Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	1,834,908	T→G	27.2%	T38P (ACC→CCC)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,834,908	0	T	G	27.2%	53.8 / 2.0	39	T38P (ACC→CCC)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (17/10);  new base G (6/6);  total (23/16)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCA  >  ACB122/1834774‑1835036                                                                                                                                        |                                                                                                                                 gACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTGCCAGGGCGGCGgt                                                                                                                                  <  5:295541/135‑1 (MQ=255)  aCGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTGCCAGGGCGGCGgtg                                                                                                                                 <  3:298175/135‑1 (MQ=255)  aCGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTGCCAGGGCGGCGgtg                                                                                                                                 <  5:352822/135‑1 (MQ=255)      ggTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCAACAGCCCGGGCGGCGGTGTCGa                                                                                                                             >  6:183625/1‑135 (MQ=255)       gTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTGCCAGGGCGGCGGTGTCGAg                                                                                                                            <  3:323923/135‑1 (MQ=255)           tAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCCAGTTCGTCATTGATGAAGGCGCAGCCCCCCAC‑AAAGCCAACTGCCAGGGCGGCGCTGTGGAggg                                                                                                                          >  8:19717/1‑133 (MQ=255)            aGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTGGAGGTCGGCATTGAGGAAGGCGCAGCCCACCAC‑AAACCCATCGGGCAGGGGGGGGGGGTGGCGGGTcc                                                                                                                       >  5:174656/1‑134 (MQ=255)                    gAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGGGTTGGGGATTGATGGAGGCGCAGCCCACCTC‑AAAGCCATCTGCCCGGGCGGCGGTGTGGAGGGGGAggcgggcg                                                                                                               >  4:281747/1‑135 (MQ=255)                     aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGGTCGTCATTGATGAAGGCGCAGACCACCGC‑AAACCCAACAGCCAGGGCGGCGGGGTGGAGGGTGAGgcgggcgg                                                                                                              >  6:63331/1‑135 (MQ=255)                     aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAATTAGTCAATGATGAAGGCGCAGACCACCAA‑AAAGCAAAAAGGCAGGGCGGCGGTGTAGCTGGTAAGgcgggcgg                                                                                                              >  3:317907/1‑135 (MQ=255)                            ggCGAGCACCCGCGGGCCAGCGGCGGCCTGGCGCTGCAGCACCGGCGCGGCGAGTGCGACATTGATGAAGGCGCACACCACCTC‑AAACCCATCTGCCCGGGCGGGGGTGTCGCGGGTCAGGCGGGCGGGCTGGaa                                                                                                       <  8:155657/135‑1 (MQ=255)                            ggCGACCAACCGCGTGCCAACGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTGCGGAATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTGCCCGGGGGGCGGTGTCGTGGGTCCGGCGGGCGGGCTGGaa                                                                                                       <  7:174164/135‑1 (MQ=255)                             ggtagcAACCGCGTGCCAGCGGCGGCAGGGCGCGGCACCACCGGCGCGAGGAGTTGGCCTTTGTTCATGGGGCACACCACCTC‑AAAGCCTTGTGCCAGGGCGGCGGTGTCGAGGGGCAGGCGTGCGGGCTGGaaa                                                                                                      <  4:187886/130‑1 (MQ=255)                               taTCAACCGGGGGCCCGCGGGGGCAAGCCGCTGCAGCACCGGCGCGTCTTGTTCTTCTTTGTCGATGCGGCACACCACATC‑AAAGCCAGTTGCCTGGGCGGGGGTGTGGTGGGTCAGGCGTGGGTGCTGGAAATg                                                                                                    <  8:282085/134‑1 (MQ=255)                                    aCCGCGTGCCAACGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTGATTGCTGAAGGCGCAGCCCCCCGC‑AAAGCCATCTGCCAGGGCGGCGGGGTCGAGGGTGAGGCGGGCGGGCGGGAAATGCAGCt                                                                                               >  8:174164/1‑135 (MQ=255)                                          tGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTGGAGTTGGGCATTGATGAAGGCGCAGACCACCGC‑AAAGCCATCTGGCCCGGCGGCGGTGTGGGCGGTCCGGGGGGCGGGCTGGCAATGCAGCGCCCggg                                                                                         >  4:214130/1‑135 (MQ=255)                                             cAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGCGTTAGGGATTGATGAAGGCGCCGCCCCACTC‑AAAGCCAGCTGCCCGGGAGGCGGGGGCGCGGGTCACGCCGGCGGGCGGGACATGCACCGCCCGGGCgg                                                                                      >  2:282438/1‑135 (MQ=255)                                                        aGGCGCTGCAGCACGGGCGCGGCGAGTTCGTCATTGATGAAGGCGCAGACCACCGC‑AAAGCCATCGGCCCGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCa                                                                           <  1:246082/135‑1 (MQ=255)                                                                           cgTCGAGTTGGTAATTGATGAAGGCGCAGACCACCTC‑AAAGCCCTCTGCCAGGGCGGCGGGGTCGAGGGTCAGGCGGGCGGGCTGGAAATTCAGCACCCCGGCGGTGCCGCCCGCAGCCATGGTGAAGCTTTCCt                                                        >  4:302159/1‑135 (MQ=255)                                                                           cgTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCt                                                        >  7:93867/1‑135 (MQ=255)                                                                             gcGAGTTCGTATTTTATGAAGGCCCACACC‑CCTCAAAAGCCACCGGCCCGGGCGGCGGGGTCGCGGGCCGGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGa                                                      <  4:3467/134‑1 (MQ=255)                                                                                aGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTTCCAGGGCGGCGGGGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCCGCCGTGGTGAAGCGTTACTGATc                                                    >  1:62881/1‑134 (MQ=255)                                                                                            tggacggCGCAGACCACTTA‑AAAGACTGCTGCCTGGGGGGCGGGGTCGCGGGTCTGGGGGGCGGGCGGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctggct                                        <  7:82445/129‑1 (MQ=255)                                                                                                           accTC‑AAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgcg                        >  1:320740/1‑135 (MQ=255)                                                                                                            ccTC‑AACGCCAGGTGCCGGGGGGGGGGTGGGGGGGGGCAGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgcgc                       <  7:350887/135‑1 (MQ=255)                                                                                                               c‑AAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCa                    >  8:252824/1‑135 (MQ=255)                                                                                                               c‑AAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCa                    >  6:277104/1‑135 (MQ=255)                                                                                                               c‑AAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTGTCCTGATCGTAGTGCTGGCTGCTGCACAACAGGGCGCGCa                    >  4:244202/1‑135 (MQ=255)                                                                                                               c‑AAAGCAATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCa                    >  3:180266/1‑135 (MQ=255)                                                                                                                   tcccAGCTGCCAGGGGGGGGGTGGCGTGGGACGGGGGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                 <  2:320740/133‑1 (MQ=255)                                                                                                                   aGCCAGCTGCCCGGGGGGCGGGGTCGAGGGTCAGGCGGGCGGGCTGGAAATGAAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                 <  2:336254/135‑1 (MQ=255)                                                                                                                     ccATCTGCCCGGGGGGGGGGGGCGTGGGGCCGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTc               <  2:127602/135‑1 (MQ=255)                                                                                                                     ccATCTGCCCGGGCGGCGGTGGCGAGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTc               <  4:44979/135‑1 (MQ=255)                                                                                                                        tCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGaa            <  7:55136/135‑1 (MQ=255)                                                                                                                          tGCCCGGGCGGGGGGGGCGAGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACt          <  2:92176/135‑1 (MQ=255)                                                                                                                           gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc         >  2:66404/1‑135 (MQ=255)                                                                                                                           gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc         >  6:4174/1‑135 (MQ=255)                                                                                                                           gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc         >  1:293679/1‑135 (MQ=255)                                                                                                                           gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc         >  7:57232/1‑135 (MQ=255)                                                                                                                           gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTc         >  3:198807/1‑135 (MQ=255)                                                                                                                              aGGGCGGGGGGGGCGCGGGGCCGGGGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCtt      <  3:214130/135‑1 (MQ=255)                                                                                                                                  cggcggTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCa  >  1:127602/1‑135 (MQ=255)                                                                                                                                       gtgtCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTc               >  4:72174/1‑117 (MQ=255)                                                                                                                                       gtgtCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTc               <  3:72174/117‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 GACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTC‑AAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCA  >  ACB122/1834774‑1835036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑