| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,779,174 | G→A | 9.3% | intergenic (+134/‑52) | rpsB → / → tsf | 30S ribosomal protein S2/Elongation factor Ts |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,779,174 | 0 | G | A | 9.3% | 124.6 / 4.2 | 43 | intergenic (+134/‑52) | rpsB/tsf | 30S ribosomal protein S2/Elongation factor Ts |
| Reads supporting (aligned to +/- strand): ref base G (19/20); new base A (2/2); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
GCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCC‑TTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGCGAAGGCATGATGGATTGCAAAAAG > ACB122/1779046‑1779300 | gcTAGCGTCTACTTGGCACGCAAAAAGGGGGGTTTTCCCCCGTTTTTGCC‑CCTTTTAATCCAGCTGGAAGCAACCGCCCCCCATGATGGGGCAGCTGAAATGAAAACAGCGGATTTTGAGAATTTCACGccccgg > 6:127773/1‑132 (MQ=255)gcTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCC‑TTTTTGGCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCGGAAATGAAAACAGCGGATTTGCAGCATTGCACGCCCggg > 1:62689/1‑133 (MQ=255) ctcgCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCC‑TTTTTGCCACCTTGAAATCCTGCTGGCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGa < 1:258881/132‑1 (MQ=255) aGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGACCCC‑TTTTTGCCACCTTTAAATCCTGCTGTCAGCATAGGCCCCGCATCATGGGCCTGCTGAAATGAAAAAACCGGATTTGCAGCATTGAACACCCGTGAt > 7:207512/1‑134 (MQ=255) cgccTACTTGGCACGCAAAAAGGGGGCGTTGCCCCT‑TTTTTGCCACCTTGAAATCCTGCTGTCAGCTTCGGCCCCGCATCTTGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGAtt < 2:266845/130‑1 (MQ=255) cGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCC‑GTTTTGCCCCCTTGAAATCCTGCTGTCAGCATAGGCCCCGCCTCATGGGCCGGCGGCAATGAAAACAGCGGAGTTGCAGAATTGAACGCCCGTGATTGa > 7:194578/1‑135 (MQ=255) cGTCTACTTGGCACGCAAAAAGGGGGCGTTGCCCCC‑TTTTTGCCACCTTGAAATCCAGCTGTCAGCATCCGCCCCGCCTCATGGGCCTGCGGAAATGTAAACCGCGGATTTGCAGCATTGAACGCCCGTGaatta > 8:212561/1‑131 (MQ=255) ttGGCACGCAAAAAGGGGGCGTTGGCCCC‑GTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCAGGGGCCTGCGGAAATGAAAACAGCGGATTTGGCGAATTGTACGCCCGTGCTTGCCggggg > 8:195934/1‑132 (MQ=255) aaaaGGGGGCTTTGCCCCC‑TTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGaa < 2:254921/135‑1 (MQ=255) cTTTGCCCCC‑TTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTcc < 3:227982/135‑1 (MQ=255) tttGCCCCC‑TTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGGGATTGACGGGTGGAATGGTTGAAAAACTTTc > 6:380383/1‑133 (MQ=255) tGCCCCC‑TTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCa > 2:205414/1‑133 (MQ=255) cccc‑TTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAgg > 4:307383/1‑135 (MQ=255) ccTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGgc < 8:229493/135‑1 (MQ=255) ttGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGgca > 3:248136/1‑134 (MQ=255) gAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGgcagca > 4:330470/1‑135 (MQ=255) ctgTCAGCAGCGGCCCCGCATCATGGGCCGGCTGAAATGAAAACAGCGGATTTGAAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAAATTTCCAAGAGGAGTTTGAAATGGCAGCAATTACTGCa < 6:177776/135‑1 (MQ=255) ctgGCAGCATCGGCCCGGCATCATGGCCCTGCTGAAATGAAAACAGCGGATTTGCATATTTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCa < 5:12786/135‑1 (MQ=255) tgTCAGCATCGGCCCCGCCTCATGGGCCTGCTGAAATGAAAACCGCGGTTTTGCAGAATTGAACGCCCGTGATTGACGGGGGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCa < 7:352205/134‑1 (MQ=255) tgTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCa < 3:244263/134‑1 (MQ=255) tgTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCa < 5:339931/134‑1 (MQ=255) tgTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCa < 7:295446/134‑1 (MQ=255) tgTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAg > 8:288993/1‑135 (MQ=255) tgTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACACCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCa < 2:290227/134‑1 (MQ=255) tgTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACACCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAg > 1:290227/1‑135 (MQ=255) tCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCa < 2:168006/132‑1 (MQ=255) tCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCa > 1:168006/1‑132 (MQ=255) cAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCTATTACTGCAgc < 8:314110/133‑1 (MQ=255) cAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAgc < 7:13745/133‑1 (MQ=255) aaGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAgc < 4:225366/132‑1 (MQ=255) tgaaatgaaaACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCgg > 6:47827/1‑135 (MQ=255) aaatgaaaACAGCGGATTTGCAGAATTGAACGCCCGTGAtt < 7:139506/41‑1 (MQ=255) aaatgaaaACAGCGGATTTGCAGAATTGAACGCCCGTGAtt > 8:139506/1‑41 (MQ=255) aaatgaaaACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGc > 5:305694/1‑134 (MQ=255) aaatgaaaACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGc > 1:189021/1‑134 (MQ=255) aatgaaaACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGc < 8:194578/133‑1 (MQ=255) aaaaCAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGCGAAGGc > 6:182362/1‑135 (MQ=255) aaaaAGGGGATTTGCAGCAATGAACGCCCGTGATTGAAGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGCGAAGGc < 2:334477/134‑1 (MQ=255) aaaCAGCGGATTTGCAGAATTGAACACCCGTGATTGACTGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGCGAAGGc < 8:207512/134‑1 (MQ=255) gCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGCGAAGGCatgatg < 7:288993/135‑1 (MQ=255) aTTTGCAGAATTGAACGCCCGTGATTGACGGTTGGAATGGTTGAAAAACTTTCCAAGAGGATATTGAAATGGCAGCAATTACTGCAGCGCTCGTAAAAGAACTGAGCGAGCGTACCGGCGAAGGCATGATGGAt > 7:60212/1‑134 (MQ=255) aTTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGCGAAGGCATGATGGAtt > 5:128141/1‑135 (MQ=255) aaTTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGCGAAGGCATGATGGATTGCAAAAAg < 7:212561/135‑1 (MQ=255) | GCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCC‑TTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAGGATTTTGAAATGGCAGCAATTACTGCAGCGCTGGTAAAAGAACTGCGCGAGCGTACCGGCGAAGGCATGATGGATTGCAAAAAG > ACB122/1779046‑1779300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |