ=255)                                       tgAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACt                                                                                                  <  8:286917/129‑1 (MQ=255)                                         aGTTTCATTGCCGAGTATGGCCAATACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGcc                                                                                          <  4:207775/135‑1 (MQ=255)                                              cATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGCGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 >  4:214839/1‑123 (MQ=255)                                              cATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACCCCTAGAACCCATGCCTACGGAATTCACTGACAGGCCCCaga                                                                                     >  6:346198/1‑133 (MQ=255)                                              cATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 <  3:214839/123‑1 (MQ=255)                                                   ccGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTg                                                                                >  8:275729/1‑135 (MQ=255)                                                      aGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCt                                                                             <  1:311374/135‑1 (MQ=255)                                                      aGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCt                                                                             <  8:336191/135‑1 (MQ=255)                                                             ccAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTg                                                                       <  4:44592/134‑1 (MQ=255)                                                                    ccGCGTACCGAGCGCTTGCTCACCCGCCACAACGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTCACACCTAGCACCCATGCCTACGGAATTCACTGACAAGCCCCAAACCTTGCCTGCATTGCCCTACGc                                                               >  7:127997/1‑135 (MQ=255)                                                                      gcgTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCt                                                             <  6:44696/135‑1 (MQ=255)                                                                       cgTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTa                                                            <  4:287623/135‑1 (MQ=255)                                                                       cgTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGACCCATACCTTGCCTGCATTGCCATACGCCTa                                                            <  7:313668/135‑1 (MQ=255)                                                                         tACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCCGCGAGCTCGCTGCCCAGCCGGATGTAACCCCTAGAACCCCTGCCTACGGAATTCACTGACATGCCCCCTACCTTGCCTGCCTTGCCATACGCCTACg                                                          >  3:374232/1‑135 (MQ=255)                                                                         tACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGCGCTCGCTGCCAAGCCGGATGTAACACCTAGCACCCATGCCTAAGGAATTCACTGCCCTGCCCCCTACCGTGCCTGCATTGCcctacgcctacg                                                          >  8:55687/1‑135 (MQ=255)                                                                         tACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGcc                                                                     <  1:323880/124‑1 (MQ=255)                                                                         tACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGcc                                                                     >  2:323880/1‑124 (MQ=255)                                                                          aCCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGa                                                         <  5:118407/135‑1 (MQ=255)                                                                          aCCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGGAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 >  8:370775/1‑95 (MQ=255)                                                                          aCCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGGAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 >  8:365690/1‑95 (MQ=255)                                                                          aCCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGGAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 <  7:370775/95‑1 (MQ=255)                                                                          aCCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGGAACACCTAGAACCCATGCCTACGGAATTCACTg                                                                                                 <  7:365690/95‑1 (MQ=255)                                                                            cGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATg                                                       <  3:285636/135‑1 (MQ=255)                                                                                cgattGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCt                                                   <  4:235306/132‑1 (MQ=255)                                                                                     gCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTAACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGaa                                               >  4:181857/1‑134 (MQ=255)                                                                                       tCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGACTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGTAcc                                             <  6:124488/134‑1 (MQ=255)                                                                                          aCCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACcacac                                         <  3:114308/135‑1 (MQ=255)                                                                                                acaGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGAt                                   <  5:170011/135‑1 (MQ=255)                                                                                                     gcCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCa                              <  1:166829/135‑1 (MQ=255)                                                                                                            ccAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCCTGCCTACGGAATTCCCTGACAGGCCCCCGACCGTGCCTGCCTTGCCATACGCCTACGATGCGCTGGCACCCCACATTGCTGCCCTGACCATg                       >  2:334752/1‑135 (MQ=255)                                                                                                            ccAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATAGCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAATg                       <  5:34458/135‑1 (MQ=255)                                                                                                            ccAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCt                                                                             >  2:247749/1‑81 (MQ=255)                                                                                                            ccAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCt                                                                             <  1:247749/81‑1 (MQ=255)                                                                                                            ccAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAAt                        >  8:294758/1‑134 (MQ=255)                                                                                                                        cTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAATGGAAATccacca            <  4:128666/134‑1 (MQ=255)                                                                                                                        cTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAATGGAAATccacca            <  4:360296/134‑1 (MQ=255)                                                                                                                            cAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAATGGAAATCcaccacacc        <  3:69898/134‑1 (MQ=255)                                                                                                                              aGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAATGGAAATCCACCACACCAAg     <  7:294758/135‑1 (MQ=255)                                                                                                                                 cGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAATGGAAATCCACCACACCAAGCAt  <  5:172573/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 AGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACCTAGAACCCATGCCTACGGAATTCACTGACATGCCCCATACCTTGCCTGCATTGCCATACGCCTACGATGCGCTGGAACCACACATTGATGCCCAGACAATGGAAATCCACCACACCAAGCAT  >  ACB122/1083892‑1084154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑