Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,791,678 T→G 22.1% L182R (CTG→CGG)  lpxB → Lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,791,6780TG22.1% 62.2 / 5.6 48L182R (CTG→CGG) lpxBLipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  ref base T (29/6);  new base G (3/8);  total (34/14)
Fisher's exact test for biased strand distribution p-value = 1.19e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTG  >  ACB122/1791560‑1791811
                                                                                                                       |                                                                                                                                     
ctgctgCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGGCCCGCTGGGCCGGGGCGAgggg                                                                                                                       >  5:10737/1‑135 (MQ=255)
                           gAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCCCGCCTGCCGCGCCTGGCCAGGGCGCCGGGCCCGGGGTGGGGGTGCTGGCGGGCCGc                                                                                            >  4:1443/1‑135 (MQ=255)
                               agCAGGGTGTGCCCGTGCGTTTTGCCGGCCACCC‑GCTGGCAGACACCATACCGCTTGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCggg                                                                                        <  2:86843/135‑1 (MQ=255)
                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGCCGGGGTg                                                                                     >  1:21115/1‑135 (MQ=255)
                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGCTGCCGGGCAGCCGGGGTg                                                                                     >  4:58061/1‑135 (MQ=255)
                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  1:33031/1‑135 (MQ=255)
                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  6:198467/1‑135 (MQ=255)
                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  4:147140/1‑135 (MQ=255)
                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTg                                                                                     >  3:96839/1‑135 (MQ=255)
                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTAGATGCCGGGCAGCCGGGGTg                                                                                     >  3:226589/1‑135 (MQ=255)
                                  aGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGATGCCGGGCCGCCGGGGTg                                                                                     >  4:116420/1‑135 (MQ=255)
                                       gtgCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGCGGGGCCGGTGGTGGCGGTGCTTCCCGGCAGCCGGGGTGGCgca                                                                                >  3:313407/1‑133 (MQ=255)
                                         gCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGt                                                                              <  2:33031/135‑1 (MQ=255)
                                            aGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGCAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTGGCTGCCGGGCCGCCGGGGTGGCGAAGTAgg                                                                           >  5:73858/1‑135 (MQ=255)
                                            aGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCCGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  3:231906/1‑135 (MQ=255)
                                            aGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCAGGGCGAGGGGCCCGAGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAgg                                                                           >  4:353413/1‑135 (MQ=255)
                                                 gTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCt                                                                      >  3:339080/1‑135 (MQ=255)
                                                 gTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCt                                                                      >  7:228904/1‑135 (MQ=255)
                                                 gTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCAGGGCGCGGGGCCCGTGGTGGCGGTGCTGCCGGGCAGCCCGGGTGGCGAAGTAGGGCGCt                                                                      >  5:294164/1‑135 (MQ=255)
                                                 gTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGGGGGGGGCCGGGGGGGGGGGTGGTGCCGGGCCGCCGGGGGGGGGGAGGTGGgcgcg                                                                      >  5:216433/1‑134 (MQ=255)
                                                 gTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCCCGCAGGGGCGTGGGGCGGGGGCGGGGGGGGCGGTGATGGCGGGGCGCAGGGGGGGGGAATTATGgggcg                                                                      >  2:158599/1‑134 (MQ=255)
                                                     tggcGGCCACCC‑GCTGGCAGACACCATACCGCTTGCAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTgg                                                                   <  4:73905/131‑1 (MQ=255)
                                                     tGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCCCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGACGTCGGGCGCATggg                                                                  >  7:194893/1‑135 (MQ=255)
                                                       tCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 <  8:228904/134‑1 (MQ=255)
                                                       tCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGGGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgcg                                                                <  7:372337/135‑1 (MQ=255)
                                                       tCGGCCACCC‑GCTGGAATACACCATCCGGCTTGACGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 <  6:313845/134‑1 (MQ=255)
                                                       gcGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgc                                                                 <  8:116629/133‑1 (MQ=255)
                                                       ccGGCCCCCC‑GCGGGCACACACCTCCCCGCTTGACGCGGCCCGCCCGCCCGCGCGGGCGGCGCGGGGCCGGGGGGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgcg                                                                <  7:160901/134‑1 (MQ=255)
                                                        cGGCCCCCCGGCTGGCCGACACCATCCCGCTGGACGCCCCCCCCCGGGCGGGGCGGGCCGCGCGGGCCGGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGgcg                                                                <  3:215536/135‑1 (MQ=255)
                                                        cGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGGGTGGCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGGTGATGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCTTGGgcgc                                                               >  5:370959/1‑135 (MQ=255)
                                                        cGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGTAGTAGGGCGCGTGGgcgc                                                               >  4:323517/1‑135 (MQ=255)
                                                                   gccccAGACCCCATACCGGTTGACGCCGCCCGCCCGGCCGCGCGGGCGGCGCGGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCt                                                      <  2:250485/130‑1 (MQ=255)
                                                                           ccccATCCCGCGGGACGCCGCCCGCCCGGCCGCGCGGGCCGCGCGGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGAcgccgc                                             <  6:216433/133‑1 (MQ=255)
                                                                                 aCCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAgcg                                       >  8:291509/1‑135 (MQ=255)
                                                                                   cGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGcc                                     >  2:269078/1‑135 (MQ=255)
                                                                                         acgCCGACCGCCCGGCCGCGCGGGCCGCGCTGGGCCGGGGCGAGGGGCCGGTGGGGGCGTTGATGCCGGGGACCCGGGGGGGCGAAGTAGGGCGCTTTGGCCCGCTGTTCCTCGCCGCCGACGCGCGCCTCAGca                               >  2:362708/3‑134 (MQ=255)
                                                                                         aaGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTTGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGcc                               >  8:355885/1‑135 (MQ=255)
                                                                                         aaGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCCGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                >  4:324942/1‑134 (MQ=255)
                                                                                         aaGCCGACCGCCCGGCCGCGCGGGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGc                                <  8:25396/134‑1 (MQ=255)
                                                                                          aGCCGCCCGCCCGGCCGCGCGGGCCGGGCGGGGCCGGGGCGCGGGGCCGGTGGTGGCGTTGATGCCGGGCAGTCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              <  3:149872/135‑1 (MQ=255)
                                                                                           gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGGCGGGGCGGGGGGCCCGTGGTGGCGGTGATGCCGGGCAGCCGGGGTGGGGCAGTAGGGCCCGTGGGCGCGCAGTTCCTCGACCCCCCCGAGCGCCtacccca                              >  2:72480/1‑128 (MQ=255)
                                                                                           gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCca                              >  7:356821/1‑134 (MQ=255)
                                                                                           gCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGGTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGCGCGCCTCAGCca                              >  4:49770/1‑134 (MQ=255)
                                                                                               aCCGCCCGGCGGCGCGTGCGGCGCTGGGCCGGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAg                         <  8:132068/135‑1 (MQ=255)
                                                                                                  gCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGc                      >  8:361271/1‑135 (MQ=255)
                                                                                                  gCCCGGCCGCGCGTGCCGCGCGGGGCCTGGGCGAGGGGCCGGTGGTGGGGGTGCTGGCGGGCAGCCGGGGGGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCCGCGCCGCCGCGCGCCGCCGCCCACAAGTGc                      >  1:367631/1‑135 (MQ=255)
                                                                                                                    cgcTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCgtgt    >  7:686/1‑135 (MQ=255)
                                                                                                                     gcTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGCCGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGtt   >  6:308337/1‑135 (MQ=255)
                                                                                                                      gtgggcggggggGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTg  <  5:292234/134‑1 (MQ=255)
                                                                                                                       |                                                                                                                                     
CTGCTGCCGTTCGAGGCGCGGTTCTACGAAGAGCAGGGTGTGCCAGTGCGTTTTGTCGGCCACCC‑GCTGGCAGACACCATACCGCTTGAAGCCGACCGCCCGGCCGCGCGTGCCGCGCTGGGCCTGGGCGAGGGGCCGGTGGTGGCGTTGATGCCGGGCAGCCGGGGTGGCGAAGTAGGGCGCTTGGGCGCGCTGTTCCTCGACGCCGCCGAGCGCCTCAGCCAACAAGTGCCGGGTGTGCGCTTCGTGTTG  >  ACB122/1791560‑1791811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑