Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,806,746 G→C 100% W15C (TGG→TGC)  yeiG → S‑formylglutathione hydrolase YeiG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,806,7460GC100.0% 262.1 / NA 76W15C (TGG→TGC) yeiGS‑formylglutathione hydrolase YeiG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (38/38);  total (38/38)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGA  >  ACB122/1806615‑1806877
                                                                                                                                   |                                                                                                                                   
gATGGGGCTGGAAGACATCAACAAGGCCTTCGACGTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGGCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCcac                                                                                                                                  <  3:155837/135‑1 (MQ=255)
        tGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTa                                                                                                                          >  2:155310/1‑135 (MQ=255)
           aaGACATCGACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCg                                                                                                                       <  8:243490/135‑1 (MQ=255)
           aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTAc                                                                                                                         <  6:166662/133‑1 (MQ=255)
           aaGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTAc                                                                                                                         <  3:342136/133‑1 (MQ=255)
            aGACATCACCAAGGCCTTCGACCTGATGCAGGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCgg                                                                                                                      <  6:223274/135‑1 (MQ=255)
                  caacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGcatcat                                                                                                                >  1:348792/1‑135 (MQ=255)
                   aacaaGGCCTTCGACCTGATGCATGACGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                               <  6:112899/135‑1 (MQ=255)
                   aacaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                               >  8:311822/1‑135 (MQ=255)
                    acaaGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                               <  7:311822/134‑1 (MQ=255)
                          ccTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGt                                                                                                        <  3:249097/135‑1 (MQ=255)
                           cTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTg                                                                                                       >  7:106144/1‑135 (MQ=255)
                             tCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                               >  2:291995/1‑125 (MQ=255)
                             tCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                               <  1:291995/125‑1 (MQ=255)
                               gaacTGTTGCATGAAGGCAAGATCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAATAGCTTCGGCGGATGCCACAATCGTTACCGGCATCATTCCAAGGTGCt                                                                                                     <  2:319732/130‑1 (MQ=255)
                                aCCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTggg                                                                                                  <  2:348792/135‑1 (MQ=255)
                                aCCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTggg                                                                                                  >  5:13084/1‑135 (MQ=255)
                                 ccTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGt                                                                                                 >  7:20013/1‑135 (MQ=255)
                                      tGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGc                                                                                              <  6:13084/133‑1 (MQ=255)
                                      tGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGa                                                                                            <  5:135981/135‑1 (MQ=255)
                                      tGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCAAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGc                                                                                              <  7:359717/133‑1 (MQ=255)
                                      tGCATGAAGGACAGAGCATCCGTAGCGGGATCCACTTCTGAGGGCTGTCATGAGCCTGGATAACATCTCCTTCCAGAAGAGCTTCGGCGGCTGCCACACGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGa                                                                                            <  8:65052/135‑1 (MQ=255)
                                        cATGAAGGCTAGAGCTTCCGTAGCGTGATCCACTTGTGAGGTCTGTCATGAGCCTGTATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACTAGCGGTACCGGCATCATTCCAAGGTGCTGGGTTGCgtga                                                                                          >  3:231497/1‑132 (MQ=255)
                                        cATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCAAAGGTGCTGGGTTGCGata                                                                                          >  8:213152/1‑135 (MQ=255)
                                              ggCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGgtgt                                                                                    >  6:151019/1‑135 (MQ=255)
                                                  agagCATCCGTAGCGGGATCCACTTCTGAGCGCGGTCCTGAGCCTGGATAACATCTCCTGCCCGAAGAGCATCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGc                                                                                <  2:330533/135‑1 (MQ=255)
                                                      cATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                               >  6:270656/1‑100 (MQ=255)
                                                      cATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCAtt                                                                                                               <  5:270656/100‑1 (MQ=255)
                                                         ccGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTAc                                                                         <  2:156588/135‑1 (MQ=255)
                                                           gTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCt                                                                       >  1:308364/1‑135 (MQ=255)
                                                           gTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCt                                                                       >  6:330476/1‑135 (MQ=255)
                                                           gTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCt                                                                       >  3:258318/1‑135 (MQ=255)
                                                            tAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTc                                                                                  >  5:116977/1‑123 (MQ=255)
                                                            tAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTc                                                                                  <  6:116977/123‑1 (MQ=255)
                                                                 tGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAg                                                                                                          <  3:58234/94‑1 (MQ=255)
                                                                 tGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAg                                                                                                          >  4:58234/1‑94 (MQ=255)
                                                                 tGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgccgcc                                                                 >  2:195277/1‑135 (MQ=255)
                                                                  gATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGAGATGGTGTTCGCTGTGTCCCTGccgccg                                                                >  6:291868/1‑135 (MQ=255)
                                                                    tCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCTGCATCATTCCAAGGt                                                                                                        >  5:271340/1‑93 (MQ=255)
                                                                    tCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGt                                                                                                        <  6:271340/93‑1 (MQ=255)
                                                                      cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                     <  8:295191/94‑1 (MQ=255)
                                                                      cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                     >  7:295191/1‑94 (MQ=255)
                                                                      cACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAgg                                                            >  6:43956/1‑135 (MQ=255)
                                                                       aCTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCTCTGTGTACCTgcc                                                                    <  7:275534/126‑1 (MQ=255)
                                                                       aCTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTgcc                                                                    >  8:275534/1‑126 (MQ=255)
                                                                                   tGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCg                                                >  2:256376/1‑134 (MQ=255)
                                                                                      cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaac                                            >  6:210110/1‑134 (MQ=255)
                                                                                      cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                                            >  1:188891/1‑135 (MQ=255)
                                                                                      cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                                            >  5:20490/1‑135 (MQ=255)
                                                                                      cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                                            >  4:23136/1‑135 (MQ=255)
                                                                                      cATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGaaaa                                            >  6:97847/1‑135 (MQ=255)
                                                                                        tGAGCCTGGATATCTTCTCGTGCCAGTAGCGCTTCGGCGGCTGCCACTAGAGTTACGTTCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                          <  8:359710/135‑1 (MQ=255)
                                                                                             cTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                                     <  2:308364/135‑1 (MQ=255)
                                                                                             cTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                                     <  7:193926/135‑1 (MQ=255)
                                                                                             cTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCg                                     <  5:112976/135‑1 (MQ=255)
                                                                                              tGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCgg                                    <  5:43956/135‑1 (MQ=255)
                                                                                                  tAACTTCTCCTGCCAGAAGAGCTTCGGCGTCTGCCACAAGCGATACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGATCGCTGTGTACCAGCAGCCGCAGCCCGAGCAGCGCGAAAAGCTGCCGGTGCt                                >  7:359710/1‑135 (MQ=255)
                                                                                                  tAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCt                                <  4:275789/135‑1 (MQ=255)
                                                                                                    aCATCTCCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGt                              >  4:342867/1‑135 (MQ=255)
                                                                                                        ctcCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCgg                                    <  1:101792/125‑1 (MQ=255)
                                                                                                        ctcCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCgg                                    >  2:101792/1‑125 (MQ=255)
                                                                                                         tcCTGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTgg                         >  8:251428/1‑135 (MQ=255)
                                                                                                            tGCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCGGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGc                        <  8:144449/133‑1 (MQ=255)
                                                                                                             gCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCa                     >  1:308112/1‑135 (MQ=255)
                                                                                                             gCCAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGCTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCa                     >  2:237194/1‑135 (MQ=255)
                                                                                                             gCCAGAAGAGCTTCGCCGGCTGCCACAAGCGTAACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGACGACGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCa                     >  5:276740/1‑135 (MQ=255)
                                                                                                               cAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc                   <  7:90474/135‑1 (MQ=255)
                                                                                                               cAGAAGAGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGc                   <  3:223740/135‑1 (MQ=255)
                                                                                                                agaagaGCTTCGGCGGCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCg                  <  7:251428/135‑1 (MQ=255)
                                                                                                                    gagCTTCGGCGGCTGCCACAAGCGTTAACGGCATCATTCCAAGGTGCTGTGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCt              <  5:56956/135‑1 (MQ=255)
                                                                                                                          cggcggCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                          <  3:56957/101‑1 (MQ=255)
                                                                                                                          cggcggCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGc                                          >  4:56957/1‑101 (MQ=255)
                                                                                                                          cggcggCTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTg        >  4:55602/1‑135 (MQ=255)
                                                                                                                           ggcggcTGCCACAAGCGTTACCGGCATCATTCCAATGTGCt                                                                                                     <  7:259925/41‑1 (MQ=255)
                                                                                                                           ggcggcTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCt                                                                                                     >  8:259925/1‑41 (MQ=255)
                                                                                                                                cTGCCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACcga  <  2:308112/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
GATGGGGCTGGAAGACATCAACAAGGCCTTCGACCTGATGCATGAAGGCAAGAGCATCCGTAGCGTGATCCACTTCTGAGGTCTGTCATGAGCCTGGATAACATCTCCTGCCAGAAGAGCTTCGGCGGCTGGCACAAGCGTTACCGGCATCATTCCAAGGTGCTGGGTTGCGATATGGTGTTCGCTGTGTACCTGCCGCCGCAGGCCGAGCAGGGCGAAAAGCTGCCGGTGCTGTACTGGCTCAGCGGCCTCACCTGCACCGA  >  ACB122/1806615‑1806877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑