Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,497,034	G→A	6.0%	R113H (CGT→CAT)	rpsI →	30S ribosomal protein S9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,497,034	0	G	A	6.0%	220.4 / 3.2	66	R113H (CGT→CAT)	rpsI	30S ribosomal protein S9
Reads supporting (aligned to +/- strand):  ref base G (29/33);  new base A (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

TGTCAGCGGTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGT  >  ACB122/1496909‑1497161                                                                                                                              |                                                                                                                                tgtCAGCGGTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGt                                                                                                                        <  5:129193/135‑1 (MQ=255) tggcAGCGGTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGt                                                                                                                        <  4:347454/132‑1 (MQ=255)  gtCAGCGGTCAGGCGGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTg                                                                                                                       <  5:365171/135‑1 (MQ=255)  gtCAGCGGTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGTTCGTGAAGTTGAGCGTAAGAAAGTg                                                                                                                       <  2:264336/135‑1 (MQ=255)  gtCAGCGGTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTg                                                                                                                       <  1:179670/135‑1 (MQ=255)  gtCAGCGGTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTg                                                                                                                       <  7:242226/135‑1 (MQ=255)        ggTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTg                                                                                                                 >  6:33634/1‑135 (MQ=255)         gTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGc                                                                                                                <  2:93957/135‑1 (MQ=255)             ggCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGt                                                                                                              >  6:373755/1‑133 (MQ=255)               ccGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAg                                                                                                          >  6:88443/1‑135 (MQ=255)                cGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGTCGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAgc                                                                                                         <  6:188868/135‑1 (MQ=255)                 ggTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAgcg                                                                                                        >  5:178175/1‑135 (MQ=255)                     cGATCCGTCACGGTATCACCCGCGCTCTGATGGAATAGGACGAAACCCTGCGTGGCTCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTa                                                                                                    <  2:104951/135‑1 (MQ=255)                     cGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTa                                                                                                    <  6:72106/135‑1 (MQ=255)                      gATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTa                                                                                                    <  4:142171/134‑1 (MQ=255)                        tCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGc                                                                                                 <  8:109398/135‑1 (MQ=255)                        tCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGc                                                                                                 <  8:317222/135‑1 (MQ=255)                        cccGGCACGGTAGCACCCGCGCTCTGCTGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGTCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGc                                                                                                 <  5:34012/134‑1 (MQ=255)                           gTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGAGGCGCTCTGCGACGTGCTGGCTACGTCACCCGCGACGCTCGGGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGt                                                                                               >  4:103224/1‑134 (MQ=255)                                     cACCCTCGAGCTGATTGAATACGACGAAACCCTGCTTGGCGCTCTGCGTCGTGGTGGATAGGTCACGCGCGACGCTCGTGAAGTTGAGCGTAAGATAGTGGGTCTGCGTAAAGCGCGTATGCGTCCTCAGTACt                                                                                     <  4:278594/134‑1 (MQ=255)                                     cACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACt                                                                                     <  1:15132/134‑1 (MQ=255)                                       cccGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTcc                                                                                   <  6:177071/134‑1 (MQ=255)                                         cgcgCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAg                                                                                >  6:282697/1‑135 (MQ=255)                                         cgcgCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAg                                                                                <  5:119331/135‑1 (MQ=255)                                             ctctGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGa                                                                                                                            >  5:374314/1‑87 (MQ=255)                                             ctctGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGa                                                                                                                            <  6:374314/87‑1 (MQ=255)                                             ctctGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGt                                                                             >  8:61341/1‑134 (MQ=255)                                             ctctGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGt                                                                             >  7:250909/1‑134 (MQ=255)                                               ctGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTa                                                                           >  5:121052/1‑134 (MQ=255)                                                     gAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTc                                                                                              >  6:28899/1‑109 (MQ=255)                                                     gAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTc                                                                                              <  5:28899/109‑1 (MQ=255)                                                           gacgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGa                                                                                                                            >  3:323176/1‑73 (MQ=255)                                                           gacgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGa                                                                                                                            <  4:323176/73‑1 (MQ=255)                                                           gacgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGc                                                              <  6:178175/135‑1 (MQ=255)                                                           gacgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGc                                                              <  1:65708/135‑1 (MQ=255)                                                           gacgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGc                                                              <  5:88443/135‑1 (MQ=255)                                                             cgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTGCTCAGTACTACAAGCGTTAATACCGCTTCGGCAg                                                            >  4:100993/1‑135 (MQ=255)                                                             cgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAg                                                            >  5:149826/1‑135 (MQ=255)                                                             cgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAg                                                            >  6:15096/1‑135 (MQ=255)                                                               aaaCCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGt                                                           >  8:50356/1‑134 (MQ=255)                                                                   ccTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGaa                                                       >  8:209018/1‑134 (MQ=255)                                                                        gTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGc                                                 >  4:48845/1‑135 (MQ=255)                                                                         tGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAACGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGcc                                                <  8:267281/135‑1 (MQ=255)                                                                          ggCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGt                                                                                                              <  2:294873/72‑1 (MQ=255)                                                                          ggCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGt                                                                                                              >  1:294873/1‑72 (MQ=255)                                                                                           tGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCggg                              >  3:73730/1‑135 (MQ=255)                                                                                           tGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCggg                              >  8:252159/1‑135 (MQ=255)                                                                                           tGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGGTCCTTGGAACCggg                              >  1:307481/1‑135 (MQ=255)                                                                                           tGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCCGTTCCGTGGAACCggg                              >  2:26566/1‑135 (MQ=255)                                                                                                aCGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCtttt                         >  4:355419/1‑135 (MQ=255)                                                                                                 cGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCttttt                        <  5:282697/135‑1 (MQ=255)                                                                                                  gTCACCCGCGACGCTCGTGAAGGTGAGCGTAAGAAAGTGGGGCTGCGTAACGAGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCTCTTCGGCAGTCGAAATAAGCCCGGTTCCTTGGAACCGGGCttttt                        <  8:218262/134‑1 (MQ=255)                                                                                                     aCCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCTTTAATACCGCTTCGGCAGTCGAAAAAAGCCCCGGTCCCTGGAACCGGGGgtttttt                      >  3:230976/1‑133 (MQ=255)                                                                                                      ccggcgAGGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCTTTAATACCGCTTCGGCAGTCTAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTa                   <  3:147246/132‑1 (MQ=255)                                                                                                      cccGCGACGCTCGTGAAGTTGAGCGTAAGAACGTGGGTCTGCGTAAAGCGCGTAAGCTTCCTCAGTTCTCCACGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTa                   <  3:260811/135‑1 (MQ=255)                                                                                                      cccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTa                   <  7:34302/135‑1 (MQ=255)                                                                                                      accccgacgaTCGTGAAGTTGCCCGTAAGAAAGGGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGGACTCCAATCGATAATACCGCTACGGCAGGCGAAAAAAGCCCGCCTCCTTGGAACCGGGCTTTTTTTTTa                   <  5:281195/131‑1 (MQ=255)                                                                                                        cgcgACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAGCCGGGCTTTTTTTTTTGc                 >  7:218262/1‑135 (MQ=255)                                                                                                          cgACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCCGGTGCGTGGGCCCGGGGgtgtttttttgggg               >  7:144909/1‑130 (MQ=255)                                                                                                          cgACGCTCGTGAAGTTGAGCATAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCtt                                                                  <  7:354643/84‑1 (MQ=255)                                                                                                          cgACGCTCGTGAAGTTGAGCATAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCtt                                                                  <  7:350079/84‑1 (MQ=255)                                                                                                          cgACGCTCGTGAAGTTGAGCATAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCtt                                                                  >  8:350079/1‑84 (MQ=255)                                                                                                          cgACGCTCGTGAAGTTGAGCATAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCtt                                                                  >  8:354643/1‑84 (MQ=255)                                                                                                                   tGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTaa       >  4:249557/1‑134 (MQ=255)                                                                                                                   tGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAACCCGGTTGCTTGGCACCGGGCGTTTTTTTTAGCCGTTAACTaaa      >  4:147246/1‑134 (MQ=255)                                                                                                                       gTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGt  <  4:191557/135‑1 (MQ=255)                                                                                                                              |                                                                                                                                TGTCAGCGGTCAGGCCGGTGCGATCCGTCACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGT  >  ACB122/1496909‑1497161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑