Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,718,476	T→G	22.2%	T61P (ACC→CCC)	CJ019_01614 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,718,476	0	T	G	22.2%	75.4 / 2.4	62	T61P (ACC→CCC)	CJ019_01614	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (12/34);  new base G (10/6);  total (22/40)
Fisher's exact test for biased strand distribution p-value = 1.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGA  >  ACB122/1718345‑1718608                                                                                                                                    |                                                                                                                                     cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCAc                                                                                                                                   >  7:92066/1‑135 (MQ=255) cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTcgc                                                                                                                                   >  1:187350/1‑133 (MQ=255)     ttGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCa                                                                                                                               <  8:317357/135‑1 (MQ=255)         cATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGGCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAgggg                                                                                                                           <  3:299517/135‑1 (MQ=255)              aGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCGGGGGGCGGCAGGGGCGTcc                                                                                                                      >  1:12390/1‑135 (MQ=255)                    ctGCGCGCACTACCCGCATTGCGGTGGCCAGGCTTGCCCTGCGCGGGGTCATAGGGGAGTTGGAACGGCAGCGGCACGTTGCCTTGGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGc                                                                                                                <  2:65935/134‑1 (MQ=255)                    aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGGGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                                <  7:296059/135‑1 (MQ=255)                    aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                                <  3:373363/135‑1 (MQ=255)                    aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                                <  7:202106/135‑1 (MQ=255)                       cgcgcACTTACCGCATAGCGGTGGGCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                                             <  7:24075/135‑1 (MQ=255)                       cgcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGGGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                                             <  7:79312/135‑1 (MQ=255)                       cgcgcACTTACCGCATAGCGGTGGCCAGGCGTGACCGGCGCGGGGGCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                                             <  5:86/135‑1 (MQ=255)                       cgcgaAATTACCGCATAGGGGGGGCCCGGCTTGACCGGGGGGGGGTCATCGGGGCGAGGCACCGGCCGCGGCACGTGGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGac                                                                                                             <  4:23378/135‑1 (MQ=255)                        gcgcACTTACCTCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTTGCGCGCCAGGGTCACCGCCGGGGCGTGCAGCcgcgcc                                                                                                             >  6:267052/1‑132 (MQ=255)                        gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGGGCGCCCGGGTCCCGGCAGGGGCGGCCAACAGCgccc                                                                                                            >  6:22373/1‑132 (MQ=255)                        gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCCGGGGCGGCCCTCAGCGcca                                                                                                            >  4:153257/1‑135 (MQ=255)                        gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCCCGGCCGGGGCGGCCCTCCGCGaca                                                                                                            >  5:66975/1‑135 (MQ=255)                         cgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGacac                                                                                                           <  8:57311/135‑1 (MQ=255)                            aCTTACCGATTAGCGGTGGCCAGGCTGGACGTGCGCGGGGTCATTGGTGACAGGGAACGGCAGCGGCAGGTTGCGTTTGACGGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATc                                                                                                        <  4:327797/135‑1 (MQ=255)                             cTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGGGACGGCCGGGGCGGGCCGCAGCGACCCCTcc                                                                                                       >  8:16813/1‑135 (MQ=255)                                  cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCCGGGGCGGCCCTCCGCGACCCCGCCTGCAg                                                                                                  >  8:202106/1‑135 (MQ=255)                                         cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                                            <  4:332367/134‑1 (MQ=255)                                         cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCCCGGCCGGGGCGGCCCTCCCCGCCACATCCGTCCGTTccccc                                                                                           >  2:346922/1‑132 (MQ=255)                                         cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGGGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACg                                                                                           <  2:281646/135‑1 (MQ=255)                                                 aGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGGCCCGGCGGGGGCGGCCCTCAGCGCCCCATCCTGCGGGGCCGCGCGCAGGGt                                                                                   >  3:325256/1‑135 (MQ=255)                                                     ttGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGCCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                                               <  7:130837/135‑1 (MQ=255)                                                     gtgcccTGCGGGGGGGGATGGGTGAGCTGCACCGGACGCGCCACGTGGCGGTTGCCCGGGCGGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGGCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                                               <  7:86637/131‑1 (MQ=255)                                                     gtGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                                               <  4:59614/134‑1 (MQ=255)                                                      tGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCCGGGGCGTCCCTCAGCGCCACCTCCTGCAGTTCCCCGCGCCGGGGggcgg                                                                              >  6:77998/1‑135 (MQ=255)                                                      tGACCTGCGCGGGGTCATAGGTGAGATGCAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcgg                                                                              <  5:267052/135‑1 (MQ=255)                                                          gtGCGCGGGGTCTTAGGTGACATGTAACGCCAGCGGCAGGTTGCCTTTGACCGGGCCGTCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                                          <  3:183853/134‑1 (MQ=255)                                                          cTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                                          <  8:265502/135‑1 (MQ=255)                                                          cTGCGCGGGGGCATAGGTGAGATGGACCGGCCGCGGCACGTTGCCTGTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                                          <  1:44959/135‑1 (MQ=255)                                                           tGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCGGGGCGCGGCCGGGGCGGCCCTCAGCGCCCCCGCCTGCAGGTCCCCCCTCCGGGGggcgggcggc                                                                         >  5:187422/1‑130 (MQ=255)                                                                ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCAGGCGCGCCACGGGCACCGCCGGGGCGTCCATCCGCGACACCTCCAGCAGTTACACGCTCACGGGggcggccggcggccg                                                                    >  3:107889/1‑133 (MQ=255)                                                                    tCATAGGTTAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCa                                                                <  3:129386/135‑1 (MQ=255)                                                                     cATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGGGGGCGCGCCCGGGGCCCGGCCCGGGGGGGGCGCAGCGACACATCCTGCCGGTACACGCGGAGGGTGGCGGCCGGCGGCCGGGcca                                                               >  8:244836/1‑133 (MQ=255)                                                                      aTAGGTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAAt                                                              <  3:184402/135‑1 (MQ=255)                                                                          gggagaGGGAACGGCAGCGCCACGTTGCCTTTGCCCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                                          <  2:187350/133‑1 (MQ=255)                                                                          gTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGGGACCGCAGGGGCGGCCCTCAGCGCCACAACCAGCCCTTCCCCGCGCAGGGTGGCGGCCCGCGGCAGGGCCATgcgc                                                          >  3:262796/1‑135 (MQ=255)                                                                          gTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                                          <  7:244836/135‑1 (MQ=255)                                                                                tGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCAGGGGCGGCCATCAGCGCCACAACCTGCACGTCCCCGCGCATGGTGGCGGCCGGCGGCCGGGCCATGCGCGGCAgg                                                    >  6:249962/1‑135 (MQ=255)                                                                                       gCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGGGCGGCCGGGGCCCGGCCGGGGGGGCCATCCGCGCCACAGCCGGCAGATCCCCGCGCCGGGGGGCGGGCGGCGGCCGGGCCATGCGCGGCAgggggcag                                              >  8:59111/1‑128 (MQ=255)                                                                                         cggggCAGGTTGCCTTTGCCGGGGCGGGCGGGGGGCGCCTGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                           <  2:180218/132‑1 (MQ=255)                                                                                         cggggCACGTGGCCTTTGACGGGGCCGGCGTGGTGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGTCAATGCGCTGCAGGTAGAAGACt                                           <  1:158365/132‑1 (MQ=255)                                                                                         cgCGGCAGGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                           <  5:344722/134‑1 (MQ=255)                                                                                         aGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                           <  2:31027/135‑1 (MQ=255)                                                                                         aGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                           <  5:189187/135‑1 (MQ=255)                                                                                         aGCGGCACGTTGCCGTTGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                           <  4:223204/135‑1 (MQ=255)                                                                                           cGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCAGGGGCGTCCATCAGCGCCCCAACCAGCAGTTCCACGCGCAGGGTGGCGGCCGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTc                                         >  6:186783/1‑135 (MQ=255)                                                                                                       tttGCCCGGGCCGGCTGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCt                             <  5:372116/135‑1 (MQ=255)                                                                                                          gACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTg                            <  3:243589/133‑1 (MQ=255)                                                                                                           aCCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGcc                         <  7:19258/135‑1 (MQ=255)                                                                                                            ccGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGGAGAAGACTTCACCGTCCAGGCTGGcc                         <  7:19263/134‑1 (MQ=255)                                                                                                                gccggccgGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGtt                    <  5:34358/135‑1 (MQ=255)                                                                                                                gccggccTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGtt                    <  8:92066/135‑1 (MQ=255)                                                                                                                  cggccGGGCGCGCCAGGGTCACGGCCGGGGCGTCCATAAGCGACCCAACCTGGAGTTCCACGCGCCGGGTGGCGGCCGGCGGCCGGGCAATGCGCGGCCGGGTGAACACTTCCACGTCCAGGGGGGCCTGGGtgg                  >  6:207347/1‑135 (MQ=255)                                                                                                                          cgcgcCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCGGGTTGGGTGGGGtg          <  4:152606/135‑2 (MQ=255)                                                                                                                            cgcCAGGGTCACGGCAGGGGCGTCCATCAGCGACACAACCTGCAGTTCCCCGCTCACGGTGGCGGCAGGCGGCAGGGCAAAGAGCTGCAGGTAGAAGACTTAACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTg        >  8:8769/1‑135 (MQ=255)                                                                                                                                tggggCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGGGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTg    <  1:192161/134‑1 (MQ=255)                                                                                                                                 gggTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCt     >  3:132180/1‑133 (MQ=255)                                                                                                                                 gggTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTgg   <  3:178268/135‑1 (MQ=255)                                                                                                                                  ggTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGa  <  6:283245/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     CAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGA  >  ACB122/1718345‑1718608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑