Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	113,311	T→C	6.4%	E27E (GAA→GAG)	CJ019_00120 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	113,311	0	T	C	6.4%	192.9 / 3.3	62	E27E (GAA→GAG)	CJ019_00120	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/25);  new base C (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

GTGCATCATCCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGTGCGCA  >  ACB122/113187‑113445                                                                                                                             |                                                                                                                                       gTGCATCATCCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGgcg                                                                                                                              >  1:190794/1‑135 (MQ=255) gTGCATCATCCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGgc                                                                                                                               >  1:127604/1‑134 (MQ=255) gTGCATCATCCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGgc                                                                                                                               >  5:81608/1‑134 (MQ=255) gTGCATCATCCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGgc                                                                                                                               >  3:247292/1‑134 (MQ=255)     atcatcCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCAtt                                                                                                                          >  2:35671/1‑135 (MQ=255)      tcatcCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCAtt                                                                                                                          >  8:251521/1‑134 (MQ=255)      tcatcCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTg                                                                                                                         >  2:378393/1‑135 (MQ=255)          ccGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAAc                                                                                                                     <  8:316494/135‑1 (MQ=255)          ccGGCGACGGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAAc                                                                                                                     <  6:121080/135‑1 (MQ=255)            ggCGACTGGGCCTGCAGAGCCGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAg                                                                                                                   >  8:6040/1‑135 (MQ=255)                     gCCTGCAGAGCCGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAg                                                                                                           <  7:6040/134‑1 (MQ=255)                       cTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGa                                                                                                         >  7:18266/1‑134 (MQ=255)                               cTGCAAGCTGGTCTTCGGCGGGAGCCAGCTCGGTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGaaa                                                                                                <  4:255789/135‑1 (MQ=255)                               cTGCAAGCGGGTCTTCGGCGGTAGCCAGCTCGTTGCTGTCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGTAACAGGCAGCCAGGATCAGGGata                                                                                                <  1:278224/135‑3 (MQ=255)                                  cAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAAcg                                                                                             >  3:349070/1‑135 (MQ=255)                                  cAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAAcg                                                                                             >  7:114358/1‑135 (MQ=255)                                     gCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTTGCGATGTTGTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCACGTGCGGTGGCCTGTTCGACCCGCGCATTGGAACACGCAGCCAGTATCAGGGAAAAagcg                                                                                           >  6:284477/1‑134 (MQ=255)                                     gCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGCGAAAAcgcg                                                                                           >  8:348622/1‑134 (MQ=255)                                          tCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGcaac                                                                                      <  7:251521/134‑1 (MQ=255)                                            ttCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGcaacaa                                                                                    >  4:272468/1‑134 (MQ=255)                                            ttCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGcaacaa                                                                                    >  8:210948/1‑134 (MQ=255)                                            ttCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAg                                                                                   <  2:190794/135‑1 (MQ=255)                                            ttCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGCTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGcaacaa                                                                                    >  4:220497/1‑134 (MQ=255)                                            ttCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGCTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGcaacaa                                                                                    >  4:224888/1‑134 (MQ=255)                                                 cggTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGa                                                                               >  6:71528/1‑134 (MQ=255)                                                  ggTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACCGCGGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATa                                                                             >  7:176945/1‑135 (MQ=255)                                                       ccAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACg                                                                          >  3:292775/1‑133 (MQ=255)                                                           cTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGc                                                                    <  3:272468/135‑1 (MQ=255)                                                              gTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGaa                                                                 >  2:114558/1‑135 (MQ=255)                                                                   tGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCt                                                            >  7:15705/1‑135 (MQ=255)                                                                   tGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCt                                                            >  8:371217/1‑135 (MQ=255)                                                                    ggCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCtt                                                           <  7:282878/135‑1 (MQ=255)                                                                         tGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTc                                                      >  7:293546/1‑135 (MQ=255)                                                                          gTTCTGTTGCAACTGCTGCCAGGCAGCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTcc                                                     <  2:21978/135‑1 (MQ=255)                                                                            tCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGTCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTg                                                   <  7:354585/135‑1 (MQ=255)                                                                            tCTGTTGCAACCGCGGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTg                                                   <  8:176945/135‑1 (MQ=255)                                                                              tggtccAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACCGGCAGCCAGGATCAGGTAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCg                                                 <  3:268717/130‑1 (MQ=255)                                                                              tGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCg                                                 >  1:372905/1‑135 (MQ=255)                                                                                   cAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGActct                                            >  2:369912/1‑135 (MQ=255)                                                                                       tgctgcCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAACCGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTAt                                        <  6:56428/135‑1 (MQ=255)                                                                                        gctgcCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGc                                                             <  4:278956/113‑1 (MQ=255)                                                                                        gctgcCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGc                                                             >  3:278956/1‑113 (MQ=255)                                                                                        gctgcCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTAtt                                       >  6:89602/1‑135 (MQ=255)                                                                                        gatgcCAGGCATCTGCTTGAGCAGGTGCGGTGGACTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTAtt                                       >  6:241147/3‑135 (MQ=255)                                                                                                    cTGCTTGAGCAGGTGCGGTGGCCTGCTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGc                           <  3:220497/135‑1 (MQ=255)                                                                                                     tGCTTGTGCAGGTGCGGTGGCGTGTTCGACCGGCGCATTGTAACAGGCAGCCAGGATCAGGTAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGc                           <  7:348622/134‑1 (MQ=255)                                                                                                     tGCTTGAGCAGGTGCGGTGGCCTGCTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGc                           <  3:224888/134‑1 (MQ=255)                                                                                                        ttGAGGAGGTGCGGTGGCCTGTTCGACGGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGACGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGc                           <  2:253556/131‑1 (MQ=255)                                                                                                        ttGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGc                           <  4:102800/131‑1 (MQ=255)                                                                                                        ttGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCg                          <  7:371217/132‑1 (MQ=255)                                                                                                        ttGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAg                        >  2:169207/1‑134 (MQ=255)                                                                                                        ttGAGCAGGTGCGGGGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCg                          <  6:304367/132‑1 (MQ=255)                                                                                                           aGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCa                      <  8:18266/133‑1 (MQ=255)                                                                                                            gCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAg                    <  1:299103/134‑1 (MQ=255)                                                                                                            gCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGt                   <  8:114358/135‑1 (MQ=255)                                                                                                            gCAGGTGCGGTGGCCTGTGCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAg                    <  4:247292/134‑1 (MQ=255)                                                                                                              aGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCt                 >  6:322299/1‑135 (MQ=255)                                                                                                                   cGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGCCTCTCTATTTGCGCAAACAGCGAGCAAGTCTACAcc            >  6:9470/1‑135 (MQ=255)                                                                                                                    ggTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTacac             >  2:293838/1‑133 (MQ=255)                                                                                                                    ggTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTacac             >  6:199624/1‑133 (MQ=255)                                                                                                                        gCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGt       >  7:184206/1‑135 (MQ=255)                                                                                                                          cTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGTgc     <  1:114558/135‑1 (MQ=255)                                                                                                                             ttCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTac               <  8:111700/122‑1 (MQ=255)                                                                                                                             ttCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTac               >  7:111700/1‑122 (MQ=255)                                                                                                                             ttCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGTgc     >  8:251168/1‑132 (MQ=255)                                                                                                                             ttCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGTgc     >  2:6396/1‑132 (MQ=255)                                                                                                                             ttCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGTgc     <  1:6396/132‑1 (MQ=255)                                                                                                                             ttCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGTgc     <  7:251168/132‑1 (MQ=255)                                                                                                                             ttCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGTGCGCa  >  8:47951/1‑135 (MQ=255)                                                                                                                             |                                                                                                                                       GTGCATCATCCGGCGACTGGGCCTGCAGAGCTGCAAGCTGGTCTTCGGCGGTAGCCAGCTCGTTGCTGGCGATGTTCTGTTGCAACTGCTGCCAGGCATCTGCTTGAGCAGGTGCGGTGGCCTGTTCGACCGGCGCATTGGAACAGGCAGCCAGGATCAGGGAAAACGCGGCAACAAGCAGATAACGGGAGGCGAACGGCTTCATTCCTGCGACTCTCTATTTGCGCAAAAAGCGAGCAAGTCTACACCCAGGTGCGCA  >  ACB122/113187‑113445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑