Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,308,712 A→C 37.6% T32P (ACG→CCG)  CJ019_01226 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,308,7120AC37.6% 36.5 / 8.0 52T32P (ACG→CCG) CJ019_01226hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (17/14);  new base C (18/3);  total (35/17)
Fisher's exact test for biased strand distribution p-value = 3.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGCCTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGAGCAGGAACGCTACGAG  >  ACB122/1308582‑1308846
                                                                                                                                  |                                                                                                                                       
cTGCGCCTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCcgccgcc                                                                                                                                     >  6:327805/1‑134 (MQ=255)
cTGCGCCTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGcc                                                                                                                                     >  2:335740/1‑134 (MQ=255)
cTGCGCCTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCa                                                                                                                                     >  4:238004/1‑135 (MQ=255)
 tGCGCCTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGccg                                                                                                                                    >  4:186509/1‑133 (MQ=255)
      cTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTCACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCAGCCTGc                                                                                                                               >  7:263111/1‑135 (MQ=255)
      cTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCCGCCGGc                                                                                                                               >  5:336214/1‑135 (MQ=255)
      cTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGc                                                                                                                               >  2:11649/1‑135 (MQ=255)
      cTGCAGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGc                                                                                                                               >  3:360229/1‑135 (MQ=255)
        gCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCCGCCGGCGt                                                                                                                             >  1:134663/1‑135 (MQ=255)
            aTACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGCCGTCAAGCCGGTGGTGCCGCCGCCGCCTGCGTCC‑gg                                                                                                                        >  5:270491/1‑135 (MQ=255)
                    ggCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCATGACGGTTTTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTg                                                                                                                <  8:306355/135‑1 (MQ=255)
                     gCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCAAACCCCCGACGTCAAGCCGGTGGTGCCGACCCAGCCTGCGTCC‑GGCGAAAGTg                                                                                                                >  2:66827/1‑134 (MQ=255)
                       aTAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCCGCCTGCGTCC‑GGCGAAAGTGCCa                                                                                                             >  2:46035/1‑135 (MQ=255)
                             tatcgaccATGCTGCGGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCGGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCTAAAGTGCAATTGAcc                                                                                                       <  2:255853/129‑1 (MQ=255)
                                 gTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCaa                                                                                                   >  7:292770/1‑135 (MQ=255)
                                  tCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGCCCTCAAg                                                                                                  >  6:91649/1‑135 (MQ=255)
                                   ccATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCCCAGCCGGCGTCC‑GGCGAAAGTGCCATTGACCGCaacc                                                                                                 >  5:265703/1‑133 (MQ=255)
                                          gccgctGATCATTCCGCTCCGTGCCGCCCCGTTAACCTCGCACCAGGACCCTGTCAAGCCACCCCCCGACGTCAGGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAgcg                                                                                          <  5:151656/135‑1 (MQ=255)
                                              ccgCTCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGGCGACGCAGCCAGCGGGC‑GGCGACAGTGCCATTGCCCTCGAGCGCCAgcgcccc                                                                                      >  1:214106/1‑132 (MQ=255)
                                              ccgATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGCCGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCCAGCGCCCGCGCGAc                                                                                      >  3:246185/1‑135 (MQ=255)
                                                   cATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCa                                                                                 <  8:123594/135‑1 (MQ=255)
                                                     ttCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCACGCCGGTGGGGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAgg                                                                               <  1:48404/135‑1 (MQ=255)
                                                      tCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGa                                                                              <  1:11649/135‑1 (MQ=255)
                                                       cgccTCAGTGCGGCCCGGGTAACCCCCCACCAGGCCCCGGAAACGCCACCCCCCCCCGGCACGCGGGTGGGCCCGACGCAGCCTG‑GTCCGGGCTAAAGGGCAATTGACGTCACGCGCCAGCGCGACCCGCAgg                                                                               <  2:352811/130‑1 (MQ=255)
                                                                    ccccGGTAACCTCGCAACAGCACCCGGTCAAGCCAACCCCCGACGGCAAGCCGGTGGTGCCGACGCAGCCTGCGGCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTtgc                                                                <  6:150053/135‑1 (MQ=255)
                                                                       cGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCAGCAGCCGGCGTCC‑GGCGCAAGTGCCCTGGCCCGCACGCGCCAGCGCGCCCCGCCCGCGCAGGTATTGTtgctgc                                                             >  2:151451/1‑135 (MQ=255)
                                                                       cGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTtgctgc                                                             >  8:309007/1‑135 (MQ=255)
                                                                            aCCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCCGCCTGCGTCC‑CGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGCCCCCCAGGAGCAGGTATTGTTTCTGcgcgc                                                         >  6:261652/1‑133 (MQ=255)
                                                                            aCCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCAGCCTGCGTCC‑CGCGCAAGTGCCATTGACCTCAACCGCCAGCGCCCCCCGCACGCGCAGGTAATGTTGCTGCGCGAt                                                        >  8:70565/1‑135 (MQ=255)
                                                                            aCCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTAAAGCCGGTGGTGCCGCCGCAGCCTGCGTCC‑GGCGAAAGTGACATTTAACTCAAGCGCCAGAGCGACCCGCAGGATCAGGTATTGTTGCTCCGCGAt                                                        >  8:160092/1‑135 (MQ=255)
                                                                                      cggACCCGGCCAAGCCAACCCCCGACGGCCAGCCGGTGGTGCCTCCGCAGCCTGCGTCC‑GCCGAAAGGGCCATTGACCTCCAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGcgcc                                              <  3:265712/134‑1 (MQ=255)
                                                                                           ccGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCAGCCGGCGTCC‑GGCGCAAGTGCCATTGCCCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTTCTGCGCGATGAACAGCgccgcccg                                         >  7:184190/1‑133 (MQ=255)
                                                                                           ccGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCAGCCAGCGTGC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCCCCCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCgccgcccg                                         >  4:9436/1‑133 (MQ=255)
                                                                                           ccGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCCGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCcag                                         >  1:291641/1‑135 (MQ=255)
                                                                                           ccGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCgccgcccg                                         >  3:41513/1‑133 (MQ=255)
                                                                                            cGGTCAACCCACCCCCCCCCGCCACGCCGGGGGTGCCGACGCCGCCGGCGCCC‑GGCGAACGTGCCATTGCCCCCACGCGCCCGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCcagc                                        <  4:140041/135‑1 (MQ=255)
                                                                                                cAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGCCCTCAACCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAgcagca                                    >  2:50873/1‑135 (MQ=255)
                                                                                                cAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCCGCGCGCCCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAgcagca                                    >  7:249756/1‑135 (MQ=255)
                                                                                                 acgCCAACCCCCCCCGCCACGCCGGGGGGGCCGACGCCGCCTCCGGCC‑GGCGAAAGGGCCATTGACCTCAAGCGCCACCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCaa                                   <  5:91649/133‑1 (MQ=255)
                                                                                                 aaGCCACCCCCCGCCGCCACGCGGGGGGTGCCGCCGCCGCCGGCGCCG‑GGCGAAAGGGCCATTCCCCTCACGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCaa                                   <  6:315866/135‑1 (MQ=255)
                                                                                                  aGCCAACCCCCGACGTCAAGCCGGTGGTGCCGCCGCAGCCGGGGTCC‑GGCGAAAGTGCCATTTCCCTCAAGCGCCAGCGCGCCCCGCCGGAGCAGGTATTTTTTCTGCGCGATGAACAGCGCCGCCCGCCGCAAc                                  >  6:217126/1‑135 (MQ=255)
                                                                                                           ccGACGTCAAGCCGGTGGTGCCGCCGCCGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCACCGCGACCCGCACGACCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCCACGCCGGACCa                         >  2:14250/1‑135 (MQ=255)
                                                                                                           ccGACGTCAAGCCGGTGGTGCCGCCCCCGCCTGCGTCC‑GGCGAAAGTGCCAATGACCTCCAGCGCCAGCCCGACCCGCACGAGCAGGTATTGTTGCTGCGCGCTGCACAGCCCCGCCCGCAGCAACGCCCGaa                           >  7:371705/1‑133 (MQ=255)
                                                                                                           ccGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGGATTGTTGCTGCGCGCTGAACATCGCCGCCCGCAGCAACGCCGGag                           >  6:186770/1‑132 (MQ=255)
                                                                                                                gTCAAGCCGGTGGTGCCGCCGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCCGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGc                    >  6:334540/1‑135 (MQ=255)
                                                                                                                 tCAAGCCGGTGGTGCCGCCGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCCGCGCGACCCGCAGGCGCAGGTATTGTTTCTGCGCGATGAACAGCGCCGCCCGCAGCAACGCCGGAACAAggg                     >  1:378778/1‑133 (MQ=255)
                                                                                                                    aGCGGGGGGTGCCGAGGCCGCCTGCGCCG‑GGCTAAAGTGCCATTGACCTCAACCGCCCGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGAGc                <  7:177479/135‑1 (MQ=255)
                                                                                                                    aGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGa                  >  8:85370/1‑133 (MQ=255)
                                                                                                                    aGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGa                  >  8:90530/1‑133 (MQ=255)
                                                                                                                      ccGGGGGTGCCGCCGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGAAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGAGCAg              <  4:299227/135‑1 (MQ=255)
                                                                                                                      ccGGGGGTCCCTACGCCGCCTGGGGCG‑GGCTAACGGCCCATTGACCTCACGCGCCCCCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGAGCAg              <  3:353071/135‑1 (MQ=255)
                                                                                                                      ccGGGGGGGCCGACTCTGCCTGCGTCC‑GGCTAACTTGCATTTTCCCTCACGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCACCGCCAGCAGCAACGCCGGAACAAGGGCGAGCAg              <  2:134663/135‑1 (MQ=255)
                                                                                                                               ccGACGCAGCCTGCGGCC‑GGCGAATGTGCCATTGACCTCAACCGCCAGCGCGACCCACAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGAGCAGGAACGCTAc     <  5:148866/135‑1 (MQ=255)
                                                                                                                                  aCGCAGCCGGCGTCC‑GGCGAAAGTGCCATTGACCTCACGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGAGCAGGAACGCTACGAg  <  6:317383/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
CTGCGCCTGCCGATACAGGGGGCATAGGAGAACGTCCATGCTGCCGCCGATCATTCCGCTCAGTGCCGCCCCGGTAACCTCGCAACAGGACCCGGTCAAGCCAACCCCCGACGTCAAGCCGGTGGTGCCGACGCAGCCTGCGTCC‑GGCGAAAGTGCCATTGACCTCAAGCGCCAGCGCGACCCGCAGGAGCAGGTATTGTTGCTGCGCGATGAACAGCGCCGCCAGCAGCAACGCCGGAACAAGGGCGAGCAGGAACGCTACGAG  >  ACB122/1308582‑1308846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑