Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,718,490	T→G	18.7%	D56A (GAC→GCC)	CJ019_01614 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,718,490	0	T	G	18.7%	119.0 / 3.0	64	D56A (GAC→GCC)	CJ019_01614	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (10/40);  new base G (12/2);  total (22/42)
Fisher's exact test for biased strand distribution p-value = 1.21e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGAGCAGGCTGCAAGCA  >  ACB122/1718358‑1718622                                                                                                                                     |                                                                                                                                     aGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCGGGGGGCGGCAGGGGCGTcc                                                                                                                                    >  1:12390/1‑135 (MQ=255)       ctGCGCGCACTACCCGCATTGCGGTGGCCAGGCTTGCCCTGCGCGGGGTCATAGGGGAGTTGGAACGGCAGCGGCACGTTGCCTTGGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGc                                                                                                                              <  2:65935/134‑1 (MQ=255)       aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGGGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                                              <  7:296059/135‑1 (MQ=255)       aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                                              <  3:373363/135‑1 (MQ=255)       aTGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGc                                                                                                                              <  7:202106/135‑1 (MQ=255)          cgcgcACTTACCGCATAGCGGTGGGCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                                                           <  7:24075/135‑1 (MQ=255)          cgcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGGGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                                                           <  7:79312/135‑1 (MQ=255)          cgcgcACTTACCGCATAGCGGTGGCCAGGCGTGACCGGCGCGGGGGCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGac                                                                                                                           <  5:86/135‑1 (MQ=255)          cgcgaAATTACCGCATAGGGGGGGCCCGGCTTGACCGGGGGGGGGTCATCGGGGCGAGGCACCGGCCGCGGCACGTGGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGac                                                                                                                           <  4:23378/135‑1 (MQ=255)           gcgcACTTACCTCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTTGCGCGCCAGGGTCACCGCCGGGGCGTGCAGCcgcgcc                                                                                                                           >  6:267052/1‑132 (MQ=255)           gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGGGCGCCCGGGTCCCGGCAGGGGCGGCCAACAGCgccc                                                                                                                          >  6:22373/1‑132 (MQ=255)           gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCCGGGGCGGCCCTCAGCGcca                                                                                                                          >  4:153257/1‑135 (MQ=255)           gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCCCGGCCGGGGCGGCCCTCCGCGaca                                                                                                                          >  5:66975/1‑135 (MQ=255)            cgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGacac                                                                                                                         <  8:57311/135‑1 (MQ=255)               aCTTACCGATTAGCGGTGGCCAGGCTGGACGTGCGCGGGGTCATTGGTGACAGGGAACGGCAGCGGCAGGTTGCGTTTGACGGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATc                                                                                                                      <  4:327797/135‑1 (MQ=255)                cTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGGGACGGCCGGGGCGGGCCGCAGCGACCCCTcc                                                                                                                     >  8:16813/1‑135 (MQ=255)                     cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCCGGGGCGGCCCTCCGCGACCCCGCCTGCAg                                                                                                                >  8:202106/1‑135 (MQ=255)                            cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                                                          <  4:332367/134‑1 (MQ=255)                            cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCCCGGCCGGGGCGGCCCTCCCCGCCACATCCGTCCGTTccccc                                                                                                         >  2:346922/1‑132 (MQ=255)                            cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGGGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACg                                                                                                         <  2:281646/135‑1 (MQ=255)                                    aGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGGCCCGGCGGGGGCGGCCCTCAGCGCCCCATCCTGCGGGGCCGCGCGCAGGGt                                                                                                 >  3:325256/1‑135 (MQ=255)                                        ttGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGCCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                                                             <  7:130837/135‑1 (MQ=255)                                        gtgcccTGCGGGGGGGGATGGGTGAGCTGCACCGGACGCGCCACGTGGCGGTTGCCCGGGCGGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGGCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                                                             <  7:86637/131‑1 (MQ=255)                                        gtGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                                                             <  4:59614/134‑1 (MQ=255)                                         tGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCCGGGGCGTCCCTCAGCGCCACCTCCTGCAGTTCCCCGCGCCGGGGggcgg                                                                                            >  6:77998/1‑135 (MQ=255)                                         tGACCTGCGCGGGGTCATAGGTGAGATGCAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcgg                                                                                            <  5:267052/135‑1 (MQ=255)                                             gtGCGCGGGGTCTTAGGTGACATGTAACGCCAGCGGCAGGTTGCCTTTGACCGGGCCGTCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                                                        <  3:183853/134‑1 (MQ=255)                                             cTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                                                        <  8:265502/135‑1 (MQ=255)                                             cTGCGCGGGGGCATAGGTGAGATGGACCGGCCGCGGCACGTTGCCTGTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                                                        <  1:44959/135‑1 (MQ=255)                                              tGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCCGGGGCGCGGCCGGGGCGGCCCTCAGCGCCCCCGCCTGCAGGTCCCCCCTCCGGGGggcgggcggc                                                                                       >  5:187422/1‑130 (MQ=255)                                                   ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCAGGCGCGCCACGGGCACCGCCGGGGCGTCCATCCGCGACACCTCCAGCAGTTACACGCTCACGGGggcggccggcggccg                                                                                  >  3:107889/1‑133 (MQ=255)                                                       tCATAGGTTAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCa                                                                              <  3:129386/135‑1 (MQ=255)                                                        cATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGGGGGCGCGCCCGGGGCCCGGCCCGGGGGGGGCGCAGCGACACATCCTGCCGGTACACGCGGAGGGTGGCGGCCGGCGGCCGGGcca                                                                             >  8:244836/1‑133 (MQ=255)                                                         aTAGGTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAAt                                                                            <  3:184402/135‑1 (MQ=255)                                                             gggagaGGGAACGGCAGCGCCACGTTGCCTTTGCCCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                                                        <  2:187350/133‑1 (MQ=255)                                                             gTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGGGACCGCAGGGGCGGCCCTCAGCGCCACAACCAGCCCTTCCCCGCGCAGGGTGGCGGCCCGCGGCAGGGCCATgcgc                                                                        >  3:262796/1‑135 (MQ=255)                                                             gTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                                                        <  7:244836/135‑1 (MQ=255)                                                                   tGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCACGGCAGGGGCGGCCATCAGCGCCACAACCTGCACGTCCCCGCGCATGGTGGCGGCCGGCGGCCGGGCCATGCGCGGCAgg                                                                  >  6:249962/1‑135 (MQ=255)                                                                          gCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGGGCGGCCGGGGCCCGGCCGGGGGGGCCATCCGCGCCACAGCCGGCAGATCCCCGCGCCGGGGGGCGGGCGGCGGCCGGGCCATGCGCGGCAgggggcag                                                            >  8:59111/1‑128 (MQ=255)                                                                            cggggCAGGTTGCCTTTGCCGGGGCGGGCGGGGGGCGCCTGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                                         <  2:180218/132‑1 (MQ=255)                                                                            cggggCACGTGGCCTTTGACGGGGCCGGCGTGGTGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGTCAATGCGCTGCAGGTAGAAGACt                                                         <  1:158365/132‑1 (MQ=255)                                                                            cgCGGCAGGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                                         <  5:344722/134‑1 (MQ=255)                                                                            aGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                                         <  2:31027/135‑1 (MQ=255)                                                                            aGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                                         <  5:189187/135‑1 (MQ=255)                                                                            aGCGGCACGTTGCCGTTGACCGGGCCGGCCTGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                                                         <  4:223204/135‑1 (MQ=255)                                                                              cGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCACGGCAGGGGCGTCCATCAGCGCCCCAACCAGCAGTTCCACGCGCAGGGTGGCGGCCGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTc                                                       >  6:186783/1‑135 (MQ=255)                                                                                          tttGCCCGGGCCGGCTGGGCGCGCCAGGGGCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCt                                           <  5:372116/135‑1 (MQ=255)                                                                                             gACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTg                                          <  3:243589/133‑1 (MQ=255)                                                                                              aCCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGcc                                       <  7:19258/135‑1 (MQ=255)                                                                                               ccGGGCCGGCCGGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGGAGAAGACTTCACCGTCCAGGCTGGcc                                       <  7:19263/134‑1 (MQ=255)                                                                                                   gccggccgGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGtt                                  <  5:34358/135‑1 (MQ=255)                                                                                                   gccggccTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGtt                                  <  8:92066/135‑1 (MQ=255)                                                                                                     cggccGGGCGCGCCAGGGTCACGGCCGGGGCGTCCATAAGCGACCCAACCTGGAGTTCCACGCGCCGGGTGGCGGCCGGCGGCCGGGCAATGCGCGGCCGGGTGAACACTTCCACGTCCAGGGGGGCCTGGGtgg                                >  6:207347/1‑135 (MQ=255)                                                                                                             cgcgcCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCGGGTTGGGTGGGGtg                        <  4:152606/135‑2 (MQ=255)                                                                                                               cgcCAGGGTCACGGCAGGGGCGTCCATCAGCGACACAACCTGCAGTTCCCCGCTCACGGTGGCGGCAGGCGGCAGGGCAAAGAGCTGCAGGTAGAAGACTTAACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTg                      >  8:8769/1‑135 (MQ=255)                                                                                                                   tggggCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGGGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTg                  <  1:192161/134‑1 (MQ=255)                                                                                                                    gggTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCt                   >  3:132180/1‑133 (MQ=255)                                                                                                                    gggTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTgg                 <  3:178268/135‑1 (MQ=255)                                                                                                                     ggTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGa                <  6:283245/135‑1 (MQ=255)                                                                                                                         aCGGCAGGGGCGGCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGAGCAg            <  5:43713/135‑1 (MQ=255)                                                                                                                           ggCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGAGCAGGc          <  6:66975/135‑1 (MQ=255)                                                                                                                              aGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGAGCAGGCTGc       <  5:77998/135‑1 (MQ=255)                                                                                                                                   cGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGAGCAGGCTGCAAGCa  >  5:228837/1‑135 (MQ=255)                                                                                                                                   cGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGAGCAGGCTGCAAGCa  >  6:104355/1‑135 (MQ=255)                                                                                                                                     |                                                                                                                                     AGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCAGGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGGGGTTTGGCTGGAGCAGGCTGCAAGCA  >  ACB122/1718358‑1718622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑