| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,246,491 | T→G | 39.0% | intergenic (‑84/+23) | rnfA ← / ← metG | Na( )‑translocating ferredoxin:NAD( )oxidoreductase complex subunit A/Methionine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,246,491 | 0 | T | G | 39.0% | 14.6 / 5.3 | 41 | intergenic (‑84/+23) | rnfA/metG | Na( )‑translocating ferredoxin:NAD( )oxidoreductase complex subunit A/Methionine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (8/15); new base G (13/3); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.17e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAG > ACB122/1246362‑1246619 | gattgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGTGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATT‑TTTGTGCGCGGCAGGCC‑GCGcccc > 6:14166/1‑133 (MQ=255)gattgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCGCCca > 6:81384/1‑134 (MQ=255) tCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCGACCCCCATGGc > 7:259672/1‑135 (MQ=255) gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGCGCG‑GGCAGGGC‑GCGCCCACAATTGCCCg > 7:369694/1‑135 (MQ=255) gcggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATGGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGGGGGAGCG‑GGCAGGCC‑GCGCCCACAATGGCCCGCa < 4:83505/135‑1 (MQ=255) gcggcgCTGAGCAGGACCAGGATGTAGGCATTCATGAGATGCCGATAGCCGGGAAGCGGGGCG‑ATTGGCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCa < 2:242428/135‑1 (MQ=255) ggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGTGCG‑GGCCGGCC‑GCTGCCACCATGGCCCGCCgg > 7:221308/1‑135 (MQ=255) ggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGTGCG‑GGCCGGCC‑GCGGCCCCAAAGGCCCGCAgg > 2:335405/1‑135 (MQ=255) ggcgCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGCGCG‑GGCCGGCC‑GCGCCCCCAATGGCCCGCAgg > 7:215764/1‑135 (MQ=255) tGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGTGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCggg > 4:343343/1‑134 (MQ=255) tGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCCGGGC‑GCGACCACAATGGCCCGCAGGGCggg > 6:116764/1‑134 (MQ=255) aGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGGGCG‑GGCCGGCC‑GCGCCCCCCAAGGCCCCCAGGGCCGTTAGtt > 7:93/1‑135 (MQ=255) aGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACt > 4:188431/1‑134 (MQ=255) aGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTGGACATGAAGGGGCCTGCGTAATTGTGGGTGCG‑GGCAGGGC‑GCTCCCGCAATGGCCCGCAGGGCgggttggt > 3:132245/1‑129 (MQ=255) aCCAGTATGTAGTCATTCATGATATGACGATCGCCGGGAAGCGGGGCG‑ATGGTCCGGGTTTGACATGATGGGGCCTGCGTAATTGGGGGCGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGAt < 1:80090/135‑1 (MQ=255) aCCAGTATGTAGTCATTCATGAGATGACGATAGCCGGGAAGCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGAt < 5:179884/135‑1 (MQ=255) tGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTTGGGGCC‑GGGACGCC‑GCACCCCCCAAAGCCCCCACGGGGGGTACATTATGCGctgg > 1:78690/1‑135 (MQ=255) tcattcatGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGGGCG‑GGGAGGCC‑GCGGCCACAATGGGCCGCAGGGCGGGTACTTGATTCgctgggctgg > 3:145316/1‑135 (MQ=255) tcattcatGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCCCAAATGCCCCCAGGGCGGGTACTTGATTCGctggcctgg > 6:68056/1‑135 (MQ=255) atGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGGGCG‑GGGAGGGC‑GCGCCCCCAAAGGCCCGCACGGGGGGTTCTTTATTCGCTGGGCAGGCTTGgc > 7:30727/1‑135 (MQ=255) atGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCCGGCC‑GCGCCCACAATTGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgc > 3:148366/1‑135 (MQ=255) gagaTGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGCGCG‑GGCCGGCC‑GCTCCCACAATGGCCCGCCGGGGGGGTACTTGATGCGCTGGCCTGGCTTTgcgc > 1:243680/1‑135 (MQ=255) agaTGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGGGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgcgc < 1:309729/134‑1 (MQ=255) aCGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCGCCCACCATGGCCCGCAGGGCGGTTTATTGATGCGCTGGGCAGGCGTTGcgccgcggg > 6:283669/1‑132 (MQ=255) aTAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCg > 4:44003/1‑135 (MQ=255) cgcGGGTATCCGGGCGAATGGCCCGGGTTTCACAGCAGGGGGCCTG‑GTTAATGGGGGTCCG‑GGCACGCCGGCCCCAAAAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCg < 8:221308/131‑1 (MQ=255) cggtactCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGGGGGCGCG‑GGCAGGCC‑GCGCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTc < 4:12528/129‑1 (MQ=255) gggAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTTGGGGCC‑GGCAGGCC‑GCGCCCCCAAAGGCCCCCACGGGGGTTACTTTATGCGCTGGCCTGGGTTTGCGCCGGGTTCCCGGCTcc > 6:229659/1‑134 (MQ=255) aTCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGCGCG‑GGCCGGCC‑GCTCCCCCCATGGCCCGCCGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGGCCGGGCTcacccg > 8:200985/1‑131 (MQ=255) ttGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAg < 8:369694/135‑1 (MQ=255) caggAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGc < 7:42817/131‑1 (MQ=255) cATGACGGGGCCTGCGTAATTGGGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGc < 3:343343/134‑1 (MQ=255) cATGACGGGGCCCGCGTAATTGTGGGCGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGc < 3:182412/134‑1 (MQ=255) cATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGc < 3:188431/134‑1 (MQ=255) cggggCCGGCGTAATGGGGGGAGCG‑GGCAGGCC‑GCGCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCa < 5:342916/133‑1 (MQ=255) aGGGGCCTGCGTAATTGGGGGCGCG‑GGCTGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCa < 8:267317/134‑1 (MQ=255) aGGGGCCTGCGTAATTGGGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCa < 6:348878/134‑1 (MQ=255) cggCGTATTTGGGGGCGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGa < 5:283669/133‑1 (MQ=255) cTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGa < 3:149442/135‑1 (MQ=255) ggCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAg < 2:42305/135‑1 (MQ=255) ggCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCAGCAGCCATGACCATGCCTTCGGACACGCCAAAg < 3:192570/135‑1 (MQ=255) | GATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATCGGGGCG‑ATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCG‑GGCAGGCC‑GCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAG > ACB122/1246362‑1246619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |