Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,463,765	C→T	6.5%	V196V (GTG→GTA)	CJ019_01381 ←	Mannuronan synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,463,765	0	C	T	6.5%	166.9 / 3.7	61	V196V (GTG→GTA)	CJ019_01381	Mannuronan synthase
Reads supporting (aligned to +/- strand):  ref base C (35/22);  new base T (2/2);  total (37/24)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

AAGGTTGCCCTTGAGCATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCTTGAAGCGGCCGAAGGC  >  ACB122/1463633‑1463899                                                                                                                                     |                                                                                                                                       aaGGTTGCCCTTGAGCATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCAc                                                                                                                                      >  8:293002/1‑135 (MQ=255)  aGGTTGCCCTTGAGCATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCCCt                                                                                                                                     >  3:372243/1‑135 (MQ=255)    gTTGCCCTTGAGCATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGc                                                                                                                                   <  8:284030/135‑1 (MQ=255)          cTTGAGCATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGCCCAGGCTGCTGACGGTCCCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACGGCGACcac                                                                                                                             >  5:311638/1‑135 (MQ=255)          cTTGAGCATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACcac                                                                                                                             >  5:86213/1‑135 (MQ=255)          cTTGAGCATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACcac                                                                                                                             >  7:40787/1‑135 (MQ=255)                cATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTAACGGTACCGTCGCGCGGCAGGGTGACGTTGGTGGTCGGCACAGCGACCACACCgg                                                                                                                        >  1:344332/1‑134 (MQ=255)                     ccAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCGTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGCTGACGTTGGTGGTCGGCACTGCCACCACCCCGGCTTCgg                                                                                                                  >  1:82969/1‑135 (MQ=255)                      cAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCCCACCGGCTTCGGc                                                                                                                 >  7:58169/1‑135 (MQ=255)                      cAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGc                                                                                                                 >  1:325202/1‑135 (MQ=255)                              tggtggTGACGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCAAGCGCTTTCGACCAGGGTGCTTACGGTCCCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGGGGCTg                                                                                                          <  4:305638/134‑1 (MQ=255)                                gtggtgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGGGGCATGGTGCCGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCGGAc                                                                                                        >  1:106602/1‑134 (MQ=255)                                 tggtgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGTACTGCGACCACACCGGCTTCGGCGTGGCTGACg                                                                                                       >  1:278563/1‑134 (MQ=255)                                 tggtgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGTACTGCGACCACACCGGCTTCGGCGTGGCTGACg                                                                                                       >  1:283705/1‑134 (MQ=255)                                 tggtgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCCCTGCGACCCCACCCGCTTCGGCGTGGCTGACg                                                                                                       >  4:195473/1‑134 (MQ=255)                                 tggtgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCCCCCCGGCTTCGGCGTGGCTGACg                                                                                                       >  1:62200/1‑134 (MQ=255)                                 tggtgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGCCg                                                                                                       >  5:207176/1‑134 (MQ=255)                                 tggtgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGa                                                                                                      <  2:325202/135‑1 (MQ=255)                                   gtgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCCGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGCCGaa                                                                                                     >  4:166159/1‑134 (MQ=255)                                    tgAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAAt                                                                                                   <  3:199119/135‑1 (MQ=255)                                      aaGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATAc                                                                                                 >  1:260199/1‑135 (MQ=255)                                         cTGGCCAATGGCGCGCCCTTGGCGCTCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCCCACCGGCTGCGGCGTGGCTGACGCAATACCTg                                                                                              >  4:137454/1‑135 (MQ=255)                                             ccAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCCCAGCGACCCCCCCCGCTTCGGCGGGGCGGACGAAATACATGccca                                                                                          >  7:150456/1‑133 (MQ=255)                                               aaTGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACTGTACCGTCGCGCGGCTTGGTGAAGTTGGTGGTCGGCACTGCGACCACCCCGGCTTCGGCGTGGCTGACGAAATACAAGCCCTa                                                                                         >  5:238610/1‑134 (MQ=255)                                                         ccTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTgg                                                                              <  5:137763/135‑1 (MQ=255)                                                         ccTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTAGGCACTGCGCCCCCACCGGCTTAGGCGTGGCTGCCGAAATACATGCCATACAACGAATTgg                                                                              >  7:37105/1‑135 (MQ=255)                                                           ttGGCGATCTGCCCAGCGCTTTCGACCAGGCTGCTTACTGTAGCGTCGGGCGGGATGGTGACGTTGGTGGGCGGCACTGCGACCACACCGGGTTCGGCGTGGCTGACGAAATACATGCCATACAACTATTTGGcg                                                                            <  2:344332/135‑1 (MQ=255)                                                           ttGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGGGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGcg                                                                            <  6:231304/135‑1 (MQ=255)                                                                      cccACCGCTTTCGACCAGGCTGCTTAAGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCCCACCCGCTTCGGCGTGGCTGCCGAAATACATGCCAAACAACGAATTGGCGACGCAGCCCaa                                                                 >  8:63531/1‑135 (MQ=255)                                                                       ccACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGcc                                                                    <  6:272212/131‑1 (MQ=255)                                                                        cACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAgg                                                               >  4:156640/1‑135 (MQ=255)                                                                                   aCCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCg                                                    >  5:343240/1‑135 (MQ=255)                                                                                   aCCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCCAAGGCCGCAACCCCg                                                    >  6:377867/1‑135 (MQ=255)                                                                                        gctgctTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCAc                                               <  2:260199/135‑1 (MQ=255)                                                                                        gctgctTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGGGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCAc                                               <  8:40787/135‑1 (MQ=255)                                                                                         ctgctTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACg                                              <  3:156640/135‑1 (MQ=255)                                                                                           gctTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCTCAACGCCGACCACGaa                                            >  8:182384/1‑135 (MQ=255)                                                                                           gctTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGccccccc                                             >  1:120348/1‑130 (MQ=255)                                                                                           gctTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGaa                                            >  4:114584/1‑135 (MQ=255)                                                                                              aaCGGTACCGTCGCGCGGCATGGGGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACCTGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAacac                                         <  8:307281/134‑1 (MQ=255)                                                                                                cGGTACGGGCGCGCGGCATGGTGACGTTGGTGGGCGGCACTGCGACCACACCGGCTTCGGCGGGGCTGACGAAATACATGCCATACAACGATTTGGCGACCAAGCCGAAGGCCGCAACGCCGACCACGAacac                                         <  3:195473/133‑1 (MQ=255)                                                                                                cGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAacac                                         <  4:219178/133‑1 (MQ=255)                                                                                                cGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCGGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAacac                                         <  4:219182/133‑1 (MQ=255)                                                                                                 ggTACCGTCGCGCGGCATGGTGACGGTGGTGGTCGGCACTGCGACCACACCGGCTGCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGc                                       <  7:211958/134‑1 (MQ=255)                                                                                                     ccGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCCCACCGGCTTCGGCGTGGCTGACGATATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCaga                                  >  3:315142/1‑135 (MQ=255)                                                                                                     ccGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGACGCCGAAGGCCGCAACGCCGACCACGAACACGCCCaga                                  >  8:330879/1‑135 (MQ=255)                                                                                                         cgcgcgGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCt                                                                                                           >  5:360901/1‑58 (MQ=255)                                                                                                         cgcgcgGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCt                                                                                                           <  6:360901/58‑1 (MQ=255)                                                                                                         cgcgcgGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGa                              >  7:370512/1‑135 (MQ=255)                                                                                                         cgcgcgGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCCGCGTGGCTGACGAAATACATGCCATACCACGATTTGGCGCCGAAGCCGAAGGCCGCAACGCCGACCACGAACCCGCCCAGAGTGa                              >  4:141442/1‑135 (MQ=255)                                                                                                            ggggCTTGGGGACGTGGGGGGGCGGCACTGCGACAACACCGGCTTCGGCGTGGCTGACTAAATACATGCCTTACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACgg                           <  6:27216/133‑1 (MQ=255)                                                                                                                      gACGTTGGTGGTCGGTACTGCTACCAAACCGGCTTCGGCGTGGCTGACTAAATACATGCCATACAACTATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCtt                 <  2:278563/135‑1 (MQ=255)                                                                                                                      gACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGCCGAAATACATGCCATACAACGATTTGGCGACGACGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCtt                 <  6:207176/135‑1 (MQ=255)                                                                                                                      gACGTTGGTGGGCGGTACTGCGACCACACCGGCTGGGGCGGGGCTGACGAAATACATGCCATCCAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCtt                 <  2:283705/135‑1 (MQ=255)                                                                                                                       aggTTGGTGGGCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACCAAATACATCCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACTAACACGCCCAGAGTGACGGTGACTGCCTTg                <  2:254040/133‑1 (MQ=255)                                                                                                                       aCGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCtt                 >  3:43133/1‑134 (MQ=255)                                                                                                                           tggtggTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGCAGCCGCAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCTTGaatc            >  6:22324/1‑133 (MQ=255)                                                                                                                             gtggtCGGCACTGCGCCCACACCGGCTTCGGCGTGGCTGCCGAAATAAAAGGCATACAACGATTTGGCGCCGCAGCCGCAGGCCGCAACGCCGCCCACGCACACG‑CCAGAGTGACGGTGACTGCCGTGAAGCGGc         >  6:47883/1‑135 (MQ=255)                                                                                                                             gtggtCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCCCGAACACGCCCAGAGTGACGGTGACTGCCTTGAAGCgg          >  4:71834/1‑135 (MQ=255)                                                                                                                                gtCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCTTGAAGCGGCCg       <  8:370512/135‑1 (MQ=255)                                                                                                                                gacGGCACTGCCACCACACCGGCTCCGGCGGGGCGGACGAAAACCATGCCAAACAACGATTGGGCGACGAAGCCCAAGGCCGCAACGCCTACCACTAACACGCCCAGAGTGACGGTGACTGCCTTGAAGCGGCCg       <  2:120348/133‑1 (MQ=255)                                                                                                                                     cACTGCGACCACACCGGCTGCGGCGTGGCTGACGAAATACATGCCATACAACGATTGGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCTTGAAGCGGCCGAAGgc  <  4:372243/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       AAGGTTGCCCTTGAGCATGTCCAGCATGCTGGTGGTGAAGCTGGCCAATGGCGCGCCCTTGGCGATCTGCCCACCGCTTTCGACCAGGCTGCTTACGGTACCGTCGCGCGGCATGGTGACGTTGGTGGTCGGCACTGCGACCACACCGGCTTCGGCGTGGCTGACGAAATACATGCCATACAACGATTTGGCGACGAAGCCGAAGGCCGCAACGCCGACCACGAACACGCCCAGAGTGACGGTGACTGCCTTGAAGCGGCCGAAGGC  >  ACB122/1463633‑1463899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑