| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,309,442 | C→T | 10.2% | intergenic (+12/‑194) | CJ019_01229 → / → aaeB_2 | hypothetical protein/p‑hydroxybenzoic acid efflux pump subunit AaeB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,309,442 | 0 | C | T | 10.2% | 110.6 / 4.1 | 39 | intergenic (+12/‑194) | CJ019_01229/aaeB_2 | hypothetical protein/p‑hydroxybenzoic acid efflux pump subunit AaeB |
| Reads supporting (aligned to +/- strand): ref base C (14/21); new base T (2/2); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01 | |||||||||||
GTTCTTCGACAACGTGGTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCG > ACB122/1309316‑1309568 | gTTCTTCGACAACGTGGTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCAc < 1:111767/135‑1 (MQ=255) tcttctACAACGTGGTTATCGCCGGGGTGGGAAGGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTATGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGTGTACACGAGCACg < 4:141107/134‑1 (MQ=255) tcttcGACAACGTGGTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACg < 5:163929/134‑1 (MQ=255) ggTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAggg > 3:121521/1‑135 (MQ=255) ccGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGCCTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCg > 1:337966/1‑135 (MQ=255) aaCGGGCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCAtt < 5:357679/135‑1 (MQ=255) gggCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTg > 4:171699/1‑135 (MQ=255) gggCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTg < 3:288999/135‑1 (MQ=255) gggCTGATGGTCGCCTTCTTTGCCGGTCTGGGCAATTTAATCTGGAAGGCCTCGAACAACCGCAAGCCCCCCTGACCATGCCGGTACACGAGCACCCAAGGCAATTAGGGCGACGTCGGGCGCCCCAATTTTTTg > 2:193475/1‑135 (MQ=255) gCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGcc < 2:323294/135‑1 (MQ=255) cTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCt > 7:311279/1‑135 (MQ=255) tGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCt > 5:55938/1‑134 (MQ=255) gATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCGCATTTTTTTTGCCTGc > 1:219011/1‑135 (MQ=255) tCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCAttt < 8:244317/134‑1 (MQ=255) ccGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAACCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCAttt < 5:292651/133‑1 (MQ=255) cGCCTTCTTTGCCGGTCTGGGCATTTTAATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCAGTTTTTTTTCCTGCAttttt > 3:224394/1‑135 (MQ=255) cttcttTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTAt < 8:78602/135‑1 (MQ=255) tttGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTg < 8:351750/133‑1 (MQ=255) ttGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTc > 4:356335/1‑134 (MQ=255) gCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt < 7:192216/133‑1 (MQ=255) gCCGGTCTGCGCATTTTACTATGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt < 7:150365/133‑1 (MQ=255) ccGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGa < 3:356335/134‑1 (MQ=255) ggTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCt < 7:232318/135‑1 (MQ=255) ggTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCt < 6:55938/135‑1 (MQ=255) gggCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTccc < 8:374925/135‑1 (MQ=255) aTTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCAATTTTTTTGCGTGAATTTTTTATTATCTGAGGTGTCGCtt > 6:287707/1‑133 (MQ=255) cATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTATACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt < 3:290841/116‑1 (MQ=255) cATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTATACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt < 3:285737/116‑1 (MQ=255) cATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTATACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt > 4:290841/1‑116 (MQ=255) cATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTATACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCt > 4:285737/1‑116 (MQ=255) tGGAAGGACTCGAACAAGCCCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAATGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGc < 6:60599/135‑1 (MQ=255) ggAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGc > 4:96388/1‑134 (MQ=255) cgACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATc < 3:25721/134‑1 (MQ=255) cGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAat > 8:306448/1‑134 (MQ=255) cgCCGCGCTGACCTTGCGGGTACACGAGCACTCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATtagc < 5:287707/134‑1 (MQ=255) aGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATtagc < 2:34341/135‑1 (MQ=255) tGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCtaa > 6:282028/1‑134 (MQ=255) gACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCACTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCtaat > 1:60130/1‑134 (MQ=255) cGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCg < 3:218425/133‑1 (MQ=255) acacGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAgg < 2:339804/91‑1 (MQ=255) acacGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAgg > 1:339804/1‑91 (MQ=255) | GTTCTTCGACAACGTGGTTATCGCCGGTGTGGTAACGGTCGGGCTGATGGTCGCCTTCTTTGCCGGTCTGGGCATTTTCATCTGGAAGGACTCGAACAAGCGCAAGCCGCGCTGACCTTCCGGGTACACGAGCACGCAAGGCATTTAGGGCGACTTCGGTCGCCCATTTTTTTTGCCTGCATTTTTTATTGTCTGAGCTGTCCCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCG > ACB122/1309316‑1309568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |