Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 2,313,489 G→A 5.8% S270L (TCG→TTG)  gltC_2 ← HTH‑type transcriptional regulator GltC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,313,4890GA 5.8% 212.4 / 3.3 69S270L (TCG→TTG) gltC_2HTH‑type transcriptional regulator GltC
Reads supporting (aligned to +/- strand):  ref base G (29/36);  new base A (2/2);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

TGCTCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTG  >  ACB122/2313363‑2313617
                                                                                                                              |                                                                                                                                
tGCTCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGc                                                                                                                           >  8:288933/1‑134 (MQ=255)
   tCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAg                                                                                                                       <  8:112433/135‑1 (MQ=255)
   tCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAg                                                                                                                       >  7:85105/1‑135 (MQ=255)
   tCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAg                                                                                                                       <  3:240020/135‑1 (MQ=255)
     cGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCCCCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAgg                                                                                                                      >  8:212606/1‑134 (MQ=255)
     cGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGGGAGTGGGCCAAGGc                                                                                                                     >  3:43503/1‑135 (MQ=255)
         aGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGCCAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGagg                                                                                                                 >  3:61797/1‑133 (MQ=255)
             ccTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCCCCAGGTCGCGCATGCAGGCGGCGGCGCACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGc                                                                                                             >  6:309873/1‑135 (MQ=255)
                    ctctATCAAGGTTCCTTTCCAGAGCTCGCTGCGCACCGAATCCACCAGCAGTGCGCGCACCAGGGCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTTGTCCAAGGCGATTGTGCGGGTGAt                                                                                                      <  3:297798/135‑1 (MQ=255)
                    cTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCCCCAGGTCGCGCATGCAGGCGGCGGCGACCGACCGCGGCGTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGAt                                                                                                      >  8:318342/1‑135 (MQ=255)
                    cTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGAt                                                                                                      <  8:234033/135‑1 (MQ=255)
                    cTCGATCAAGGTTCCTTTCCAGCGCTCGCGGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCAGGCAGGCGGCGGCGAACGACAGCGGCGTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGAt                                                                                                      <  5:158893/135‑1 (MQ=255)
                    cTCGATCAAGGTGCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGAt                                                                                                      <  4:82169/135‑1 (MQ=255)
                    cTCGATCAAGGGTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGGGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGAt                                                                                                      <  5:239074/135‑1 (MQ=255)
                         tCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAgg                                                                                                                      <  8:226085/114‑1 (MQ=255)
                         tCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGcgcg                                                                                                 <  4:352323/135‑1 (MQ=255)
                         tCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCGTGTGCTGCGAGTGGGCCAAgg                                                                                                                      >  7:226085/1‑114 (MQ=255)
                         tCAAGGTTCCTTAGCAGCTCGGGCTGCGGACCGCATCCACCAGCCGTGGTCGCCCTATGTCGCGCATGCAGGCGGCGGCGAACGACATCGTCTTGTGCTGCGAGTGGGGCAAGGCGATGGTGCGTGTGATGcgcg                                                                                                 <  8:325704/135‑1 (MQ=255)
                         gcAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCGGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGcgcg                                                                                                 <  3:56196/134‑1 (MQ=255)
                                    ttCCAGCGCTCGCTTCGCACCGCATCCACCAGCAGTGCGCGCCCCAGGTCGCGCATGCAGGCGGCGGCGCACGCCACCGGCGTGTGCCGCGAGGGGGGCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTa                                                                                        >  1:348340/1‑133 (MQ=255)
                                          cgctcgctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCcgc                                                                                <  7:228394/135‑1 (MQ=255)
                                          cgctcgctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCcgc                                                                                >  8:351899/1‑135 (MQ=255)
                                          cgctcgctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCGTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCcgc                                                                                >  5:107864/1‑135 (MQ=255)
                                                         gCATCCACCCGCAGTGCGCGCACCAGGTCGCGCAGGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGcgcg                                                                 <  2:10834/135‑1 (MQ=255)
                                                         gCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGcgcg                                                                 <  7:239571/135‑1 (MQ=255)
                                                             ccaccaGCAGTGCGCGCCCCAGGTCGCGCCTGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGCTGGTGCGGGTGATGCGCGGTTCCACTATCTGCCGCGCGCTGGCAGGCGCGGCCa                                                             >  4:46354/1‑135 (MQ=255)
                                                             ccaccaGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCGTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCa                                                             >  4:16426/1‑135 (MQ=255)
                                                             ccaccaGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACACCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTACCACTATCCGCCGCACGCTGGCAGGCGCGGCCa                                                             >  1:322012/1‑135 (MQ=255)
                                                                       tGCGCGCACCAGGTCGCGCATGCAGGAGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTTGGCCAAGGCGATGGTGGGGGTGATGCTCGGTTCCACTATCAGGCGCGCGCTGGCAGGCTCGGCCAGATCcagcag                                                   <  6:82892/135‑1 (MQ=255)
                                                                          gcgcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGAt                                                                                                      >  7:59895/1‑81 (MQ=255)
                                                                          gcgcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGAt                                                                                                      <  8:59895/81‑1 (MQ=255)
                                                                          gcgcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAgcgg                                                >  8:327553/1‑135 (MQ=255)
                                                                            gcACCAGGTCGCGCATGCAGGCGGCGGCGCACGCCAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGCTGCGCGGTTCCACTATCAGCCCCCCGCTGGCAGGCGCGGCCACCTCCAGCCgcggcg                                              >  7:104708/1‑135 (MQ=255)
                                                                            gcACCAGGTCGCGCATGCAGGCGGCGGCGCACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAgcggcg                                              >  2:94563/1‑135 (MQ=255)
                                                                            gcACCAGGTCGCGCATGCAGGCGGCGGCGAACGCCAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGGTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGCTCCAGCAgcggcg                                              >  7:182722/1‑135 (MQ=255)
                                                                            gcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCAAGTggg                                                                                                                            >  5:44513/1‑57 (MQ=255)
                                                                            gcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCAAGTggg                                                                                                                            >  5:49605/1‑57 (MQ=255)
                                                                            gcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCAAGTggg                                                                                                                            <  6:49605/57‑1 (MQ=255)
                                                                            gcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCAAGTggg                                                                                                                            <  6:44513/57‑1 (MQ=255)
                                                                            gcACCAGGTCGCCCATGCCGCCGGAGGCGAACGAAAGCCGCGTGTGCTGCGCGTTGGGCAAGGCGATGGTTCGGGGGAGTCGAGGTTCCCCTATCACCCCCCCGCTAGCAGGCGCGGCCAGATCCACCCgccgcg                                              >  2:135631/1‑135 (MQ=255)
                                                                             cACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGcggcgg                                             <  5:194602/135‑1 (MQ=255)
                                                                             cACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGcggcgg                                             <  8:88652/135‑1 (MQ=255)
                                                                             cACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGcggcgg                                             <  2:79505/135‑1 (MQ=255)
                                                                              aCCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGcggcgg                                             >  8:298367/1‑134 (MQ=255)
                                                                                    tCGCGCATGCAGGCGGCGGCGAACGACAGAGTCTTTTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGCTCCAGCAGCGGCGGCCAGTTg                                      >  1:177506/1‑135 (MQ=255)
                                                                                     cgcgCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGc                                     >  1:60536/1‑135 (MQ=255)
                                                                                          aTGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGAtg                                <  8:368732/135‑1 (MQ=255)
                                                                                               ggcggcggcGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGgc                           <  5:285328/135‑1 (MQ=255)
                                                                                               ggcggcggcGAACGACAGCGGCTTGGGCTGCGAGGGGGCCACGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCCGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGgc                           <  5:273976/135‑1 (MQ=255)
                                                                                                        gAACGACAGCGGCTTGTGCGGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGGTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcc                  <  8:104708/135‑1 (MQ=255)
                                                                                                        caccGACAGCGGCTGGGGCGGCGAGTGGGCCACGGCGAGGGGGCGGGGGAGGCGCGGTTCCACTATCCGCCGCGCGCGGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcc                  <  5:14479/132‑1 (MQ=255)
                                                                                                         accgccAGCGGCTTGTGCTGCGAGTGGGCCACGGCGAGGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcca                 <  3:279421/130‑1 (MQ=255)
                                                                                                         accgccAGCGGCTTGTGCGGCGTGTGGGCCAAGGGGATGGGGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCCGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcca                 <  5:33732/130‑1 (MQ=255)
                                                                                                         aaCGACAGGGGCTTGTGCTGCGAGTGGGCCACGGCGAGGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGACAGTTGCTGATGGTGGCCgccaggcca                 <  1:74436/135‑1 (MQ=255)
                                                                                                         aaCGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCTGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcca                 <  3:46354/135‑1 (MQ=255)
                                                                                                         aaCGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcca                 <  4:120850/135‑1 (MQ=255)
                                                                                                         aaCGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcca                 <  1:30873/135‑1 (MQ=255)
                                                                                                         aaCGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcca                 <  3:16426/135‑1 (MQ=255)
                                                                                                              cAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGAGGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACg            <  1:55727/135‑1 (MQ=255)
                                                                                                                   gCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCTGGTGATGCGCGGTTCCACAAGCAGCCGCGCGCTGGCAGTCGCGGCGAGATCCAGCATCGGCGTCCAGTGGCTGATGGTGGCCGACAGGCAAGCACGGACCa       <  4:310675/135‑1 (MQ=255)
                                                                                                                    cTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAgcggcg                                              >  1:240893/1‑95 (MQ=255)
                                                                                                                    cTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAgcggcg                                              <  2:240893/95‑1 (MQ=255)
                                                                                                                    cTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAg      >  8:127974/1‑135 (MQ=255)
                                                                                                                    cTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAg      >  5:179362/1‑135 (MQ=255)
                                                                                                                       gtgCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCCGCACGGACCAGGCt   >  6:250398/1‑135 (MQ=255)
                                                                                                                       gtgCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCt   >  4:156896/1‑135 (MQ=255)
                                                                                                                         gctgcGAGGGGGCCAAGGCGATGGTGCGGGGGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTg  <  3:269242/134‑1 (MQ=255)
                                                                                                                           tgcGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcca                 >  4:275701/1‑117 (MQ=255)
                                                                                                                           tgcGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggcca                 <  3:275701/117‑1 (MQ=255)
                                                                                                                              |                                                                                                                                
TGCTCCGCCAGGGCCTCGCGCTCGATCAAGGTTCCTTTCCAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAGGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTG  >  ACB122/2313363‑2313617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑