| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 1,787,510 | T→A | 6.5% | F558I (TTC→ATC) | bamA_1 → | Outer membrane protein assembly factor BamA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,787,510 | 0 | T | A | 6.5% | 195.8 / 4.0 | 62 | F558I (TTC→ATC) | bamA_1 | Outer membrane protein assembly factor BamA |
| Reads supporting (aligned to +/- strand): ref base T (24/34); new base A (2/2); total (26/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
CGTCAGCCTGGGCTACCCGATCAGCGAAACCTCGCGCCTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGCGACCTGTCGTT > ACB122/1787377‑1787634 | cGTCAGCCTGGGCTACCCGATCAGCGAAACCTCGCGCCTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACtt < 6:22414/135‑1 (MQ=255)cGTCAGCCTGGGCTACCCGATCAGCGAAACCTCGCGCCTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACtt < 4:297665/135‑1 (MQ=255) cTGGGCTACCCGATCAGCGAAACCTCGCGCCTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAAc > 7:311163/1‑135 (MQ=255) cTGGGCTACCCGATCAGCGAAACCTCGCGCCTGACCTAAGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCGCGAGGACGAAGGTGACAACTTCCTGAAc > 2:378931/1‑135 (MQ=255) tGGGCTACCCGATCAGCGAAACCTCGCGCCTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCATATACACTGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACt < 1:352701/135‑1 (MQ=255) ccGATCAGCGAAACCTCGCGCCTGACCTACGGCCTGAGCGTGCAGCAGTACAACATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAgg < 1:186421/133‑1 (MQ=255) gATCAGCGAAACCTCGCGCCTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCt > 7:84822/1‑134 (MQ=255) cgcgCCTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACGGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCg < 5:316446/135‑1 (MQ=255) cgcCTGACCTACGGCCGGCGCGCGCAGCAGGACAAGCTCAAGACCTGCAAAAACACCGTTGACGAGAGCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGAGCGGCTGGTCCg < 6:365010/133‑1 (MQ=255) cgcCTGACCTACGCCCTCAGCGTGCAGAAGGACAAGAGCACGACCGGCAAATAAACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAATGCCGCGATCGGCTGGTCCg < 3:347478/133‑1 (MQ=255) cTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCg < 6:69323/135‑1 (MQ=255) cTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCg < 5:243233/135‑1 (MQ=255) cTGACCTACGGCCTGAGCGTGCAGCAGCACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCg < 3:185674/135‑1 (MQ=255) cTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCt < 8:72032/135‑1 (MQ=255) cTACGGCCTGAGCGTGCAGCAGGACAAGAGCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCt < 7:81591/135‑1 (MQ=255) tACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCTAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCt > 6:102857/1‑134 (MQ=255) gCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACa < 3:263201/135‑1 (MQ=255) gCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACa < 4:140738/135‑1 (MQ=255) gCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGc < 8:263422/134‑1 (MQ=255) gTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTa > 5:368034/1‑135 (MQ=255) gTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTa > 7:47330/1‑135 (MQ=255) gTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTa > 7:265813/1‑135 (MQ=255) gTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCATGAACAAAGGCGTa > 6:305217/1‑135 (MQ=255) gcagcaGGACAAGATCAAGACCGGCTAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACt < 5:102857/135‑1 (MQ=255) cagGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCGGAACAAAGGCGTACTGGcc > 2:286501/1‑135 (MQ=255) aaGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGt > 5:175687/1‑135 (MQ=255) aaGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGt < 5:184993/135‑1 (MQ=255) gATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTgg < 2:87284/135‑1 (MQ=255) gATCAAGACCGGCAAATACACCGTTGACGAGATCTTAGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTgg < 2:113355/135‑1 (MQ=255) cAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCa > 2:8188/1‑135 (MQ=255) aaGACCTGCAAATCCTCCGTTGACGAGATCTTGGACTTCCTCGAGGAGGAAGGTGACATCTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAAGAAAGGCGTACTGGCCACCCGTGGTCAc < 1:372947/135‑1 (MQ=38) aGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACt > 7:290516/1‑135 (MQ=255) ccGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACACAGGCGTACTGGCCACCAGTGGTCACTCg > 6:276588/1‑134 (MQ=255) ccGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCg > 8:223210/1‑134 (MQ=255) cGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGc > 6:17401/1‑134 (MQ=255) gCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCaaa < 7:104266/135‑1 (MQ=255) gCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCaaa < 8:57328/135‑1 (MQ=255) gCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCaaa < 2:350198/135‑1 (MQ=255) gCAAATACACCGTTGACGAGAGCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGGCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCaaa < 1:133523/135‑1 (MQ=255) cAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAg < 8:84822/135‑1 (MQ=255) aaTACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGcc > 8:219966/1‑135 (MQ=255) aaTACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGcc > 7:286002/1‑135 (MQ=255) aaTACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCAGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGcc > 8:176252/1‑135 (MQ=255) aaTACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACATCCTGAAc > 2:246486/1‑59 (MQ=255) aaTACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACATCCTGAAc < 1:241000/59‑1 (MQ=255) aaTACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACATCCTGAAc < 1:246486/59‑1 (MQ=255) aaTACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACATCCTGAAc > 2:241000/1‑59 (MQ=255) taaccggTTGAGGAGAGCTTCGACTTCCTCGAGTAGGAAGGTGACAACTTACTGAACTTCAAGTCCTCGATGGTGTGGTCCGAGTAGACCCTGAACATAGGCGTACTGGCCACCCGTGGTCACTCGCATAGCCTg < 7:360158/129‑1 (MQ=255) gACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTTGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAg > 8:141838/1‑135 (MQ=255) ctagaTCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGt < 2:176275/132‑1 (MQ=255) aTCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCATCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAACCCTGACCCTGGAGTCCAcc > 1:170417/1‑135 (MQ=255) ttCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCggt > 5:216960/1‑133 (MQ=255) ttCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGtt > 6:286250/1‑135 (MQ=255) gACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTcc < 5:286250/134‑1 (MQ=255) aCTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCgg < 4:360947/135‑1 (MQ=255) aCTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGAGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCgg < 6:44580/135‑1 (MQ=255) aCTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTAAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGAGGTCACTCTCAAAGCCTGACCCTGGAGTCCACCGTTCCgg > 2:268376/1‑135 (MQ=255) tCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCGTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGc < 1:121219/134‑1 (MQ=255) cGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGCGa > 6:382103/1‑135 (MQ=255) gaggaAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGCGACCTg < 6:175687/135‑1 (MQ=255) gaggaAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGCGACCTg < 8:286002/135‑1 (MQ=255) gaggaAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGCGACCTg < 8:128258/135‑1 (MQ=255) gaggaAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGCGACCTg < 2:99377/135‑1 (MQ=255) aGGTGACAACTTCCTGAACTTCAAGGCCTTGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGCGACCTGTCGtt < 7:141838/135‑1 (MQ=255) | CGTCAGCCTGGGCTACCCGATCAGCGAAACCTCGCGCCTGACCTACGGCCTGAGCGTGCAGCAGGACAAGATCAAGACCGGCAAATACACCGTTGACGAGATCTTCGACTTCCTCGAGGAGGAAGGTGACAACTTCCTGAACTTCAAGGCCTCGATCGGCTGGTCCGAGTCGACCCTGAACAAAGGCGTACTGGCCACCCGTGGTCACTCGCAAAGCCTGACCCTGGAGTCCACCGTTCCGGGCAGCGACCTGTCGTT > ACB122/1787377‑1787634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |