Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,310,781 C→T 5.9% T382T (ACC→ACT)  aaeB_2 → p‑hydroxybenzoic acid efflux pump subunit AaeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,310,7810CT 5.9% 226.3 / 2.7 68T382T (ACC→ACT) aaeB_2p‑hydroxybenzoic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base C (22/42);  new base T (2/2);  total (24/44)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGGGCTGGCCGCGCACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGAGCAGCTT  >  ACB122/1310648‑1310915
                                                                                                                                     |                                                                                                                                      
aGGGGCTGGCCGCGCACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCt                                                                                                                                       <  3:40792/135‑1 (MQ=255)
 ggggCTGGCCGCGCACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTg                                                                                                                                      <  4:109264/135‑1 (MQ=255)
 ggggCTGGCCGCGCACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTg                                                                                                                                      <  4:285447/135‑1 (MQ=255)
 ggggCTGGCCGCGCACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTg                                                                                                                                      <  7:37934/135‑1 (MQ=255)
 ggggCTGGCCGCGCACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTg                                                                                                                                      <  5:342338/135‑1 (MQ=255)
 ggggCTGGCCGCGCACCGAGACTGGTCGCGGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTg                                                                                                                                      <  4:57540/135‑1 (MQ=255)
        gCCGCGCCCCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTTGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCGGCGTGGTc                                                                                                                               >  8:23162/1‑135 (MQ=255)
         ccGCGCTCCGAGAGTGGTCGCTGGCCGGGCTGTTCGGCTCGCGCAGCGCGGTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCt                                                                                                                              <  7:361474/135‑1 (MQ=255)
         ccGCGCACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCt                                                                                                                              <  7:361472/135‑1 (MQ=255)
         ccGCGCACCGAGACTGGTCGCTGGCCTTGCGGTGCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGGGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCt                                                                                                                              <  3:88282/135‑1 (MQ=255)
           gcgcACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGGAATGAGTGGTTTGTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCGGGTGCTGCCCTGCGTGGGCt                                                                                                                              >  8:155872/1‑133 (MQ=255)
                 cGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCGTCCGCCCCCGGTGGCCGGGTGCTGACCTGCGTGGTCTGCAGccgg                                                                                                                      >  4:13874/1‑133 (MQ=255)
                  gagaCTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGACTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGt                                                                                                                     <  5:376605/135‑1 (MQ=255)
                     aCTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCg                                                                                                                  <  5:146316/135‑1 (MQ=255)
                           cGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCCGCCTGTTCGCCAGTc                                                                                                            >  7:249836/1‑135 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGGCGTGCGCCCCCCGGGGCGGGGTGGTGAGCGGCGGGGGGGGCACCCCGTTGGCCACTcg                                                                                                           >  5:37839/1‑135 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTg                                                                                                                                      >  3:14986/1‑108 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTg                                                                                                                                      <  4:14986/108‑1 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTcg                                                                                                           >  2:198853/1‑135 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTcg                                                                                                           >  2:204948/1‑135 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTcg                                                                                                           >  5:256805/1‑135 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTcg                                                                                                           >  3:104327/1‑135 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTcg                                                                                                           >  1:251394/1‑135 (MQ=255)
                            gCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCAGGTGCTTACCTGCGTGGTCTGCCGCCAGTTCGCCCGTcg                                                                                                           >  6:40147/1‑135 (MQ=255)
                             cTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGc                                                                                                                            >  8:364098/1‑117 (MQ=255)
                             cTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGc                                                                                                                            <  7:364098/117‑1 (MQ=255)
                                 ccTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCga                                                                                                        <  3:93762/133‑1 (MQ=255)
                                 ccTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCga                                                                                                        <  7:375375/133‑1 (MQ=255)
                                  cTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACTTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGaa                                                                                                     >  2:30643/1‑135 (MQ=255)
                                  cTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAAAGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCGGGTGCTGACTTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGaa                                                                                                     >  2:30655/1‑135 (MQ=255)
                                      cTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACgg                                                                                                  <  1:119540/134‑1 (MQ=255)
                                      cTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACg                                                                                                   <  2:105799/133‑1 (MQ=255)
                                       tGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGgcg                                                                                                >  8:198813/1‑135 (MQ=255)
                                              cTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGGCGTCGGCCCCCGGTGGCCTGGTGATGCCCTACGTTGTATGCCGCCTGGTCGCCATTCGCGAGAACTGCGcacatcg                                                                                         >  6:29596/1‑132 (MQ=255)
                                              cTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGGCGTCGGCCCCCGGGGGGCGGGGGCTGACCTGGGTGGGGTGCAGCCCGTTGGCCCGTCGCGCGCACCGCGcccacct                                                                                         >  2:179518/1‑132 (MQ=255)
                                                  cgcAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCg                                                                                       <  2:211026/133‑1 (MQ=255)
                                                  cgcAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCg                                                                                       <  5:288104/133‑1 (MQ=255)
                                                  cgcAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCg                                                                                       <  8:158015/133‑1 (MQ=255)
                                                   gcAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGgc                                                                                                 >  4:81846/1‑122 (MQ=255)
                                                   gcAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGgc                                                                                                 <  3:81846/122‑1 (MQ=255)
                                                             ggCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCt                                                                          <  7:198813/135‑1 (MQ=255)
                                                               ccTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCt                                                                          <  7:269829/133‑1 (MQ=255)
                                                                cTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGTGCACAGATCGGCCTGAGTTTCCTgcg                                                                       <  6:70145/135‑1 (MQ=255)
                                                                    cTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGcgcg                                                                     <  5:225487/133‑1 (MQ=255)
                                                                           tGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTcc                                                            >  4:321519/1‑135 (MQ=255)
                                                                              gTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTgg                                                         <  4:105872/135‑1 (MQ=255)
                                                                                    tCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGCTAc                                                   >  1:224037/1‑135 (MQ=255)
                                                                                     ctggctggCCACGGCCTGGCCTTCGGCCCCCGGGGGCCTGGTGCTGACCGGCGTGGTCTGCAGCCAGTTCGCCACTCGCGAGCACCGCGCCCAGATCGGCCTGAGTTTCCTTCGCCGGTTATTCCGGGCGGTAcc                                                  >  6:253217/1‑135 (MQ=255)
                                                                                      tggctggcCACGGCCTGACCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACcg                                                 <  3:249213/135‑1 (MQ=255)
                                                                                         ctggcCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACcggcg                                              >  7:67326/1‑135 (MQ=255)
                                                                                         ctggcCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACcg                                                 <  8:42403/132‑1 (MQ=255)
                                                                                         ctggcCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACcg                                                 <  6:256805/132‑1 (MQ=255)
                                                                                         ctggcCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACcg                                                 <  5:40147/132‑1 (MQ=255)
                                                                                         ctggcCACGGCCTGGCCTTCGGCCCCCGGTGGACTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGTCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACcg                                                 <  6:362513/132‑1 (MQ=255)
                                                                                                    ctggcctTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGt                                   <  3:13874/135‑1 (MQ=255)
                                                                                                     tggcctTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTc                                  <  7:114633/135‑1 (MQ=255)
                                                                                                     tggcctTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTc                                  <  2:140399/135‑1 (MQ=255)
                                                                                                         ctTCGGCCCCCGGTGGCCTGGTGCTGCCCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCgg                                >  8:105561/1‑133 (MQ=255)
                                                                                                               cccccGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCa                        <  6:348011/135‑1 (MQ=255)
                                                                                                                  ccGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACAGGCGGCGTTTGTGGTCGGGCAGATCAtgc                     <  2:52686/135‑1 (MQ=255)
                                                                                                                       ggCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCg                <  8:287/135‑1 (MQ=255)
                                                                                                                       ggCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGGTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCg                <  4:104327/135‑1 (MQ=255)
                                                                                                                          cTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCaa             <  6:316091/135‑1 (MQ=255)
                                                                                                                              tGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATg           <  5:375754/133‑1 (MQ=255)
                                                                                                                                 tGACTTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGagca      <  1:30655/135‑1 (MQ=255)
                                                                                                                                 tGACTTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGagca      <  1:30643/135‑1 (MQ=255)
                                                                                                                                 tGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGagca      <  5:61933/135‑1 (MQ=255)
                                                                                                                                 tGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGagca      <  1:179518/135‑1 (MQ=255)
                                                                                                                                     cTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGAGCAGCtt  >  8:10631/1‑135 (MQ=255)
                                                                                                                                     cTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGAGCAGCtt  <  1:71475/135‑1 (MQ=255)
                                                                                                                                     cTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGAGCAGCtt  <  1:204948/135‑1 (MQ=255)
                                                                                                                                     cTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGAGCAGCtt  <  1:198853/135‑1 (MQ=255)
                                                                                                                                     cTGCGTGGGCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGAGCAGCtt  <  6:116247/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
AGGGGCTGGCCGCGCACCGAGACTGGTCGCTGGCCTTGCTGTTCGGCTCGCGCAGCGCGCTGGCCTTCCTGGTAATGAGTGGTTTCTGGCTGGCCACGGCCTGGCCTTCGGCCCCCGGTGGCCTGGTGCTGACCTGCGTGGTCTGCAGCCTGTTCGCCAGTCGCGAGAACGGCGCACAGATCGGCCTGAGTTTCCTGCGCGGGATATTCCTGGCGATACCGGCGGCGTTTGTGGTCGGGCAGATCATGCTGCCGCAATGGAGCAGCTT  >  ACB122/1310648‑1310915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑