| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 63,547 | C→T | 6.2% | Y61Y (TAC→TAT) | tag → | DNA‑3‑methyladenine glycosylase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 63,547 | 0 | C | T | 6.2% | 186.5 / 2.9 | 65 | Y61Y (TAC→TAT) | tag | DNA‑3‑methyladenine glycosylase 1 |
| Reads supporting (aligned to +/- strand): ref base C (32/28); new base T (2/2); total (35/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGACCAGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGCCCAGGCCTGGCTGGCT > ACB122/63414‑63676 | aCGACCAGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCGCGAAGGGTTCCAGGCGGGGCGTTCGTGGATTACCGTTTTGCGCAAACGCGCGCGTTATCGCCAGGTGCTGTACg > 6:281315/1‑135 (MQ=255)aCGACCAGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCGTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACg > 2:95177/1‑135 (MQ=255)aCGACCAGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGACATGCTTTTTCTCGAAGGGTTCCAGGCGGGGCTTTAGTGGATTAACGTTTTGCGCAAACGCGAGCGTTATATCCAGGTGATTTACg > 5:278225/1‑135 (MQ=255) ccAGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCtt < 5:139516/135‑1 (MQ=255) ccAGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCtt < 3:15090/135‑1 (MQ=255) aGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCCGGCGGGGCTTTCGTGGATTACCCTTTTGCGCAAACGCGAGCGGTATCGCCAGGTGCTGTAAGGCTTCg > 1:149868/1‑135 (MQ=255) aGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCg > 6:211715/1‑135 (MQ=255) aGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCg > 2:263050/1‑135 (MQ=255) aGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGATTCg > 6:247627/1‑135 (MQ=255) aGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCGTTCGTGGATTACCGTGTTGCGCAAACGCGCGCGTTATCGCCAGGTGATGTACGGCTTCg > 6:224922/1‑135 (MQ=255) ggAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCgc > 8:358599/1‑134 (MQ=255) gAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCATTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTaa > 6:331119/1‑135 (MQ=255) tGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGc < 7:335847/135‑1 (MQ=255) ccACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTg < 2:172267/135‑1 (MQ=255) cacaGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGGTTTCGTGGTTTACCGGTTTGCGCAAACGCGAGCGGTTTCGCCAGGTGCTGTACGGCTTCGACCCGGTAAAGATgg > 2:358467/1‑135 (MQ=255) cacaGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGCCCCGGTAAAGCTgg > 2:128614/1‑135 (MQ=255) cacaGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTgg > 4:224224/1‑135 (MQ=255) cacaGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGGGGATTACCGTTTTGGGCAAACGCGAGCGTTTTCGCCAGGTGAAGTTCGGCTTCGACCCGGTTAAGCTgg > 1:287416/1‑135 (MQ=255) acaGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTggc > 8:240122/1‑135 (MQ=255) cgcgACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTATGGCTTCGACCCGGTAAAGCTGGCGGcc > 2:278462/1‑135 (MQ=255) cgcgACCCGGCGTTGCTCTTAGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTATGGCTTCGCCCCGGTAAAGCTGgccgcc > 2:38978/1‑135 (MQ=255) gACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATAGCCAGGTGATGTACGGCGTCGACCCGGTAACGCTGGCGGCCa > 1:137544/1‑133 (MQ=255) ggCGTTGCTCTTAGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGGTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGGGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGcga > 3:224180/1‑135 (MQ=255) ttGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGaa < 3:267933/135‑1 (MQ=255) tctagaTGCTTTTGCTCGAAGGGTTCCAGCCGGGGCTTTCGTGGATTACCGTTTTGCTCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCTGTAAAGCTGGCGGCCATGAGCGACGAACGCAt < 5:258390/130‑1 (MQ=255) cGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCga > 6:67005/1‑135 (MQ=255) gaTGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCgaaga > 5:102253/1‑135 (MQ=255) ttttGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTg < 5:211715/134‑1 (MQ=255) tttGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTgat < 6:278225/135‑1 (MQ=255) tttGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTgat < 4:220921/135‑1 (MQ=255) tGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTgat < 1:95177/133‑1 (MQ=255) tGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTgat < 1:218821/133‑1 (MQ=255) tGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACCCATCGAAGAATTgat < 7:93493/133‑1 (MQ=255) tCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTgatgcagg < 3:26507/135‑1 (MQ=255) tCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTgatgcagg < 4:66599/135‑1 (MQ=255) cAGGCGGGGCTTTCGTGGATTACCGCTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGcat < 4:115813/134‑1 (MQ=255) ggCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGcatcat > 3:41257/1‑135 (MQ=255) tttCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATc > 5:125156/1‑135 (MQ=255) cGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGc < 3:224224/134‑1 (MQ=255) cGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGc < 3:186281/134‑1 (MQ=255) cGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGc < 1:349943/134‑1 (MQ=255) gTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGcc > 8:263204/1‑134 (MQ=255) ggATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCct > 3:293986/1‑133 (MQ=255) tACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTgat < 7:301335/99‑1 (MQ=255) tACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTgat > 8:301335/1‑99 (MQ=255) tACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGACATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCctcaagc > 8:132679/1‑134 (MQ=255) tGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGcc < 4:370722/134‑1 (MQ=255) gcgcAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAACGCGGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCg < 6:335845/134‑1 (MQ=255) gcAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCaa > 3:20579/1‑135 (MQ=255) aaaCGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAgg > 7:121249/1‑135 (MQ=255) aaaCGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAgg > 1:309419/1‑135 (MQ=255) aaCGCGAGCGTTATCGCCCGGTTATGTACGGCTTCGACCCGGTAAAGCTGGCGGTCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGgc < 3:330519/135‑1 (MQ=255) aaCGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGgc < 4:41257/135‑1 (MQ=255) aaCGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGgc < 7:263204/135‑1 (MQ=255) gAGCGTTATCGCCAGGTGATGTATGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAAc < 1:278462/135‑1 (MQ=255) gAGCGTTATCGCCAGGGTATTTATGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAAc < 1:38978/135‑1 (MQ=255) gAGCGCTACCGCCCGGGGATGTACGGATTCCACCCGGTAACGCTGGCGGCCATGAGCCACGAACGCATCGAAGAATTGATGCAGGATGCAGGCAGCATCCGCAATCGCCGCAAGCTCAAGGCCGCAAGGCGCAAc < 6:246272/135‑1 (MQ=255) gTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGc > 1:205625/1‑133 (MQ=255) gTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGc > 5:178142/1‑133 (MQ=255) gCCCGGTTAGGTCCGGCTTCGCCCCGGTAAAGCGGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGCCCAGGCct < 5:255377/135‑1 (MQ=255) ccAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGGAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGCCCAGGCctg > 3:18894/1‑135 (MQ=255) aGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGCCCAGGCctggc < 5:247627/135‑1 (MQ=255) gggATGTACGGCGGCGACCCGGTAAAGCTGGCGGCCATGCGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCGCAAGGCCGCAAGGCGCAACGCCCAGGCctggctg < 5:224922/133‑1 (MQ=255) tGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGCCCAGGCctggctgg > 1:268873/1‑135 (MQ=255) aTGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGCCCAGGCCTggctggct > 8:375599/1‑135 (MQ=255) | ACGACCAGGAATGGGGAATGCCACAGCGCGACCCGGCGTTGCTCTTCGAGATGCTTTTGCTCGAAGGGTTCCAGGCGGGGCTTTCGTGGATTACCGTTTTGCGCAAACGCGAGCGTTATCGCCAGGTGATGTACGGCTTCGACCCGGTAAAGCTGGCGGCCATGAGCGACGAACGCATCGAAGAATTGATGCAGGATGCAGGCATCATCCGCAATCGCCTCAAGCTCAAGGCCGCAAGGCGCAACGCCCAGGCCTGGCTGGCT > ACB122/63414‑63676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |