Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 1,881,160 G→A 5.7% F723F (TTC→TTT)  rcsC_2 ← Sensor histidine kinase RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,881,1600GA 5.7% 216.1 / 2.1 70F723F (TTC→TTT) rcsC_2Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base G (34/32);  new base A (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

TTGCTCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCTCGGCGTTGTAGCGGATGATGTCGGCAATCGGTC  >  ACB122/1881028‑1881290
                                                                                                                                    |                                                                                                                                  
ttGCTCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGaa                                                                                                                                  <  5:334048/135‑1 (MQ=255)
   cTCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACa                                                                                                                                <  5:303095/134‑1 (MQ=255)
   cTCAAGGCGTTAGTTTGCATCGCGCAGTGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCTCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACa                                                                                                                                <  6:306366/134‑1 (MQ=255)
    tCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGc                                                                                                                              >  1:164222/1‑135 (MQ=255)
    tCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGc                                                                                                                              <  1:21709/135‑1 (MQ=255)
    tCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGAAACAGc                                                                                                                              >  3:367962/1‑135 (MQ=255)
    tCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGAAACAGc                                                                                                                              >  3:373957/1‑135 (MQ=255)
    tCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGGTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGc                                                                                                                              >  1:45516/1‑135 (MQ=255)
     cAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGcc                                                                                                                             <  7:224594/135‑1 (MQ=255)
      aaGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCg                                                                                                                            >  2:212029/1‑135 (MQ=255)
           gttcgttGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACACAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTc                                                                                                                         >  6:274199/1‑133 (MQ=255)
                ttGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGACACGCCGCGCGgaggag                                                                                                                  >  6:22997/1‑135 (MQ=255)
                ttGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGgaggag                                                                                                                  >  5:147722/1‑135 (MQ=255)
                ttGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCCCCCGGCATCGCGTTGCCGATCAGCGCGATCACCCGCCCATTGGGGGACAGCCCCTCGgaggag                                                                                                                  >  8:226703/1‑135 (MQ=255)
                ttGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCAGGACAAAGCCACCGCCCGGCATCGGGTTGCAGATCAGCTCGATCACCCGGCCATTGGGGACCAGCCGCTCGgaggag                                                                                                                  >  5:141201/1‑135 (MQ=255)
                 tGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGGGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGt                                                                                                                 <  8:319075/135‑1 (MQ=255)
                   gcttggcgcTGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGGGGGTTAAGCTAATGACAAAGCCACCGCCGGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGTGGAACTGGCGCTCGGAGGAGTgc                                                                                                               <  6:24703/130‑1 (MQ=255)
                   gCATCGCGCCGGGCCGGCTCGGCCGCGCGCACCGGGGGGATGTCGGGGACGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGGgc                                                                                                               <  5:48097/135‑1 (MQ=255)
                   gCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTgc                                                                                                               <  8:19996/135‑1 (MQ=255)
                   gCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGAGGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCTTCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTgc                                                                                                               <  3:320394/135‑1 (MQ=255)
                   gCAGCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGCCAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTgc                                                                                                               <  8:334598/135‑1 (MQ=255)
                    cagcgcgCAGGGCCTGCGCGGCCGCGCGGAACGGGGTGATGGCGGTGAAGCTCATGACAACGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGGgc                                                                                                               <  6:141201/131‑1 (MQ=255)
                    cATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGAAACAGCCGCTCGGAGGAGTgc                                                                                                               <  4:367962/134‑1 (MQ=255)
                    cATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGAAACAGCCGCGCGGAGGAGTgc                                                                                                               <  4:373957/134‑1 (MQ=255)
                    cATCGCGCAGGGCCTGCTCGGCCGCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTgc                                                                                                               <  6:147722/134‑1 (MQ=255)
                     aTCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTgcgc                                                                                                             >  6:320395/1‑135 (MQ=255)
                            aGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACCGCCGCTCGGAGGAGTGCGCGCGGccc                                                                                                      >  5:31838/1‑135 (MQ=255)
                             gggCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCt                                                                                                     <  5:102867/135‑1 (MQ=255)
                                ccTGCTCGGCCTCGCGCAACGGGGTGAGGTCGGTGAAGCTCATGACAAACCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACACCCGCTCGGAGGAGTGCGCGCGGCCCTGgc                                                                                                  <  1:205603/135‑1 (MQ=255)
                                 cTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGCTCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGgcg                                                                                                 >  3:2256/1‑135 (MQ=255)
                                 cTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGCTCAGCTCGATCACCCGGCCAGTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGgcg                                                                                                 >  7:164313/1‑135 (MQ=255)
                                      cGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCAt                                                                                              >  2:65313/1‑133 (MQ=255)
                                      cGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATcc                                                                                            >  4:293109/1‑135 (MQ=255)
                                           tCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCt                                                                                                     >  7:231385/1‑121 (MQ=255)
                                           tCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCt                                                                                                     <  8:231385/121‑1 (MQ=255)
                                           tCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGt                                                                                         >  5:193261/1‑133 (MQ=255)
                                           tCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGc                                                                                       <  7:216203/135‑1 (MQ=255)
                                           tCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCACCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGt                                                                                         >  1:5074/1‑133 (MQ=255)
                                                 aaCGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGCTCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCCTCCAGTGCAgccgc                                                                                 >  1:160794/1‑135 (MQ=255)
                                                 aaCGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAgccgc                                                                                 >  7:66023/1‑135 (MQ=255)
                                                  aCGGGGTGATGTCGGTCAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTGGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAgccgcc                                                                                <  7:226703/135‑1 (MQ=255)
                                                  aCGGGGTGATGGCGGTGACGCGCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAgccgcc                                                                                <  4:278567/135‑1 (MQ=255)
                                                  aCGGGGTGAGGTCCGGGAAGCTCATGACATAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAgccgcc                                                                                <  3:219150/135‑1 (MQ=255)
                                                    ggggggAGGTCGGGGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCcgccgc                                                                              <  2:32628/135‑1 (MQ=255)
                                                      ggTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCgcgc                                                                            >  6:136873/1‑135 (MQ=255)
                                                      ggTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCgcgc                                                                            >  6:136868/1‑135 (MQ=255)
                                                       gTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGcc                                                                           <  6:193261/135‑1 (MQ=255)
                                                            gTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATg                                                                      <  5:136192/135‑1 (MQ=255)
                                                            gTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATg                                                                      <  5:136190/135‑1 (MQ=255)
                                                              cGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTgc                                                                                                               <  6:307326/92‑1 (MQ=255)
                                                              cGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTgc                                                                                                               >  5:307326/1‑92 (MQ=255)
                                                                  gacgCTCATGACAACGCCACCGCCCGGCAGCGGGTGGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCtg                                                                <  1:93685/132‑1 (MQ=255)
                                                                   aaGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCtgt                                                               >  3:99878/1‑135 (MQ=255)
                                                                   aaGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCtgt                                                               >  5:290004/1‑135 (MQ=255)
                                                                   aaGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCtgt                                                               >  7:117983/1‑135 (MQ=255)
                                                                       tCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGaa                                                                                                                                  <  1:97734/64‑1 (MQ=255)
                                                                       tCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGaa                                                                                                                                  >  2:97734/1‑64 (MQ=255)
                                                                         aTGACAACGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCg                                                         <  3:346232/135‑1 (MQ=255)
                                                                         aTGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCg                                                         >  8:204518/1‑135 (MQ=255)
                                                                                 gCCACCGCCCGGCATCGGGTGGCCGATCAGCTCGAGCACCCGGCCATTGGGGAACAGCCGCGCGGAGGAGGGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGcc                                                 <  5:183283/135‑1 (MQ=255)
                                                                                   cACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCTCCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTCCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGc                                               >  4:370929/1‑135 (MQ=255)
                                                                                        cccGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAgg                                          >  7:37629/1‑135 (MQ=255)
                                                                                        cccGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGACTCGCCGGGGCCGCACAgg                                          >  2:148407/1‑135 (MQ=255)
                                                                                            gCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGACTCGCCGGGGCCGCACAGGCcgc                                      <  1:148407/135‑1 (MQ=255)
                                                                                               tCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCt                                   >  7:114405/1‑135 (MQ=255)
                                                                                               tCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCt                                   >  1:231246/1‑135 (MQ=255)
                                                                                                  ggTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCTCg                                 <  6:95896/134‑1 (MQ=255)
                                                                                                          aTCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCTCGGCGTTGTAg                        >  4:36934/1‑135 (MQ=255)
                                                                                                                          ggCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCTCGGCGTTGTAGCGGATGATGTCGGCaa        >  6:212452/1‑135 (MQ=255)
                                                                                                                           gCCATTGGGGAACAGCCGCTCGGAGGAGTTCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCTCGGCGTTGTAGCGGATGATGTCGGCAAt       <  7:377368/135‑1 (MQ=255)
                                                                                                                                 ggggAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCTCGGCGTTGTAGCGGATGATGTCGGCAATCGGTc  <  3:293109/134‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
TTGCTCAAGGCGTTCGTTGGCATCGCGCAGGGCCTGCTCGGCCTCGCGGAACGGGGTGATGTCGGTGAAGCTCATGACAAAGCCACCGCCCGGCATCGGGTTGCCGATCAGCTCGATCACCCGGCCATTGGGGAACAGCCGCTCGGAGGAGTGCGCGCGGCCCTGGCGCATCCAGTGCAGCCGCCGCGCCACATGCACCTGTGCCTCGCCGGGGCCGCACAGGCCGCGCTCGGCGTTGTAGCGGATGATGTCGGCAATCGGTC  >  ACB122/1881028‑1881290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑