Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,735,713	T→G	25.0%	D69A (GAC→GCC)	CJ019_01634 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,735,713	0	T	G	25.0%	104.7 / 2.5	72	D69A (GAC→GCC)	CJ019_01634	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/19);  new base G (3/18);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 2.13e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGTGCTGCTCGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGACGGGGGCATTACGCGCTGCAT  >  ACB122/1735579‑1735845                                                                                                                                       |                                                                                                                                     ccTGGTGCTGCTCGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCGACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGt                                                                                                                                      >  3:200705/1‑135 (MQ=255)   tGGTGCTGCTCGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCGACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCg                                                                                                                                    >  5:314788/1‑135 (MQ=255)   tGGTGCTGCTCGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCACCCATCGAGGCGATTGGGTCg                                                                                                                                    >  1:361620/1‑135 (MQ=255)       gctgctCGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGgggt                                                                                                                                >  3:333126/1‑135 (MQ=255)        ctgctCGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGgggtg                                                                                                                               >  7:49207/1‑135 (MQ=255)            tCGGCAGGGTAGACACCGGCAAGCGGCCCCAGCCAAACAATCGGCAGCCCAGTATCCGCGGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCACCCATCCCGGCTATTGGGTGGGggtgggtg                                                                                                                           <  8:274620/135‑1 (MQ=25)            tCGGCAGGCTAGACACCGGCCTGCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGAATATCTCTCTGCCTCTTCTTTGGTCCACCAGACGGCATTAACAACCATCCAGGCTATTGGGTCGGggtgggtg                                                                                                                           <  4:208924/135‑1 (MQ=25)            tCGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGCAGGCAGTACCAACCATCCAGGCTATTGGGTCGGggtgggtg                                                                                                                           >  1:230081/1‑135 (MQ=255)            tCGGCAGGCGAGACACCGGCCAGCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCGCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGggtgggtg                                                                                                                           <  4:25881/135‑1 (MQ=255)            tCGGCAGGCGACACACCGGCCACCGGCCCCAGCCCAACAATCGGCAGCCCTGTATCCGCCGCATCTCTCTCTGCGTCTTGTTTGGCCCACCAGACGCCAGAACCAACCATCCCGGCTATTGGGTGGGggtgggtg                                                                                                                           <  2:156168/135‑1 (MQ=255)             cGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGGCGGGgtgggtgg                                                                                                                          >  2:288396/1‑135 (MQ=255)             cGGCAGGCGAGAAACCGGCCATCGGCCCCAGCCCCACCATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTGTGGTCATCCAGAAGGCAGTAGCAACCATCCAGGCGATTGGGGCGGGgtgggtgg                                                                                                                          >  3:226782/1‑135 (MQ=255)              ggCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCCCCAGAAGGCAGTACCCACCATCCAGGCTATTGGGTCGGGGTGggtggt                                                                                                                         >  8:26654/1‑135 (MQ=255)              ggCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCCGGCTATTGGGTCGGGGTGggtggt                                                                                                                         >  4:118947/1‑135 (MQ=255)              ggCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGggtggt                                                                                                                         >  7:337397/1‑135 (MQ=255)                cAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCCGGCTATTGGGTCGGGGTGGGTGGTCg                                                                                                                       >  8:42608/1‑135 (MQ=255)                  ggCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCggg                                                                                                                     >  4:189574/1‑135 (MQ=255)                   gCGAGACACCGGCCAGCGGCCCCCGCCCAACACTCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTATTTGGACCACCACAAGGCCGTACCAACCATCCAGGCTATTGGGGCGGGGTGGGTGGTCgggg                                                                                                                    <  7:56231/135‑1 (MQ=37)                         cacCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCAGCTCTCTCCGCCTCTGCTTTGGTCCACCAGAAGGCAGAACCAACCATCCAGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCt                                                                                                              <  8:75066/135‑1 (MQ=38)                         cacCGGCCAGCGGCCCCAGCCCAACAAGCGGCCGCCCAGTATCCGCCGCATCTCTCTCTGCCGCTTCTTTGGGCCACCAGAAGGCAGTACCAACCATCCCGGCTATTGGGGCGGGGGGGGGGGTCGGGGCCAGCt                                                                                                              <  2:209708/135‑1 (MQ=37)                           ccGGCAAGCGGCCCCACCCCAACAATCGGCAGCCCAGTATCCGCCGCAGCTCTGTCTGCCTCTTCTTTGGTCCACCAGAAGCCAGTACCACCCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGc                                                                                                            <  4:121618/135‑1 (MQ=255)                              gCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGAATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGGCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTg                                                                                                         <  5:382284/135‑1 (MQ=255)                              cccATGGGCCCCAGCCCAAACCTCGCCAGCCATTTATCCGCCGCATATCTCGTTGCCAGTTGTTTGGCCCACCACAAGGCAGTAGCAAAAATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGGCAGCTGCGTg                                                                                                         <  8:249799/134‑1 (MQ=255)                                cATCGGCCCCAGCCCAACAATCGGCATCCAAGTATAAGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCCGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCa                                                                                                       >  8:283289/1‑135 (MQ=37)                                      ccccAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTg                                                                                                    >  8:345825/1‑132 (MQ=255)                                       cccAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCACCCATCCAGGCTATTGGGTCGGGGGGGGTGGTCGGGGCCAGCTGCGTGCACTg                                                                                                    >  4:111810/1‑131 (MQ=255)                                        ccAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATGGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGgaga                                                                                               <  1:318336/135‑1 (MQ=255)                                        ccAGCCCAACAATCGCCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCCGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGgaga                                                                                               <  8:60449/135‑1 (MQ=255)                                                          gCCCAGTAGCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGCAGGCTGTACCAACATTCCAGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAg                                                                             <  3:257310/135‑1 (MQ=255)                                                            ccAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGGCGGGGGGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCt                                                                           <  1:136250/135‑1 (MQ=255)                                                             cAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCgggg                                                                                                                    <  4:209276/93‑1 (MQ=255)                                                             cAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCgggg                                                                                                                    >  3:209276/1‑93 (MQ=255)                                                                       agcAGCTCTCTATGCCTCTTGTTGGGTCCACCAGATGGCTGTACCAACCATCCCGGCTTTTGGGTCGGGGTGGGGGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCaa                                                                <  2:358666/134‑1 (MQ=255)                                                                        gcATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCCACCATCCAGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAg                                                               >  7:107760/1‑135 (MQ=255)                                                                        gcATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAg                                                               >  3:295493/1‑135 (MQ=255)                                                                        gcATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAg                                                               >  4:152506/1‑135 (MQ=255)                                                                             tctctctGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGAGGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTg                                                          >  6:223490/1‑135 (MQ=255)                                                                                    gCCTCGTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCAGCGTGCACTGGGAGATGGCGGCATTGTAGCGAGCTGAAACCTCCACGTCCTGGCGAGCa                                                   >  4:258886/1‑135 (MQ=38)                                                                                        cttcttTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCCCTGGGAGAAGGCTGCTATGTAGCGAGCTGAAAACTCCAAGTCCTGGCGcgcacgca                                               >  5:274419/1‑135 (MQ=255)                                                                                          gcttTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATGGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCAtt                                             <  7:176325/134‑1 (MQ=255)                                                                                           cttTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCAt                                              >  5:344143/1‑133 (MQ=255)                                                                                            tttgtcccaccaGACGCCAGTACCAACCATCCAGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCAtt                                             <  3:258886/127‑1 (MQ=39)                                                                                            tttGGTCCACCTGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGGGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTg                                            <  7:372325/134‑1 (MQ=255)                                                                                            tttGGTCCACCAGAAGGCAGTACCAACCTTCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTg                                            <  2:96971/134‑1 (MQ=255)                                                                                            tttGGTCCACCAGAAGGCAGTACCAACCATCCCGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTg                                            <  8:320651/134‑1 (MQ=255)                                                                                            tttGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCAtt                                             <  2:369703/133‑1 (MQ=255)                                                                                            tttGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCAtt                                             <  5:88373/133‑1 (MQ=255)                                                                                            tttGGGCCACCAGAAGGCATTACCAACCATCCAGGGTATGGGGTCGGGGGGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTg                                            <  2:361620/134‑1 (MQ=255)                                                                                            tttGGGCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGGCGGGGGGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTg                                            <  5:3047/134‑1 (MQ=255)                                                                                            tttGGGCCACCAGAAGGAAGTACCAACCATCCAGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTg                                            <  8:232554/134‑1 (MQ=255)                                                                                             ttGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAg                                          >  5:167265/1‑135 (MQ=255)                                                                                              tGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGt                                         >  3:117030/1‑135 (MQ=255)                                                                                                 gccaccaGACGCCAGTACCACCCATCCAGGCTATTGGGGCGGGGGGGGGGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCg                                       <  2:230081/133‑1 (MQ=255)                                                                                                  ccacctGAAGGCTGTACCAACCTTCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGAc                                     <  4:200705/135‑1 (MQ=255)                                                                                                  ccaccaGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGAc                                     <  4:333126/135‑1 (MQ=255)                                                                                                   caccaGAAGGCAGTACCAACCATCCCGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCAGTGAGTCGAc                                     >  2:316086/1‑134 (MQ=255)                                                                                                    accccaaGGCAGTACCAACCTTCCTGGCTATTGGGGGGGGGTGGGTGGTCGGGGCCAGCTGCGTGCTCTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTGGACAt                                   <  7:340273/130‑1 (MQ=255)                                                                                                    accaGAAGGCAGTACCAACCATCCCGGCTATTGGGTCGGGGGGGGGGGGCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACAt                                   <  7:121313/135‑1 (MQ=255)                                                                                                      caGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATac                                 >  5:91570/1‑135 (MQ=255)                                                                                                             cAGAACCAACCATCCAGGCTTTTGGGGGGGGGGGGGGGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCg                          <  3:189574/135‑1 (MQ=255)                                                                                                                tACCAACCATCCAGGCTATTGGGGCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGa                       <  8:337397/135‑1 (MQ=255)                                                                                                                 accaaccaTCCCGGCTATTGGGGGGGGGTGGGGGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGa                       <  7:283289/134‑1 (MQ=255)                                                                                                                          cctggCTATTGGGTCGGGGGGGGTGGTCGGGGCCAGCTGCGTGCACTGTGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGACGGGGGCAtt             <  3:45580/132‑1 (MQ=255)                                                                                                                          ccAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGACGGGGGCAtt             >  5:269542/1‑135 (MQ=255)                                                                                                                            cgggtATTGGGTGGGGGGGGGGGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGACGGGGGCATTAc           <  5:346517/131‑1 (MQ=255)                                                                                                                             ggCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTTTAGCGAGCTGAAACCTCCAAGTCCTGGCGAGCACGCATTGAGTCGCCATACACCCTCGGGACGGGGGCATTAcg          >  5:280588/1‑135 (MQ=255)                                                                                                                             ggCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTg                                            <  4:117558/101‑1 (MQ=255)                                                                                                                             ggCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTg                                            >  3:117558/1‑101 (MQ=255)                                                                                                                             ggCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGACGGGGGCATTAcg          >  4:53560/1‑135 (MQ=255)                                                                                                                             ggCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGACGGGGGCATTAcg          >  7:350452/1‑135 (MQ=255)                                                                                                                             ggCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACAACATCGGGACGGGGGCATTAcg          >  6:214603/1‑135 (MQ=255)                                                                                                                                 aTTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGACGGGGGCATTACGCGCt      >  8:116403/1‑135 (MQ=255)                                                                                                                                     ggTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACAACATCGGGACGGGGGCATTACGCGCTGCAt  >  5:195971/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                     CCTGGTGCTGCTCGGCAGGCGAGACACCGGCCATCGGCCCCAGCCCAACAATCGGCAGCCCAGTATCCGCCGCATCTCTCTCTGCCTCTTCTTTGGTCCACCAGAAGGCAGTACCAACCATCCAGGCTATTGGGTCGGGGTGGGTGGTCGGGGCCAGCTGCGTGCACTGGGAGATGGCTGCTTTGTAGCGAGCTGAAAACTCCAAGTCCTGGCGAGCACGCATTGAGTCGACATACACCATCGGGACGGGGGCATTACGCGCTGCAT  >  ACB122/1735579‑1735845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑