Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,835,115 T→A 5.3% Q912L (CAG→CTG)  barA ← Signal transduction histidine‑protein kinaseBarA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,835,1150TA 5.3% 248.7 / 2.2 75Q912L (CAG→CTG) barASignal transduction histidine‑protein kinaseBarA
Reads supporting (aligned to +/- strand):  ref base T (37/34);  new base A (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

TGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTG  >  ACB122/1834982‑1835244
                                                                                                                                     |                                                                                                                                 
tGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCtg                                                                                                                                  >  5:163270/1‑135 (MQ=255)
       aGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCAGTgccg                                                                                                                             >  3:52651/1‑133 (MQ=255)
         tGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAg                                                                                                                         <  8:93867/135‑1 (MQ=255)
          gctggctgCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGc                                                                                                                        >  8:195093/1‑135 (MQ=255)
               ctgctgAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCt                                                                                                                   >  6:241108/1‑135 (MQ=255)
               ctgctgAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCAGTGCCGCCAGCCGGCt                                                                                                                   >  5:70034/1‑135 (MQ=255)
                 gctgAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTAGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGa                                                                                                                 >  1:39845/1‑135 (MQ=255)
                   tgAACAACAGGGCGCGCCTGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGGCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATc                                                                                                               <  1:282438/135‑1 (MQ=255)
                            gggCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTcc                                                                                                      <  7:252824/135‑1 (MQ=255)
                               cgcgcATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGt                                                                                                   >  1:214798/1‑135 (MQ=255)
                                  gcATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGGATGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCTCTCAATCTGGCATGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCa                                                                                                <  4:317907/135‑1 (MQ=255)
                                    aTGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCAt                                                                                               >  6:20712/1‑134 (MQ=255)
                                                     cattGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCt                                                                             >  7:151063/4‑135 (MQ=255)
                                                     cAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCt                                                                             >  3:136745/1‑135 (MQ=255)
                                                       aTGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTgg                                                                           <  5:344081/135‑1 (MQ=255)
                                                           aTGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCa                                                                                                     <  7:109158/105‑1 (MQ=255)
                                                           aTGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCa                                                                                                     >  8:109158/1‑105 (MQ=255)
                                                           aTGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGAt                                                                       >  5:208542/1‑135 (MQ=255)
                                                            tGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCAGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGAtt                                                                      <  4:52651/135‑1 (MQ=255)
                                                            tGTCTTGGGGGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGAtt                                                                      <  7:19717/135‑1 (MQ=255)
                                                             gTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTg                                                                     >  2:97121/1‑135 (MQ=255)
                                                             gTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTg                                                                     >  6:244833/1‑135 (MQ=255)
                                                             gTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTg                                                                     >  3:57410/1‑135 (MQ=255)
                                                             gTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTg                                                                     >  3:43635/1‑135 (MQ=255)
                                                             gTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTCATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTg                                                                     >  4:88115/1‑135 (MQ=255)
                                                                ttGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCa                                                                                                     <  1:138106/100‑1 (MQ=255)
                                                                ttGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCa                                                                                                     >  2:138106/1‑100 (MQ=255)
                                                                ttGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTcc                                                                  >  7:316337/1‑135 (MQ=255)
                                                                  gggtgggtCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTgg                                                                           >  7:241557/1‑124 (MQ=255)
                                                                  gggtgggtCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTgg                                                                           <  8:241557/124‑1 (MQ=255)
                                                                  gggtgggtCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTCCTGCGCGGGGGGATTGTCCTg                                                                >  6:211464/1‑135 (MQ=255)
                                                                   ggtgggtCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTcc                                                                  <  1:97121/132‑1 (MQ=255)
                                                                   ggtgggtCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTcc                                                                  <  5:244833/132‑1 (MQ=255)
                                                                     tgcgtCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCtt                                                             >  7:64906/4‑135 (MQ=255)
                                                                      gggtCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGCTCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCt                                                              >  6:175495/1‑133 (MQ=255)
                                                                      gggtCACTGCCGGCCATGGCATCCCAAGGGGGGCCCATAT‑CCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCa                                                           <  5:183625/135‑1 (MQ=255)
                                                                       ggtCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCa                                                           <  5:277104/135‑1 (MQ=255)
                                                                           aCTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCgcgc                                                                                                           >  2:313462/1‑83 (MQ=255)
                                                                           aCTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCgcgc                                                                                                           <  1:313462/83‑1 (MQ=255)
                                                                           aCTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCaa                                                       >  6:151252/1‑135 (MQ=255)
                                                                           aCTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCAGACAGCCGGCTGATCGCGCTGTCAAGATCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTACAGCaa                                                       >  1:294518/1‑135 (MQ=255)
                                                                               ccTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGtc                                                   <  7:12195/135‑1 (MQ=255)
                                                                                      aGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGc                                            <  4:105991/135‑1 (MQ=255)
                                                                                      aGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGc                                            <  1:289824/135‑1 (MQ=255)
                                                                                          aTGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTg                                        <  2:7394/135‑1 (MQ=255)
                                                                                            gCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGac                                      <  7:195093/135‑1 (MQ=255)
                                                                                              ccAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGCTCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCGGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGacac                                    >  1:137675/1‑135 (MQ=255)
                                                                                              ccAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGacac                                    <  2:293679/135‑1 (MQ=255)
                                                                                                aGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGc                                  >  7:46689/1‑135 (MQ=255)
                                                                                                 gCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCt                                 <  5:20712/135‑1 (MQ=255)
                                                                                                    tGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGcc                              <  6:163270/135‑1 (MQ=255)
                                                                                                     ggCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATc                                                                                                               <  3:284415/53‑1 (MQ=255)
                                                                                                     ggCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATc                                                                                                               >  4:284415/1‑53 (MQ=255)
                                                                                                     ggCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGtc                                                   <  5:274887/113‑1 (MQ=255)
                                                                                                     ggCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGtc                                                   >  6:274887/1‑113 (MQ=255)
                                                                                                     ggCCCAGATAAACTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGTCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGAGTCAGCAAGGTCTCGCTGCCTGGCCACTCTGccc                             >  1:368421/1‑135 (MQ=255)
                                                                                                          aGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCctgctg                                                                                  >  8:86180/1‑77 (MQ=255)
                                                                                                          aGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCctgctg                                                                                  <  7:86180/77‑1 (MQ=255)
                                                                                                            aTCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTg                      <  8:316337/135‑1 (MQ=255)
                                                                                                              ccccTCAAGCGCTCAATCTGGCCAGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCg                    <  6:70034/135‑1 (MQ=255)
                                                                                                               cccTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATc                                                                                                               <  3:204367/43‑1 (MQ=255)
                                                                                                               cccTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATc                                                                                                               >  4:204367/1‑43 (MQ=255)
                                                                                                                ccTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGc                  <  4:43635/135‑1 (MQ=255)
                                                                                                                ccTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGc                  <  3:244202/135‑1 (MQ=255)
                                                                                                                    aaGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGac                                      <  1:48071/111‑1 (MQ=255)
                                                                                                                    aaGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGac                                      >  2:48071/1‑111 (MQ=255)
                                                                                                                    aaGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCAcgc              >  6:69351/1‑135 (MQ=255)
                                                                                                                    aaGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCAcgc              >  6:371822/1‑135 (MQ=255)
                                                                                                                      gcgcTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCAcgccg            <  5:241108/135‑1 (MQ=255)
                                                                                                                      gcgcTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCAcgccg            <  6:208542/135‑1 (MQ=255)
                                                                                                                        gcTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCa          <  2:214798/135‑1 (MQ=255)
                                                                                                                            aaTCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAg      >  7:47880/1‑135 (MQ=255)
                                                                                                                            aaTCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAg      >  1:103233/1‑135 (MQ=255)
                                                                                                                            aaTCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGAGTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAg      >  5:129446/1‑135 (MQ=255)
                                                                                                                             aTCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGc     >  1:88333/1‑135 (MQ=255)
                                                                                                                                   ccTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTg  <  5:4174/132‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
TGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAATGCATGTCTTGGGTGGGTCACTGCCTGTCAAGGCATGCCCAGCGTGGCCCAGATCCCCTCAAGCGCTCAATCTGGCCTGTGCCGCCAGCCGGCTGATCGCGCTGTCCAGTTCATCCAGGGCCTGCTGCGCCTGGGGATTGTCCTGCTTCAGCAAGGTCTCGCTGCGTTGACACGCTGCCCGTAACTGCGGCACGCCGCAATAGCGTG  >  ACB122/1834982‑1835244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑